[Leish-l] CL Antibodies (UNCLASSIFIED)

[LRU-Jericho]

I have worked on antibodies in serum from CL patients in Jericho. I used L. 
major for coating. Only 32% of CL confirmed cases were positive by ELISA. All 
depends on duration of lesion and corresponding immune response.
So far Serology for CL is of limited use.

Amer
Jericho




________________________________
From: "Rowton, Edgar D Dr CIV USA MEDCOM WRAIR" <EDGAR.ROWTON at US.ARMY.MIL>
To: jeffrey shaw <jayusp at hotmail.com>; Leish-L <leish-l at lineu.icb.usp.br>
Sent: Tue, May 17, 2011 7:57:17 PM
Subject: Re: [Leish-l] CL Antibodies (UNCLASSIFIED)

Classification:  UNCLASSIFIED
Caveats: NONE

The biggest issue we've had, even with homologous antigen, is that antibody 
detection on CL cases is inconsistent.  Both between individuals and at 
different time points with the same individual.  These observations were made 
many years ago.  With new and more sensitive techniques things can change.

Ed Rowton
WRAIR

-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] 
On Behalf Of jeffrey shaw
Sent: Monday, May 16, 2011 3:55 PM
To: Leish-L
Subject: [Leish-l] CL Antibodies

The accepted idea of no antibodies in cutaneous leishamaniasis is incorrect. 
They definitely do occur in some but not all cases. However, they may be missed 
if the wrong antigen is used. The greatest chance of detecting them is to use 
the homologous antigen.
Jeffrey Shaw
Classification:  UNCLASSIFIED
Caveats: NONE

_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lineu.icb.usp.br/pipermail/leish-l/attachments/20110519/c246996c/attachment.htm>