<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:arial,helvetica,sans-serif;font-size:12pt">I have worked on antibodies in serum from CL patients in Jericho. I used L. major for coating. Only 32% of CL confirmed cases were positive by ELISA. All depends on duration of lesion and corresponding immune response.<br>So far Serology for CL is of limited use.<br><br>Amer<br>Jericho<div><br></div><div style="font-family:arial, helvetica, sans-serif;font-size:12pt"><br><div style="font-family:arial, helvetica, sans-serif;font-size:10pt"><font size="2" face="Tahoma"><hr size="1"><b><span style="font-weight: bold;">From:</span></b> "Rowton, Edgar D Dr CIV USA MEDCOM WRAIR" <EDGAR.ROWTON@US.ARMY.MIL><br><b><span style="font-weight: bold;">To:</span></b> jeffrey shaw <jayusp@hotmail.com>; Leish-L <leish-l@lineu.icb.usp.br><br><b><span style="font-weight: bold;">Sent:</span></b> Tue, May 17,
2011 7:57:17 PM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: [Leish-l] CL Antibodies (UNCLASSIFIED)<br></font><br>
Classification: UNCLASSIFIED<br>Caveats: NONE<br><br>The biggest issue we've had, even with homologous antigen, is that antibody detection on CL cases is inconsistent. Both between individuals and at different time points with the same individual. These observations were made many years ago. With new and more sensitive techniques things can change.<br><br>Ed Rowton<br>WRAIR<br><br>-----Original Message-----<br>From: <a ymailto="mailto:leish-l-bounces@lineu.icb.usp.br" href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> [mailto:<a ymailto="mailto:leish-l-bounces@lineu.icb.usp.br" href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>] On Behalf Of jeffrey shaw<br>Sent: Monday, May 16, 2011 3:55 PM<br>To: Leish-L<br>Subject: [Leish-l] CL Antibodies<br><br>The accepted idea of no antibodies in cutaneous leishamaniasis is incorrect. They definitely do occur in some but not
all cases. However, they may be missed if the wrong antigen is used. The greatest chance of detecting them is to use the homologous antigen.<br>Jeffrey Shaw<br>Classification: UNCLASSIFIED<br>Caveats: NONE<br><br>_______________________________________________<br>Leish-l mailing list<br><a ymailto="mailto:Leish-l@lineu.icb.usp.br" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br><span><a target="_blank" href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a></span><br></div></div>
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