[Leish-l] CL Antibodies

[Jake Jacobson]

In our paper from 1983 we found that for indirect 
immunofluorescence.studies, a heterologous  L. donovani antigen gave 
higher titres than a homologous L. major antigen for sera from 
vaccinated individuals.

GREEN, M.S., KARK, J.D., GREENBLATT, C.L., LONDNER, M.V., 
FRANKENBURG, S.  and JACOBSON, R.,L. (1983).  The cellular and 
humoral immune response in subjects vaccinated against cutaneous 
leishmaniasis using Leishmania tropica major 
promastigotes.  Parasit.  Immunol. 5 337-344.

Summary In vitro lymphocyte transformation was studied in 24 subjects 
12 months after vaccination with live Leishmania tropica major 
vaccine, in 10 normal control subjects and in three controls residing 
in an endemic area. The vaccines had lesions in various stages of 
clinical development. Lymphocytes from all the subjects were studied 
for their response to stimulation with L. tropica major 
promastigotes. Lymphocytes of vaccinated subjects responded to low 
concentrations of antigen whereas the lymphocyte response of the 
controls tended to be depressed by the same concentration of the 
antigen. Serum from each subject was subjected to a study of the 
humoral antibody titre against L. tropica major and L. donovani using 
indirect immunofluorescence. A humoral response to L. donovani was 
present in a majority of vaccinees who had developed a positive 
lesion whereas no such response was present in any of the controls. 
The data suggest that high humoral responses were accompanied by 
relatively low cell-mediated responses and vice versa. No significant 
humoral response to L. tropica major could be demonstrated in any of 
the subjects. A combination of both the cell-mediated and humoral 
mechanisms may participate in the immune response although their 
usefulness in the assessment of the protectivity of leishmania 
vaccines has not been established.

Jake Jacobson



The accepted idea of no antibodies in cutaneous leishamaniasis is 
incorrect. They definitely do occur in some but not all cases. 
However, they may be missed if the wrong antigen is used. The 
greatest chance of detecting them is to use the homologous antigen.
Jeffrey Shaw

Imagination was given to man to compensate him for what he is not; a 
sense of humor to console him for what he is.


Dr.R.L.Jacobson MPH PhD
Department of Molecular Genetics and Microbiology,
The Institute of Medical Research Canada - Israel.
The Kuvin Center For The Study Of Tropical and Infectious Diseases
The Hebrew University-Hadassah Medical School
POB 12272, Jerusalem, 91120
Israel
Telephone 972-2-6758077
Fax          972-2-6757425
Mobile 054-4970731
NEW:
VOIP (from USA)  415-963-9801   (up to 15:00hrs EST) 
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