[Leish-l] Leish-l Digest, Vol 68, Issue 4
Abdallah Samy
asamy.ku at gmail.com
Thu Sep 5 13:41:29 BRT 2013
Dear Farrokh
I do not expect that the disease would not eventually relapse if not treated
further. i guess that we have experienced similar observations for *
Leishmania* infection in the spleen of naturally infected gerbil collected
from Sinai. The parasite was identified as *L. tropica. *The rodent died
within 24 hrs of collection, and with the dissection, we reported
spleenomegaly, and the spleen was really more dark in color. In our
culturing trials we just moved the spleen to petridish and we observed that
the cells released like granules. The samples has also high parasite loads.
I see from this example that the rat can not even survive for along time,
and using the experimental model, we get the same results after 170 days,
and the mice can not recover from the infection. I attached here a copy of
the symptoms. I am not immunologist but this is the observation that i got
in my study. I plan to publish this information. So, any helpful comments,
it will be appreciated.
Best regards
Abdallah
Abdallah M. Samy
Division of Vector Biology and Control
Entomology Department
Faculty of Science
Ain Shams University
Abbassia 11566, Cairo, Egypt
Cell phone: +1-202-213-5135
On Thu, Sep 5, 2013 at 7:50 AM, <leish-l-request at lineu.icb.usp.br> wrote:
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> Today's Topics:
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> 1. Re: spleen (Fernando Jos? Andrade Narv?ez)
> 2. Re: spleen (Carlos Costa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 30 Aug 2013 12:33:16 -0500
> From: "Fernando Jos? Andrade Narv?ez" <anarvaez at uady.mx>
> Subject: Re: [Leish-l] spleen
> To: "Leish-L" <leish-l at lineu.icb.usp.br>
> Message-ID:
> <25f8ee768329e88401beeb41b86b8c4c.squirrel at webmail.uady.mx>
> Content-Type: text/plain;charset=iso-8859-1
>
> Dear lesihmaniacs,
> I agree with all previous comments and suggestions. It is a very good
> question but it is important to remark that the spleen is an organ of the
> immune system and play different roles. In a recent paper, Chin Med J
> (Engl). 2013 Jun;126(12):2354-60. Splenectomy protects experimental rats
> from cerebral damage after stroke due to anti-inflammatory effects.
> Zhang BJ, Men XJ, Lu ZQ, Li HY, Qiu W, Hu XQ, the authors discussed
> antinflammatory effects of spelenctomy. Moreover the spleen is very
> important in "humoral" antibodies-response which is increased in VL.
> Another issue is to point out regarding the possibility of problems after
> splenctomy described in the literature.
> Best regards,
> Fernando J. Andrade-Narvaez, MD, PhD
> Chairman Bioethics Commission, State of Yucatan, Mexico,
> Research Bioethics Committee Chairman
> Head of Immunology Laboratory
> Centro de Investigaciones Regionales "Dr. Hideyo Noguchi"
> Universidad Aut?noma de Yucat?n,
> Ave. Itz?es No. 490x59 CP 97000
> M?rida, Yucat?n, M?xico
> Tel.: 52 999 924-6412 ext. 1156
> Fax: 52 999 923-6120
> E-mails: anarvaez at uady.mx, fandrade47 at gmail.com
>
> > Dear Carlos,> Your idea of IL-10 is certainly a good guess but the
> spleen is loaded with
> > undifferentiated monocytic cells that can be infected by Leishmania but
> > can not be activated to kill the parasites. We hypothesized and called
> > these cells "Safe Targets" that are responsible for perpetuating
> infection
> > even after recovery from disease - based on mouse models.
> > Do you think splenectomy cures or just reduces the parasite load, anemia
> > and also IL-1 so patients feel better but would eventually relapse if not
> > treated further?
> > Best wishes
> > Farrokh
> >
> > Sent from my DNDi iPhone
> > +41(0) 79 815 4318
> >
> > On Aug 26, 2013, at 21:54, "Carlos Costa"
> > <chncosta at gmail.com<mailto:chncosta at gmail.com>> wrote:
> >
> > Dear leishimmunologists,
> >
> > Do you have any guess on why splenectomy cures kala-azar? Would it be the
> > absence of spleen-derived IL-10?
> >
> > All my best,
> >
> > Carlos.
> >
> > --
> > Carlos H. N. Costa, MD, DSc.
> > Coordenador
> > Laborat?rio de Leishmanioses - LabLeish
> > Universidade Federal do Piau?
> > Instituto de Doen?as Tropicais Natan Portella
> > Rua Artur de Vasconcelos 151-Sul
> > 64001-450 Teresina-PI
> > Brazil
> > Telephones: +55 86 3222-4377 (W),
> > +55 86 3221-3062 (W),
> > +55 86 3237-1075 (R).
> >
> >
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> "Universidad Aut?noma de Yucat?n"
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>
> ------------------------------
>
> Message: 2
> Date: Fri, 30 Aug 2013 16:41:26 -0300
> From: Carlos Costa <chncosta at gmail.com>
> Subject: Re: [Leish-l] spleen
> To: Leish-L <leish-l at lineu.icb.usp.br>
> Message-ID:
> <
> CAJsAp4M_TBgWs6BRgd-Fs2Wp1k65wu0ivWuD8V8zvN_aBJo0pQ at mail.gmail.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> The idea of "amount of parasites", be it as the inoculation dose or as the
> spleen source of immunosuppression is very logical. However, as some have
> pointed, the different parasitized organs may have different roles on the
> control of the parasite, independently of the parasite itself, even as
> reversal cause, since the spleen may be the heaviest parasitized just due
> to its most inherent susceptibility.
>
> I wonder if bone marrow and spleen have different roles in kala-azar. For
> instances, although highly confounded by the effects of the disease, we do
> not know of bone marrow aplasia as a cure (or a cure helper) of kala-azar;
> just the contrary, as indicated by the title of this paper (that I wish
> somebody send it to me):
>
> Sirvent-von Bueltzingsloewen A, Marty P, Rosenthal E, Delaunay P,
> Allieri-Rosenthal A, Gratecos N, Cassuto JP. Visceral leishmaniasis: a new
> opportunistic infection in hematopoietic stem-cell-transplanted patients.
> Bone
> Marrow Transplant. 2004 Mar;33(6):667-8. PubMed PMID: 14730334.
>
> If this is real we might think of spleen as the immunossuppressor organ and
> bone marrow as the protector system?
>
> Paul Kaye and his team did a wonderful work years ago on a related subject
> (see attached).
>
> Nice weekend!
>
> Carlos.
>
>
>
>
> 2013/8/30 "Fernando Jos? Andrade Narv?ez" <anarvaez at uady.mx>
>
> > Dear lesihmaniacs,
> > I agree with all previous comments and suggestions. It is a very good
> > question but it is important to remark that the spleen is an organ of
> the
> > immune system and play different roles. In a recent paper, Chin Med J
> > (Engl). 2013 Jun;126(12):2354-60. Splenectomy protects experimental rats
> > from cerebral damage after stroke due to anti-inflammatory effects.
> > Zhang BJ, Men XJ, Lu ZQ, Li HY, Qiu W, Hu XQ, the authors discussed
> > antinflammatory effects of spelenctomy. Moreover the spleen is very
> > important in "humoral" antibodies-response which is increased in VL.
> > Another issue is to point out regarding the possibility of problems after
> > splenctomy described in the literature.
> > Best regards,
> > Fernando J. Andrade-Narvaez, MD, PhD
> > Chairman Bioethics Commission, State of Yucatan, Mexico,
> > Research Bioethics Committee Chairman
> > Head of Immunology Laboratory
> > Centro de Investigaciones Regionales "Dr. Hideyo Noguchi"
> > Universidad Aut?noma de Yucat?n,
> > Ave. Itz?es No. 490x59 CP 97000
> > M?rida, Yucat?n, M?xico
> > Tel.: 52 999 924-6412 ext. 1156
> > Fax: 52 999 923-6120
> > E-mails: anarvaez at uady.mx, fandrade47 at gmail.com
> >
> > > Dear Carlos,> Your idea of IL-10 is certainly a good guess but the
> > spleen is loaded with
> > > undifferentiated monocytic cells that can be infected by Leishmania but
> > > can not be activated to kill the parasites. We hypothesized and called
> > > these cells "Safe Targets" that are responsible for perpetuating
> > infection
> > > even after recovery from disease - based on mouse models.
> > > Do you think splenectomy cures or just reduces the parasite load,
> anemia
> > > and also IL-1 so patients feel better but would eventually relapse if
> not
> > > treated further?
> > > Best wishes
> > > Farrokh
> > >
> > > Sent from my DNDi iPhone
> > > +41(0) 79 815 4318
> > >
> > > On Aug 26, 2013, at 21:54, "Carlos Costa"
> > > <chncosta at gmail.com<mailto:chncosta at gmail.com>> wrote:
> > >
> > > Dear leishimmunologists,
> > >
> > > Do you have any guess on why splenectomy cures kala-azar? Would it be
> the
> > > absence of spleen-derived IL-10?
> > >
> > > All my best,
> > >
> > > Carlos.
> > >
> > > --
> > > Carlos H. N. Costa, MD, DSc.
> > > Coordenador
> > > Laborat?rio de Leishmanioses - LabLeish
> > > Universidade Federal do Piau?
> > > Instituto de Doen?as Tropicais Natan Portella
> > > Rua Artur de Vasconcelos 151-Sul
> > > 64001-450 Teresina-PI
> > > Brazil
> > > Telephones: +55 86 3222-4377 (W),
> > > +55 86 3221-3062 (W),
> > > +55 86 3237-1075 (R).
> > >
> > >
> > > Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS,
> > > protegidas pelo sigilo legal, por direitos autorais e destinadas
> > > exclusivamente ? pessoa ou organiza??o para a qual a mensagem foi
> > > destinada.
> > > Warning: This message is meant only for the intended recipient of the
> > > transmission. It is forbidden any unauthorized use, alteration,
> > > reproduction and distribution. If you are not the correct recipient,
> > > please notify us immediately by return e-mail and delete this message
> > from
> > > your system.
> > >
> > >
> > > --
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> >
> >
> >
> >
> > -----------------------------------------
> > "Universidad Aut?noma de Yucat?n"
> > http://www.uady.mx/
> >
> >
>
>
> --
> Carlos H. N. Costa, MD, DSc.
> Coordenador
> Laborat?rio de Leishmanioses - LabLeish
> Universidade Federal do Piau?
> Instituto de Doen?as Tropicais Natan Portella
> Rua Artur de Vasconcelos 151-Sul
> 64001-450 Teresina-PI
> Brazil
> Telephones: +55 86 3222-4377 (W),
> +55 86 3221-3062 (W),
> +55 86 3237-1075 (R).
>
>
> Aviso: As informa??es contidas nesta mensagem s?o CONFIDENCIAIS, protegidas
> pelo sigilo legal, por direitos autorais e destinadas exclusivamente ?
> pessoa ou organiza??o para a qual a mensagem foi destinada.
> Warning: This message is meant only for the intended recipient of the
> transmission. It is forbidden any unauthorized use, alteration,
> reproduction and distribution. If you are not the correct recipient, please
> notify us immediately by return e-mail and delete this message from your
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