<html>
<body>
<font size=3>In our paper from 1983 we found that for indirect
immunofluorescence.studies, a heterologous <i>L. donovani
</i>antigen gave higher titres than a homologous <i>L. major </i>antigen
for sera from vaccinated individuals. <br><br>
GREEN, M.S., KARK, J.D., GREENBLATT, C.L., LONDNER, M.V., FRANKENBURG,
S. and JACOBSON, R.,L. (1983). The cellular and humoral
immune response in subjects vaccinated against cutaneous leishmaniasis
using Leishmania tropica major promastigotes. Parasit.
Immunol. 5 337-344. <br><br>
<b>Summary</b> In vitro lymphocyte transformation was studied in 24
subjects 12 months after vaccination with live Leishmania tropica major
vaccine, in 10 normal control subjects and in three controls residing in
an endemic area. The vaccines had lesions in various stages of clinical
development. Lymphocytes from all the subjects were studied for their
response to stimulation with L. tropica major promastigotes. Lymphocytes
of vaccinated subjects responded to low concentrations of antigen whereas
the lymphocyte response of the controls tended to be depressed by the
same concentration of the antigen. Serum from each subject was subjected
to a study of the humoral antibody titre against L. tropica major and L.
donovani using indirect immunofluorescence. A humoral response to L.
donovani was present in a majority of vaccinees who had developed a
positive lesion whereas no such response was present in any of the
controls. The data suggest that high humoral responses were accompanied
by relatively low cell-mediated responses and vice versa. No significant
humoral response to L. tropica major could be demonstrated in any of the
subjects. A combination of both the cell-mediated and humoral mechanisms
may participate in the immune response although their usefulness in the
assessment of the protectivity of leishmania vaccines has not been
established.<br><br>
</font><font face="Tahoma" size=2>Jake Jacobson<br><br>
<br><br>
The accepted idea of no antibodies in cutaneous leishamaniasis is
incorrect. They definitely do occur in some but not all cases. However,
they may be missed if the wrong antigen is used. The greatest chance of
detecting them is to use the homologous antigen.<br>
Jeffrey Shaw <br>
</font><x-sigsep><p></x-sigsep>
<div align="center"><font size=3>Imagination was given to man to
compensate him for what he is not; a sense of humor to console him for
what he is.<br><br>
<br>
</div>
Dr.R.L.Jacobson MPH PhD<br>
Department of Molecular Genetics and Microbiology, <br>
The Institute of Medical Research Canada - Israel. <br>
The Kuvin Center For The Study Of Tropical and Infectious Diseases<br>
The Hebrew University-Hadassah Medical School<br>
POB 12272, Jerusalem, 91120<br>
Israel<br>
Telephone 972-2-6758077<br>
Fax<x-tab> </x-tab>
972-2-6757425<br>
Mobile 054-4970731<br>
</font><font size=3 color="#0000FF"><b>NEW:<br>
VOIP (from USA) 415-963-9801 (up to 15:00hrs
EST)</font></b></body>
</html>