[Leish-l] inquiry

LRU-Jericho islahjr at yahoo.com
Sat May 14 14:19:53 BRT 2011


Dear Saad,

If you have 20-30 cultures from various areas in Saudi Arabia, a good work on 
genetic clustering of leishmaniasis. The same applies for Lebanon.

Regards,
Amer





________________________________
From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br" 
<leish-l at lineu.icb.usp.br>
Sent: Fri, May 13, 2011 7:39:33 PM
Subject: Re: [Leish-l] inquiry

Dear Saad first precaution to take is to separate your samples into several 
portions each (at least 5 each being no more than a few hundred(200-400) 
microliters). This is a necessary precaution to avoid freezing and thawing 
several times the same sample which will be the case should you do different 
studies at different times which is unavoidable.

What you can do is correlate the type(ulcerated, abscess, furuncle etc.. ) the 
locale, the number and age of lesions with the levels of antibody in the sera of 
these patgients and also monitor cell mediated immunity with leishmanin skin 
test!
I have just published (in print) an article about having circulating parasites 
in some of these patients with apparently pure cutaneous disease. It is the 
first paper that revealed this fact. It would be interesting to confirm or 
de-confirm this fact repeating what we did.
Best wishes 

Nuha Nuwayri-Salti MD                                                            
                                                                                
                                                                                
                                                                       AOA 
Medical Honor Society                                                            
          

    

-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] 
On Behalf Of saad saad
Sent: Friday, April 29, 2011 11:23 PM
To: leish-l at lineu.icb.usp.br
Subject: [Leish-l] inquiry

Dear all
Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
CL from south west of the country. I would like to have your
recommendations to start a good research line in CL using these sera.
Thanks in advance for your help
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