[Leish-l] inquiry
LRU-Jericho
islahjr at yahoo.com
Sat May 14 14:19:53 BRT 2011
Dear Saad,
If you have 20-30 cultures from various areas in Saudi Arabia, a good work on
genetic clustering of leishmaniasis. The same applies for Lebanon.
Regards,
Amer
________________________________
From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
<leish-l at lineu.icb.usp.br>
Sent: Fri, May 13, 2011 7:39:33 PM
Subject: Re: [Leish-l] inquiry
Dear Saad first precaution to take is to separate your samples into several
portions each (at least 5 each being no more than a few hundred(200-400)
microliters). This is a necessary precaution to avoid freezing and thawing
several times the same sample which will be the case should you do different
studies at different times which is unavoidable.
What you can do is correlate the type(ulcerated, abscess, furuncle etc.. ) the
locale, the number and age of lesions with the levels of antibody in the sera of
these patgients and also monitor cell mediated immunity with leishmanin skin
test!
I have just published (in print) an article about having circulating parasites
in some of these patients with apparently pure cutaneous disease. It is the
first paper that revealed this fact. It would be interesting to confirm or
de-confirm this fact repeating what we did.
Best wishes
Nuha Nuwayri-Salti MD
AOA
Medical Honor Society
-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br]
On Behalf Of saad saad
Sent: Friday, April 29, 2011 11:23 PM
To: leish-l at lineu.icb.usp.br
Subject: [Leish-l] inquiry
Dear all
Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
CL from south west of the country. I would like to have your
recommendations to start a good research line in CL using these sera.
Thanks in advance for your help
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