<html><head><style type="text/css"><!-- DIV {margin:0px;} --></style></head><body><div style="font-family:arial,helvetica,sans-serif;font-size:12pt"><div style="font-family:arial, helvetica, sans-serif;font-size:12pt">Dear Saad,<br><br>If you have 20-30 cultures from various areas in Saudi Arabia, a good work on genetic clustering of leishmaniasis. The same applies for Lebanon.<br><br>Regards,<br>Amer<br><br><br><br><div style="font-family:arial, helvetica, sans-serif;font-size:10pt"><font size="2" face="Tahoma"><hr size="1"><b><span style="font-weight: bold;">From:</span></b> Nuha Nuwayri-Salti <racha@aub.edu.lb><br><b><span style="font-weight: bold;">To:</span></b> saad saad <saad1426@gmail.com>; "leish-l@lineu.icb.usp.br" <leish-l@lineu.icb.usp.br><br><b><span style="font-weight: bold;">Sent:</span></b> Fri, May 13, 2011 7:39:33 PM<br><b><span style="font-weight: bold;">Subject:</span></b> Re: [Leish-l] inquiry<br></font><br>
Dear Saad first precaution to take is to separate your samples into several portions each (at least 5 each being no more than a few hundred(200-400) microliters). This is a necessary precaution to avoid freezing and thawing several times the same sample which will be the case should you do different studies at different times which is unavoidable.<br><br>What you can do is correlate the type(ulcerated, abscess, furuncle etc.. ) the locale, the number and age of lesions with the levels of antibody in the sera of these patgients and also monitor cell mediated immunity with leishmanin skin test!<br>I have just published (in print) an article about having circulating parasites in some of these patients with apparently pure cutaneous disease. It is the first paper that revealed this fact. It would be interesting to confirm or de-confirm this fact repeating what we did.<br>Best wishes <br><br>Nuha Nuwayri-Salti MD
AOA Medical Honor Society <br> <br><br>-----Original Message-----<br>From: <a ymailto="mailto:leish-l-bounces@lineu.icb.usp.br" href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> [mailto:<a ymailto="mailto:leish-l-bounces@lineu.icb.usp.br" href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>] On Behalf Of saad saad<br>Sent: Friday, April 29, 2011 11:23 PM<br>To: <a ymailto="mailto:leish-l@lineu.icb.usp.br" href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>Subject: [Leish-l] inquiry<br><br>Dear all<br>Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of<br>CL from south west of the country. I
would like to have your<br>recommendations to start a good research line in CL using these sera.<br>Thanks in advance for your help<br>_______________________________________________<br>Leish-l mailing list<br><a ymailto="mailto:Leish-l@lineu.icb.usp.br" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br><span><a target="_blank" href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a></span><br>_______________________________________________<br>Leish-l mailing list<br><a ymailto="mailto:Leish-l@lineu.icb.usp.br" href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" target="_blank">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br></div></div>
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