[Leish-l] inquiry

[Nuha Nuwayri-Salti]

Dear Saad first precaution to take is to separate your samples into several portions each (at least 5 each being no more than a few hundred(200-400) microliters). This is a necessary precaution to avoid freezing and thawing several times the same sample which will be the case should you do different studies at different times which is unavoidable.

What you can do is correlate the type(ulcerated, abscess, furuncle etc.. ) the locale, the number and age of lesions with the levels of antibody in the sera of these patgients and also monitor cell mediated immunity with leishmanin skin test!
I have just published (in print) an article about having circulating parasites in some of these patients with apparently pure cutaneous disease. It is the first paper that revealed this fact. It would be interesting to confirm or de-confirm this fact repeating what we did.
Best wishes 

Nuha Nuwayri-Salti MD                                                                                                                                                                                                                                                                                                   AOA Medical Honor Society                                                                       
    

-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of saad saad
Sent: Friday, April 29, 2011 11:23 PM
To: leish-l at lineu.icb.usp.br
Subject: [Leish-l] inquiry

Dear all
Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
CL from south west of the country. I would like to have your
recommendations to start a good research line in CL using these sera.
Thanks in advance for your help
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