[Leish-l] Confirm hits to Leishmania spp. in whole blood of mammals in the US?

[Rodrigo Leite]

Dear Sayler, It's possible to detect Leishmania DNA in the reservoir host.
But, it is necessary to define what will be the reservior host to be evaluated.
For exemple, blood dog is bad sample for PCR.

Rodrigo Leite


2015-03-05 20:30 GMT-03:00 Sayler,Katherine A <saylerk at ufl.edu>:

>  Dear readers,
>
>
>  Thank you sincerely for your time. I am a tick-borne disease biologist
> by trade, so this is outside of my realm.
>
>
>  I am trying to confirm some preliminary data that suggests the
> *Leishmania* spp. are circulating in limited geographic locales in
> southern Florida. Is it at all reasonable to expect to detect the DNA of
> the trypanosomatids in the blood of infected wildlife? My suspicion is that
> this is completely improbable, as it appears that in order to detect DNA in
> enough quantity or quality for sequencing, you need cultured organisms.
> From sandflies it appears that it is possible to PCR amplify and sequence
> directly from the vector, but never from the reservoir (or potential
> reservoir) host. Is this true? Any advice or insight would be greatly
> appreciated and I am tremendously naïve and this is clearly not my area of
> expertise.
>
>
>  Thank you again for reading my message,
>
> Katherine
>
>  Katherine Sayler, M.Ed., Ph.D.
> Postdoctoral Associate | Department of Wildlife Ecology and Conservation
> Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611
> Phone: 352-871-3259
>
>
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