<div dir="ltr"><div>Dear Sayler, It's possible to detect Leishmania DNA in <font size="3" face="Calibri"><span style="font-size:12pt"><span dir="ltr"><font size="3"><span style="background-color:white">the reservoir host. But, </span></font></span></span></font><span id="result_box" class="" lang="en"><span class="">it is necessary to</span> <span class="">define</span> <span class="">what will be the</span> reservior <span class="">host to be</span> <span class="">evaluated. For exemple, blood dog is bad sample for PCR. <br><br></span></span></div><span id="result_box" class="" lang="en"><span class="">Rodrigo Leite<br><br></span></span></div><div class="gmail_extra"><br><div class="gmail_quote">2015-03-05 20:30 GMT-03:00 Sayler,Katherine A <span dir="ltr"><<a href="mailto:saylerk@ufl.edu" target="_blank">saylerk@ufl.edu</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p>Dear readers, </p>
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<p>Thank you sincerely for your time. I am a tick-borne disease biologist by trade, so this is outside of my realm.
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<p><font size="3" face="Calibri"><span style="font-size:12pt"><span dir="ltr"><span style="background-color:white">I am trying to confirm some preliminary data that suggests the
<em>Leishmania</em> spp. are circulating in limited geographic locales in southern Florida. </span><font size="3"><span style="background-color:white">Is it at all reasonable to expect to detect the DNA of the trypanosomatids in the blood of infected wildlife?
My suspicion is that this is completely improbable, as it appears that in order to detect DNA in enough quantity or quality for sequencing, you need cultured organisms. From sandflies it appears that it is possible to PCR amplify and sequence directly from
the vector, but never from the reservoir (or potential reservoir) host. Is this true? Any advice or insight would be greatly appreciated and I am tremendously naïve and this is clearly not my area of expertise</span></font></span></span></font>.<br>
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<p>Thank you again for reading my message,</p>
<p>Katherine<br>
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<div><font face="Arial">Katherine Sayler, M.Ed., Ph.D.</font></div>
<div><font face="Arial">Postdoctoral Associate | Department of Wildlife Ecology and Conservation</font></div>
<div><font face="Arial">Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611</font></div>
<div><font face="Arial">Phone: <a href="tel:352-871-3259" value="+13528713259" target="_blank">352-871-3259</a> </font></div>
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