[Leish-l] [Possivel Spam] Re: Confirm hits to Leishmania spp. in whole blood of mammals in the US?

[Patrick Bastien]

Dear colleague,
It is certainly highly feasible to detect Leishmania in any mammalian  
reservoir. Indeed, as compared to many other microorganisms,  
Leishmania benefits from a highly repetitive DNA target termed the  
'minicircle' of the kinetoplast (mitochondrial DNA). This allows an  
extremely sensitive PCR detection, hence detecting very low parasitic  
loads, in particular in the blood or in other organs/tissues (see Mary  
et al., Lachaud et al. and many others).
In contrast, this extreme sensitivity also leads to high rates of  
carry-over contaminations of the PCR which oblige to extreme  
precautions in the setting up of the PCR environment and realization.
Other, less repetitive, DNA targets may be used, such as the rDNA.  
They offer a more robust but clearly less sensitive detection.
See PubMed for numerous publications in dogs and humans, incl. from  
the USA, using a variety of DNA targets and PCR assays.
Regards
P. Bastien

Pr Patrick Bastien
Director, Departement de Parasitologie-Mycologie
Centre National de Reference des Leishmanioses
Vice-Director, "MiVEGEC" unit research UMR5290 CNRS/224 IRD/UM1/UM2
Faculte de Medecine, Université Montpellier 1
39 Av. Charles Flahault
34295 Montpellier cedex 5


----- Message de parasito at univ-montp1.fr ---------
     Date : Fri, 06 Mar 2015 10:31:17 +0100
      De : "Dedet Jean-Pierre: <parasito at univ-montp1.fr>"  
<parasito at univ-montp1.fr>
Répondre à : "Dedet Jean-Pierre: <parasito at univ-montp1.fr>"  
<parasito at univ-montp1.fr>
  Objet : Trans.: [Leish-l] Confirm hits to Leishmania spp. in whole  
blood of mammals in the US?
       À : Patrick Bastien <patrick.bastien at univ-montp1.fr>
      Cc : f-pratlong at chu-montpellier.fr, laurence lachaud <llachaud at gmail.com>


>
>
> ----- Message transféré de saylerk at ufl.edu -----
>     Date : Thu, 5 Mar 2015 23:30:21 +0000
>      De : "Sayler,Katherine A" <saylerk at ufl.edu>
> Répondre à : "Sayler,Katherine A" <saylerk at ufl.edu>
>  Objet : [Leish-l] Confirm hits to Leishmania spp. in whole blood of  
>  mammals in the US?
>       À : "leish-l at lineu.icb.usp.br" <leish-l at lineu.icb.usp.br>
>
> Dear readers,
>
>
> Thank you sincerely for your time. I am a tick-borne disease biologist
> by trade, so this is outside of my realm.
>
>
> I am trying to confirm some preliminary data that suggests the   
> Leishmania spp. are circulating in limited geographic locales in   
> southern Florida. Is it at all reasonable to expect to detect the  
> DNA  of the trypanosomatids in the blood of infected wildlife? My  
> suspicion  is that this is completely improbable, as it appears that  
> in order to  detect DNA in enough quantity or quality for  
> sequencing, you need  cultured organisms. From sandflies it appears  
> that it is possible to  PCR amplify and sequence directly from the  
> vector, but never from the  reservoir (or potential reservoir) host.  
> Is this true? Any advice or  insight would be greatly appreciated  
> and I am tremendously naïve and  this is clearly not my area of  
> expertise.
>
>
> Thank you again for reading my message,
>
> Katherine
>
> Katherine Sayler, M.Ed., Ph.D.
> Postdoctoral Associate | Department of Wildlife Ecology and Conservation
> Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611
> Phone: 352-871-3259
>
> --
> This email was sent by icb.usp.br
>
>
> ----- Fin du message transféré -----
>
>
> _______________________________
> Professeur Jean-Pierre Dedet
> Professeur émérite à la Faculté de Médecine
> Université de Montpellier
> UMR MIVEGEC (CNRS 5290-IRD 224-UM1-UM2)
> (Dir. F. Simard)
>
> Adresse postale :
> Laboratoire de Parasitologie
> 39, Avenue Charles Flahault
> 34295 MONTPELLIER CEDEX 5
> FRANCE
>
> tel. : 33 (0)4 67 33 23 50
> fax :  33 (0)4 67 33 23 58
> http://www.parasitologie.univ-**montp1.fr<<http://www.parasitologie.univ-
> />http://www.parasitologie.univ-
> <http://montp1.fr/>montp1.fr>


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