[Leish-l] Leish-l Digest, Vol 72, Issue 1
Ayman El-Badry
aelbadry at kasralainy.edu.eg
Fri Jan 10 07:15:13 BRST 2014
Dear All
kindly we would like to collect blood for molecular detection of visceral
leishmania from village 500 Km away from the lab
is there a method to keep the sample intact tell reaching the lab on same
day or may we have 24-48 hours delay
is there any one have experience and SOP with percoll, ficoll or histopque
could a buffy coat separated at field stand a time (24-48 hours) at room
temperature without affecting Leishmania DNA or RNA extraction
best regards
On Fri, Jan 10, 2014 at 9:49 AM, <leish-l-request at lineu.icb.usp.br> wrote:
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> Today's Topics:
>
> 1. Induction of LST positivity by BCG vaccination (Farrokh Modabber)
> 2. Re: why is Leishmania donovani restricted to humans in Indian
> subcontinent? (?????????? ?????)
> 3. Re: etanercept and kala-azar (jos? carlos)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 2 Dec 2013 13:29:20 +0000
> From: Farrokh Modabber <fmodabber at dndi.org>
> Subject: [Leish-l] Induction of LST positivity by BCG vaccination
> To: "Leish-l at lineu.icb.usp.br" <Leish-l at lineu.icb.usp.br>
> Message-ID:
> <5EA2E08A390CC64989B542B01E4F31F33E890D92 at Exchmb2.DNDI-ORG.LOCAL>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear Colleagues,
> I recall from Prof. Mayrinks studies, that BCG vaccination may convert
> leishmanin skin test in some volunteers who are LST-negative
> (MST-negative). The reactivity would gradually diminish after a few months.
> Would appreciate any information on that and on Prof. Mayrink's current
> email address.
> Many thanks,
> Farrokh Modabber
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> ------------------------------
>
> Message: 2
> Date: Mon, 2 Dec 2013 16:36:24 +0200
> From: ?????????? ????? <ksoteriadou at pasteur.gr>
> Subject: Re: [Leish-l] why is Leishmania donovani restricted to humans
> in Indian subcontinent?
> To: "Isabel Mauricio" <isabel.mauricio at ihmt.unl.pt>,
> <Leish-L at lineu.icb.usp.br>
> Message-ID:
> <A9356D2B2403EF4FA096C4B5961F650D03D63711 at srv-mailii.pasteur.gr>
> Content-Type: text/plain; charset="iso-8859-7"
>
> Well done Isabel!!!!
> Ketty/Kaiti
>
> ________________________________
>
> ???: leish-l-bounces at lineu.icb.usp.br ?? ?????? Isabel Mauricio
> ????????: ??? 15/11/2013 16:13
> ????: Leish-L at lineu.icb.usp.br
> ????: Re: [Leish-l] why is Leishmania donovani restricted to humans in
> Indian subcontinent?
>
>
>
> Dear all
>
>
>
> I don't think it is as simple as saying that there was an introduction of
> L. donovani in India from East Africa or vice-versa. Genetically, the two
> are quite distinct, and although the Indian focus is quite homogeneous,
> phylogenetically it doesn't emerge from the East African strains.
>
> It is more likely that they have evolved separately. Older populations may
> have become extinct, and it's not clear what is the origin of the Indian
> (actually East Indian) strains (MON-2). Their ancestral populations may
> well have disappeared. The surviving branch could have been the best
> adapted to the local sand fly population.
>
>
>
> In any case, when talking about East Africa, one must distinguish between
> the Sudan/Ethiopia region and the Kenyan region. They are genetically
> distinct, as well, and do not form a homogeneous group.
>
>
>
> A PNAS paper that analysed a large amount of genetic marker data for
> selected strains suggests that the Indian strains belong to an early
> branching group from a larger population that migrated through central Asia.
>
>
>
> http://www.pnas.org/content/104/22/9375.full.pdf <
> http://www.pnas.org/content/104/22/9375.full.pdf>
>
>
>
> All the best
>
> Isabel Mauricio
>
>
>
> De: Kandan Ilango [mailto:kilangozsi at gmail.com]
> Enviada: 13 de novembro de 2013 05:45
> Para: Lawyer, Phillip (NIH/NIAID) [E]
> Cc: Leish-L at lineu.icb.usp.br
> Assunto: Re: [Leish-l] why is Leishmania donovani restricted to humans in
> Indian subcontinent?
>
>
>
> Dear all,
>
>
>
> Phil comment tempts me to respond on the origin of L. donvani from India
> to E. Africa. I think Carlos remarks in some sense was right because
> co-cladogensis events of L. donvani sensu lato and its associated vectors
> ie. the members of P. (Euphlebotomus) argentipes in India and P.
> (Symphlebotomus) martini + others in East Africa. Although the exact age of
> separation of Euphlebotomus from Anaphlebotomus (visa-vice) together with
> their Gondwanaland and Afrotropical continents remains speculative but the
> recent publication (of El-naiem et at (2011): A possible role for
> Phlebotomus (Anaphlebotomus) rodhaini (Parrot, 1930) in transmission of
>
> Leishmania donovani) attributes that host shift could be a potential
> factor with respect to infection of L. donvani in P.martini + others and P.
> rodhaini.
>
>
>
> With best wishes,
>
> K. Ilango.
>
>
>
>
> Dr K. Ilango, M Sc, PhD (London), Dip LSHTM
>
> Dy. Director & Officer-in-Charge
>
> Zoological Survey of India
> (Government of India)
>
> Southern Regional Centre
> 130 Santhome High Road
>
> Chennai-600 028, INDIA
>
> Phone/Fax No. +91 (0) 44 24642898
>
> Alternate e-mail: ilangokaniyan at yahoo.co.in <mailto:ilangokaniyan at yahoo.in
> >
>
>
>
> On Mon, Sep 16, 2013 at 9:07 AM, Lawyer, Phillip (NIH/NIAID) [E] <
> PhillipL at niaid.nih.gov> wrote:
>
> Dear Carlos,
>
>
>
> For what it's worth, I believe it most likely happened the other
> way around: Leishmania donovani was probably introduced to East Africa
> from India during the late 1800s when laborers were brought from India to
> work in the Kenya building the railroad from Mombasa to Uganda and on other
> infrastructure projects. Leishmania donovani in East Africa is manifest as
> kala azar and is anthroponotic, the same as in India. The main vector in
> Kenya is Phlebotmus martini, which tends to breed in termite mounds, often
> associated with human dwellings. Other Symphlebotomus species, Ph.
> vansomerenae and Ph. celiae have also been implicated in L. donovani
> transmission.
>
>
>
> Regards,
>
>
>
> Phil Lawyer
>
> ________________________________
> From: Carlos Brisola Marcondes [cbrisolamarcondes at gmail.com]
> Sent: Friday, August 30, 2013 8:41 AM
> To: Leish-L; leish-l-bounces at lineu.icb.usp.br
> Subject: [Leish-l] why is Leishmania donovani restricted to humans
> in Indian subcontinent?
>
> Dear all,
> Leishmania donovani seems to have been introduced from East Africa
> to Indian subcontinent, where it has infected mostly humans, differently
> from East African foci.
> Why does this occur? Is this caused by feeding preferences of
> Phlebotomus argentipes, which bites mostly ruminants and humans and is
> associated to houses? Or are dogs rarer in that region than in Brazil,
> where these animals are important reservoirs of Leishmania infantum and
> frequently bitten by Lutzomyia longipalpis?
>
>
> Sincerely yours
> prof. dr. Carlos Brisola Marcondes
> Dept. Microbiol. Imunol. Parasitol./CCB
> Federal University of Santa Catarina
> Florianopolis (SC)
> CV:
> http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2
> blog: http://entomomedica.blogspot.com.br/
>
>
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> ------------------------------
>
> Message: 3
> Date: Mon, 02 Dec 2013 09:57:05 -0300
> From: jos? carlos <jcribeiro.pi at ig.com.br>
> Subject: Re: [Leish-l] etanercept and kala-azar
> To: leish-l at lineu.icb.usp.br, Michal Baniyash
> <michalb at ekmd.huji.ac.il>, Carlos Costa <chncosta at gmail.com>
> Message-ID: <529C83A1.80107 at ig.com.br>
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>
> Em 28/11/2013 15:50, jos? carlos escreveu:
> > Good afternoon friends,
> >
> > I came across the attached article and was wondering if the use of
> > etanercept at the appropriate time would represent an important
> > advance in the treatment of kala-azar to allow the body does not come
> > into immunosuppression and destroy the parasite. I did a quick search
> > on pubmed and found no article that answered my question, MDSC are
> > elevated in leishmaniasis? etanercept at the appropriate time heals
> > the patient with kala-azar?
> >
> > best regards,
> >
> > Jos? Carlos
> > Lableish- IDTNP
> > Teresina - PI - Brazil
> dear friends,
>
> I received an email from michal dr baniyash (author of the article
> attached in previous mail) and everything indicates that MDSC are
> elevated in visceral leishmaniasis. after receiving his mail had a
> insigth that TNF-alpha increases and then to be deposited in the
> membranes of macrophages in the blood decreases, so if we use etanercept
> at the right time when the TNF-alpha is not yet deposited in the
> membranes can decrease immunosuppression. or will the TNF-alpha is not
> deposited, but is expressed by the macrophage itself inside out?
>
> regards,
>
> jos? carlos
>
>
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--
*Ayman*
Ayman A. El-Badry, M.D.
Prof of Medical Parasitology
Head of Diagnostic & research Unit of Parasitic Diseases (DRUP)
Medical Parasitology Dept., kasr Al-Ainy School of Medicine, Cairo
University
Postal Code 11559. POB: 109 El-Malek El Saleh Post Office - El Manial -
Cairo - Egypt.
Tel.: (+202) 23632733.
Fax: (+202) 23644383.
Mobile phone: (+2)
E-mail: <aelbadry at kasralainy.edu.eg>aelbadry at kasralainy.edu.eg<aelbadry at kasralainy.edu.eg,aymanbb at hotmail.com,http://facebook.com/aymanbb><aelbadry at kasralainy.edu.eg,aymanbb at hotmail.com,http://facebook.com/aymanbb>
,aymanbb at hotmail.com
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