[Leish-l] collect peripheral blood sample from field for molecular detection of VL

[Ayman El-Badry]

Dear All
kindly we would like to collect blood for molecular detection of visceral
leishmania from village 500 Km away from the lab
is there a method to keep the sample intact tell reaching the lab on same
day or may we have 24-48 hours delay
is there any one have experience and SOP with percoll, ficoll or histopque
could a buffy coat separated at field stand a time (24-48 hours) at room
temperature without affecting Leishmania DNA or RNA extraction

best regards

*Ayman*

Ayman A. El-Badry, M.D.
Prof of Medical Parasitology

Head of Diagnostic & research Unit of Parasitic Diseases (DRUP)

Medical Parasitology Dept., kasr Al-Ainy School of Medicine, Cairo
University

Postal Code 11559. POB: 109 El-Malek El Saleh Post Office - El Manial -
Cairo - Egypt.

Tel.: (+202) 23632733.

Fax: (+202) 23644383.

Mobile phone: (+2)

E-mail:  <aelbadry at kasralainy.edu.eg>aelbadry at kasralainy.edu.eg<aelbadry at kasralainy.edu.eg,aymanbb at hotmail.com,http://facebook.com/aymanbb><aelbadry at kasralainy.edu.eg,aymanbb at hotmail.com,http://facebook.com/aymanbb>
,aymanbb at hotmail.com

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