<div dir="ltr"><div class="gmail_extra" style="font-family:arial,sans-serif;font-size:12.800000190734863px">Dear All<div>kindly we would like to collect blood for molecular detection of visceral leishmania from village 500 Km away from the lab</div>
<div>is there a method to keep the sample intact tell reaching the lab on same day or may we have 24-48 hours delay</div><div>is there any one have experience and SOP with percoll, ficoll or histopque</div>could a buffy coat separated at field stand a time (24-48 hours) at room temperature without affecting Leishmania DNA or RNA extraction</div>
<div class="gmail_extra" style="font-family:arial,sans-serif;font-size:12.800000190734863px"><br></div><div class="gmail_extra" style="font-family:arial,sans-serif;font-size:12.800000190734863px">best regards </div></div>
<div class="gmail_extra"><br><br><div class="gmail_quote">On Fri, Jan 10, 2014 at 9:49 AM, <span dir="ltr"><<a href="mailto:leish-l-request@lineu.icb.usp.br" target="_blank">leish-l-request@lineu.icb.usp.br</a>></span> wrote:<br>
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Today's Topics:<br>
<br>
1. Induction of LST positivity by BCG vaccination (Farrokh Modabber)<br>
2. Re: why is Leishmania donovani restricted to humans in Indian<br>
subcontinent? (?????????? ?????)<br>
3. Re: etanercept and kala-azar (jos? carlos)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Mon, 2 Dec 2013 13:29:20 +0000<br>
From: Farrokh Modabber <<a href="mailto:fmodabber@dndi.org">fmodabber@dndi.org</a>><br>
Subject: [Leish-l] Induction of LST positivity by BCG vaccination<br>
To: "<a href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a>" <<a href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a>><br>
Message-ID:<br>
<5EA2E08A390CC64989B542B01E4F31F33E890D92@Exchmb2.DNDI-ORG.LOCAL><br>
Content-Type: text/plain; charset="us-ascii"<br>
<br>
Dear Colleagues,<br>
I recall from Prof. Mayrinks studies, that BCG vaccination may convert leishmanin skin test in some volunteers who are LST-negative (MST-negative). The reactivity would gradually diminish after a few months. Would appreciate any information on that and on Prof. Mayrink's current email address.<br>
Many thanks,<br>
Farrokh Modabber<br>
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Message: 2<br>
Date: Mon, 2 Dec 2013 16:36:24 +0200<br>
From: ?????????? ????? <<a href="mailto:ksoteriadou@pasteur.gr">ksoteriadou@pasteur.gr</a>><br>
Subject: Re: [Leish-l] why is Leishmania donovani restricted to humans<br>
in Indian subcontinent?<br>
To: "Isabel Mauricio" <<a href="mailto:isabel.mauricio@ihmt.unl.pt">isabel.mauricio@ihmt.unl.pt</a>>,<br>
<<a href="mailto:Leish-L@lineu.icb.usp.br">Leish-L@lineu.icb.usp.br</a>><br>
Message-ID:<br>
<<a href="mailto:A9356D2B2403EF4FA096C4B5961F650D03D63711@srv-mailii.pasteur.gr">A9356D2B2403EF4FA096C4B5961F650D03D63711@srv-mailii.pasteur.gr</a>><br>
Content-Type: text/plain; charset="iso-8859-7"<br>
<br>
Well done Isabel!!!!<br>
Ketty/Kaiti<br>
<br>
________________________________<br>
<br>
???: <a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> ?? ?????? Isabel Mauricio<br>
????????: ??? 15/11/2013 16:13<br>
????: <a href="mailto:Leish-L@lineu.icb.usp.br">Leish-L@lineu.icb.usp.br</a><br>
????: Re: [Leish-l] why is Leishmania donovani restricted to humans in Indian subcontinent?<br>
<br>
<br>
<br>
Dear all<br>
<br>
<br>
<br>
I don't think it is as simple as saying that there was an introduction of L. donovani in India from East Africa or vice-versa. Genetically, the two are quite distinct, and although the Indian focus is quite homogeneous, phylogenetically it doesn't emerge from the East African strains.<br>
<br>
It is more likely that they have evolved separately. Older populations may have become extinct, and it's not clear what is the origin of the Indian (actually East Indian) strains (MON-2). Their ancestral populations may well have disappeared. The surviving branch could have been the best adapted to the local sand fly population.<br>
<br>
<br>
<br>
In any case, when talking about East Africa, one must distinguish between the Sudan/Ethiopia region and the Kenyan region. They are genetically distinct, as well, and do not form a homogeneous group.<br>
<br>
<br>
<br>
A PNAS paper that analysed a large amount of genetic marker data for selected strains suggests that the Indian strains belong to an early branching group from a larger population that migrated through central Asia.<br>
<br>
<br>
<br>
<a href="http://www.pnas.org/content/104/22/9375.full.pdf" target="_blank">http://www.pnas.org/content/104/22/9375.full.pdf</a> <<a href="http://www.pnas.org/content/104/22/9375.full.pdf" target="_blank">http://www.pnas.org/content/104/22/9375.full.pdf</a>><br>
<br>
<br>
<br>
All the best<br>
<br>
Isabel Mauricio<br>
<br>
<br>
<br>
De: Kandan Ilango [mailto:<a href="mailto:kilangozsi@gmail.com">kilangozsi@gmail.com</a>]<br>
Enviada: 13 de novembro de 2013 05:45<br>
Para: Lawyer, Phillip (NIH/NIAID) [E]<br>
Cc: <a href="mailto:Leish-L@lineu.icb.usp.br">Leish-L@lineu.icb.usp.br</a><br>
Assunto: Re: [Leish-l] why is Leishmania donovani restricted to humans in Indian subcontinent?<br>
<br>
<br>
<br>
Dear all,<br>
<br>
<br>
<br>
Phil comment tempts me to respond on the origin of L. donvani from India to E. Africa. I think Carlos remarks in some sense was right because co-cladogensis events of L. donvani sensu lato and its associated vectors ie. the members of P. (Euphlebotomus) argentipes in India and P. (Symphlebotomus) martini + others in East Africa. Although the exact age of separation of Euphlebotomus from Anaphlebotomus (visa-vice) together with their Gondwanaland and Afrotropical continents remains speculative but the recent publication (of El-naiem et at (2011): A possible role for Phlebotomus (Anaphlebotomus) rodhaini (Parrot, 1930) in transmission of<br>
<br>
Leishmania donovani) attributes that host shift could be a potential factor with respect to infection of L. donvani in P.martini + others and P. rodhaini.<br>
<br>
<br>
<br>
With best wishes,<br>
<br>
K. Ilango.<br>
<br>
<br>
<br>
<br>
Dr K. Ilango, M Sc, PhD (London), Dip LSHTM<br>
<br>
Dy. Director & Officer-in-Charge<br>
<br>
Zoological Survey of India<br>
(Government of India)<br>
<br>
Southern Regional Centre<br>
130 Santhome High Road<br>
<br>
Chennai-600 028, INDIA<br>
<br>
Phone/Fax No. +91 (0) 44 24642898<br>
<br>
Alternate e-mail: <a href="mailto:ilangokaniyan@yahoo.co.in">ilangokaniyan@yahoo.co.in</a> <mailto:<a href="mailto:ilangokaniyan@yahoo.in">ilangokaniyan@yahoo.in</a>><br>
<br>
<br>
<br>
On Mon, Sep 16, 2013 at 9:07 AM, Lawyer, Phillip (NIH/NIAID) [E] <<a href="mailto:PhillipL@niaid.nih.gov">PhillipL@niaid.nih.gov</a>> wrote:<br>
<br>
Dear Carlos,<br>
<br>
<br>
<br>
For what it's worth, I believe it most likely happened the other way around: Leishmania donovani was probably introduced to East Africa from India during the late 1800s when laborers were brought from India to work in the Kenya building the railroad from Mombasa to Uganda and on other infrastructure projects. Leishmania donovani in East Africa is manifest as kala azar and is anthroponotic, the same as in India. The main vector in Kenya is Phlebotmus martini, which tends to breed in termite mounds, often associated with human dwellings. Other Symphlebotomus species, Ph. vansomerenae and Ph. celiae have also been implicated in L. donovani transmission.<br>
<br>
<br>
<br>
Regards,<br>
<br>
<br>
<br>
Phil Lawyer<br>
<br>
________________________________<br>
From: Carlos Brisola Marcondes [<a href="mailto:cbrisolamarcondes@gmail.com">cbrisolamarcondes@gmail.com</a>]<br>
Sent: Friday, August 30, 2013 8:41 AM<br>
To: Leish-L; <a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a><br>
Subject: [Leish-l] why is Leishmania donovani restricted to humans in Indian subcontinent?<br>
<br>
Dear all,<br>
Leishmania donovani seems to have been introduced from East Africa to Indian subcontinent, where it has infected mostly humans, differently from East African foci.<br>
Why does this occur? Is this caused by feeding preferences of Phlebotomus argentipes, which bites mostly ruminants and humans and is associated to houses? Or are dogs rarer in that region than in Brazil, where these animals are important reservoirs of Leishmania infantum and frequently bitten by Lutzomyia longipalpis?<br>
<br>
<br>
Sincerely yours<br>
prof. dr. Carlos Brisola Marcondes<br>
Dept. Microbiol. Imunol. Parasitol./CCB<br>
Federal University of Santa Catarina<br>
Florianopolis (SC)<br>
CV: <a href="http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2" target="_blank">http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2</a><br>
blog: <a href="http://entomomedica.blogspot.com.br/" target="_blank">http://entomomedica.blogspot.com.br/</a><br>
<br>
<br>
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Message: 3<br>
Date: Mon, 02 Dec 2013 09:57:05 -0300<br>
From: jos? carlos <<a href="mailto:jcribeiro.pi@ig.com.br">jcribeiro.pi@ig.com.br</a>><br>
Subject: Re: [Leish-l] etanercept and kala-azar<br>
To: <a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>, Michal Baniyash<br>
<<a href="mailto:michalb@ekmd.huji.ac.il">michalb@ekmd.huji.ac.il</a>>, Carlos Costa <<a href="mailto:chncosta@gmail.com">chncosta@gmail.com</a>><br>
Message-ID: <<a href="mailto:529C83A1.80107@ig.com.br">529C83A1.80107@ig.com.br</a>><br>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed<br>
<br>
Em 28/11/2013 15:50, jos? carlos escreveu:<br>
> Good afternoon friends,<br>
><br>
> I came across the attached article and was wondering if the use of<br>
> etanercept at the appropriate time would represent an important<br>
> advance in the treatment of kala-azar to allow the body does not come<br>
> into immunosuppression and destroy the parasite. I did a quick search<br>
> on pubmed and found no article that answered my question, MDSC are<br>
> elevated in leishmaniasis? etanercept at the appropriate time heals<br>
> the patient with kala-azar?<br>
><br>
> best regards,<br>
><br>
> Jos? Carlos<br>
> Lableish- IDTNP<br>
> Teresina - PI - Brazil<br>
dear friends,<br>
<br>
I received an email from michal dr baniyash (author of the article<br>
attached in previous mail) and everything indicates that MDSC are<br>
elevated in visceral leishmaniasis. after receiving his mail had a<br>
insigth that TNF-alpha increases and then to be deposited in the<br>
membranes of macrophages in the blood decreases, so if we use etanercept<br>
at the right time when the TNF-alpha is not yet deposited in the<br>
membranes can decrease immunosuppression. or will the TNF-alpha is not<br>
deposited, but is expressed by the macrophage itself inside out?<br>
<br>
regards,<br>
<br>
jos? carlos<br>
<br>
<br>
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</blockquote></div><br><br clear="all"><div><br></div>-- <br><div dir="ltr"><font size="3" face="Times New Roman">
</font><p style="margin:0cm 0cm 0pt;text-align:left;line-height:normal;direction:ltr"><strong><span style="color:rgb(79,129,189);font-size:10pt"><font face="Calibri">Ayman</font></span></strong></p><font size="3" face="Times New Roman">
</font><div style="margin:0cm 0cm 0pt;text-align:left;line-height:normal;direction:ltr"><span style="color:rgb(0,32,96);font-family:"Times New Roman","serif";font-size:10pt"></span> </div><div style="margin:0cm 0cm 0pt;text-align:left;line-height:normal;direction:ltr">
<div style="direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Ayman A. El-Badry, M.D.</span></div><div style="direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Prof of Medical Parasitology</span></div>
<p style="margin:0px!important;padding:0px!important;direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Head of Diagnostic & research Unit of Parasitic Diseases (DRUP)</span></p>
<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="margin:0px!important;padding:0px!important;direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Medical Parasitology Dept., </span><span style="color:rgb(23,54,93);font-family:'Times New Roman',serif;font-size:10pt">kasr Al-Ainy School of Medicine, </span><span style="color:rgb(23,54,93);font-family:'Times New Roman',serif;font-size:10pt">Cairo University</span></p>
<p style="margin:0px!important;padding:0px!important;direction:ltr"><span style="font-size:10pt;font-family:'Times New Roman';color:rgb(23,54,93);text-align:-webkit-auto">Postal Code 11559. </span><span style="font-family:'Times New Roman';color:rgb(23,54,93);font-size:10pt;text-align:-webkit-auto">POB: 109 El-Malek El Saleh Post Office - El Manial - Cairo - Egypt.</span></p>
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<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="margin:0px!important;padding:0px!important;direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">E-mail: <a href="mailto:aelbadry@kasralainy.edu.eg" target="_blank"><font color="#3b5998"></font></a><font color="#3b5998"><a href="mailto:aelbadry@kasralainy.edu.eg,aymanbb@hotmail.com,http://facebook.com/aymanbb" target="_blank">aelbadry@kasralainy.edu.eg</a></font><a href="mailto:aelbadry@kasralainy.edu.eg,aymanbb@hotmail.com,http://facebook.com/aymanbb" target="_blank"></a>,<a href="mailto:aymanbb@hotmail.com" target="_blank"><span style="color:rgb(0,102,204)">aymanbb@hotmail.com</span></a></span></p>
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