<div dir="ltr"><div class="gmail_extra">Dear All<div>kindly we would like to collect blood for molecular detection of visceral leishmania from village 500 Km away from the lab</div><div>is there a method to keep the sample intact tell reaching the lab on same day or may we have 24-48 hours delay</div>
<div>is there any one have experience and SOP with percoll, ficoll or histopque</div>could a buffy coat separated at field stand a time (24-48 hours) at room temperature without affecting Leishmania DNA or RNA extraction</div>
<div class="gmail_extra"><br></div><div class="gmail_extra">best regards </div><div class="gmail_extra"><br><div dir="ltr"><font size="3" face="Times New Roman">
</font><p style="margin:0cm 0cm 0pt;text-align:left;line-height:normal;direction:ltr"><strong><span style="color:rgb(79,129,189);font-size:10pt"><font face="Calibri">Ayman</font></span></strong></p><font size="3" face="Times New Roman">
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<div style="direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Ayman A. El-Badry, M.D.</span></div><div style="direction:ltr"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Prof of Medical Parasitology</span></div>
<p style="direction:ltr;margin:0px!important;padding:0px!important"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Head of Diagnostic & research Unit of Parasitic Diseases (DRUP)</span></p>
<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="direction:ltr;margin:0px!important;padding:0px!important"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">Medical Parasitology Dept., </span><span style="color:rgb(23,54,93);font-family:'Times New Roman',serif;font-size:10pt">kasr Al-Ainy School of Medicine, </span><span style="color:rgb(23,54,93);font-family:'Times New Roman',serif;font-size:10pt">Cairo University</span></p>
<p style="direction:ltr;margin:0px!important;padding:0px!important"><span style="font-size:10pt;font-family:'Times New Roman';color:rgb(23,54,93);text-align:-webkit-auto">Postal Code 11559. </span><span style="font-family:'Times New Roman';color:rgb(23,54,93);font-size:10pt;text-align:-webkit-auto">POB: 109 El-Malek El Saleh Post Office - El Manial - Cairo - Egypt.</span></p>
<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="text-align:-webkit-auto;margin:0px!important;padding:0px!important"><font face="Times New Roman"><span style="color:rgb(23,54,93);font-size:10pt">Tel.: </span></font><span style="color:rgb(23,54,93);font-family:'Times New Roman';font-size:14px">(+202) 23632733.</span></p>
<p style="text-align:-webkit-auto;margin:0px!important;padding:0px!important"><font face="Times New Roman"><span style="color:rgb(23,54,93);font-size:10pt">Fax: (+202) 23644383.</span><span style="color:rgb(79,129,189);font-size:10pt"></span></font></p>
<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="text-align:-webkit-auto;margin:0px!important;padding:0px!important"><font face="Times New Roman"><span style="color:rgb(23,54,93);font-size:10pt">Mobile phone: (+2)</span></font></p>
<font size="3" face="Times New Roman" style="text-align:-webkit-auto"></font><p style="direction:ltr;margin:0px!important;padding:0px!important"><span style="color:rgb(0,32,96);font-family:'Times New Roman',serif;font-size:10pt">E-mail: <a href="mailto:aelbadry@kasralainy.edu.eg" target="_blank"><font color="#3b5998"></font></a><font color="#3b5998"><a href="mailto:aelbadry@kasralainy.edu.eg,aymanbb@hotmail.com,http://facebook.com/aymanbb" target="_blank">aelbadry@kasralainy.edu.eg</a></font><a href="mailto:aelbadry@kasralainy.edu.eg,aymanbb@hotmail.com,http://facebook.com/aymanbb" target="_blank"></a>,<a href="mailto:aymanbb@hotmail.com" target="_blank"><span style="color:rgb(0,102,204)">aymanbb@hotmail.com</span></a></span></p>
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