[Leish-l] L.donovani in dogs : PCR discrimination

Kwang-Poo Chang kwangpoo.chang at rosalindfranklin.edu
Sat Jun 22 19:33:44 BRT 2013


Whatever the ways used to differntiate Ld vs Li may well be very valid
within the number of samples or isolates used for the given study.

I continue to believe that it is necessay to examine amastigotes
genomically in the clinical samples from all endemic sites.

KP

On Fri, Jun 21, 2013 at 12:41 AM, Mallorie Hide <mallorie.hide at ird.fr>wrote:

> Dear all,
>
> These cpb sequences can be used for Ldi/ldd discrimination by PCR (50pb
> needed).
>
> Best wishes,
>
> Mallorie
> *********************
> Dr Mallorie HIDE, Ph.D
>
>
>
> MIVEGEC (CNRS 5290-IRD 224-UM1-UM2) (ex-GEMI UR165/UMR2724)
> Equipe Genesys  (Génétique  et Biodiversité des Systèmes Infectieux)
>
> 911 av Agropolis BP 64501, 34394 MONTPELLIER Cedex 5, FRANCE
>
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> Fax : (33)4.67.41.62.99
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> *********************
>
>
> -----Message d'origine-----
> De : leish-l-bounces at lineu.icb.usp.br [mailto:
> leish-l-bounces at lineu.icb.usp.br] De la part de Patrick Bastien
> Envoyé : jeudi 20 juin 2013 09:19
> À : leish-l at lineu.icb.usp.br
> Objet : Re: [Leish-l] L.donovani in dogs?
>
> Yes. I believe that there are several. But at least several
> identifications from Sudan in Montpellier: see Dereure et al. Microbes
> Infect. 2003 Oct;5(12):1103-8.
> Please note that molecular techniques are not reliable to distinguish
> between infantum and donovani, which makes dubious the data of this type
> drawn from molecular ID. The ones quoted above were dont by isoenzymes.
> Best wishes
> P. Bastien
>
> Pr Patrick Bastien
> Director, Departement de Parasitologie-Mycologie Centre National de
> Reference des Leishmanioses Vice-Director, "MiVEGEC" unit research UMR5290
> CNRS/224 IRD/UM1/UM2 Faculte de Medecine, Université Montpellier 1
> 39 Av. Charles Flahault
> 34295 Montpellier cedex 5, France
>
>
> ----- Message de jayusp at hotmail.com ---------
>      Date : Thu, 20 Jun 2013 01:16:47 +0000
>       De : jeffrey shaw <jayusp at hotmail.com> Répondre à : jeffrey shaw <
> jayusp at hotmail.com>
>   Objet : [Leish-l] L.donovani in dogs?
>        À : Leish-L <leish-l at lineu.icb.usp.br>
>
>
> > Steve Reed  says:
> > "I am in Bangladesh...they think they have L donovani in dogs here Is
> > that the case in China? Or elsewhere?"
> > Does anybody know of a bone fide ID of donovani in dogs?
> > Jeff
> >
> >
> > --
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> >
>
>
> ----- Fin du message de jayusp at hotmail.com -----
>
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