Whatever the ways used to differntiate Ld vs Li may well be very valid within the number of samples or isolates used for the given study. <div><br></div><div>I continue to believe that it is necessay to examine amastigotes genomically in the clinical samples from all endemic sites.</div>
<div><br></div><div>KP<br><br><div class="gmail_quote">On Fri, Jun 21, 2013 at 12:41 AM, Mallorie Hide <span dir="ltr"><<a href="mailto:mallorie.hide@ird.fr" target="_blank">mallorie.hide@ird.fr</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Dear all,<br>
<br>
These cpb sequences can be used for Ldi/ldd discrimination by PCR (50pb needed).<br>
<br>
Best wishes,<br>
<br>
Mallorie<br>
*********************<br>
Dr Mallorie HIDE, Ph.D<br>
<br>
<br>
<br>
MIVEGEC (CNRS 5290-IRD 224-UM1-UM2) (ex-GEMI UR165/UMR2724)<br>
Equipe Genesys (Génétique et Biodiversité des Systèmes Infectieux)<br>
<br>
911 av Agropolis BP 64501, 34394 MONTPELLIER Cedex 5, FRANCE<br>
<br>
Tel : (33)4.67.41.63.91<br>
Fax : (33)4.67.41.62.99<br>
<br>
<a href="http://www.mivegec.ird.fr/" target="_blank">http://www.mivegec.ird.fr/</a><br>
*********************<br>
<br>
<br>
-----Message d'origine-----<br>
De : <a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a> [mailto:<a href="mailto:leish-l-bounces@lineu.icb.usp.br">leish-l-bounces@lineu.icb.usp.br</a>] De la part de Patrick Bastien<br>
Envoyé : jeudi 20 juin 2013 09:19<br>
À : <a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a><br>
Objet : Re: [Leish-l] L.donovani in dogs?<br>
<br>
Yes. I believe that there are several. But at least several identifications from Sudan in Montpellier: see Dereure et al. Microbes Infect. 2003 Oct;5(12):1103-8.<br>
Please note that molecular techniques are not reliable to distinguish between infantum and donovani, which makes dubious the data of this type drawn from molecular ID. The ones quoted above were dont by isoenzymes.<br>
Best wishes<br>
P. Bastien<br>
<br>
Pr Patrick Bastien<br>
Director, Departement de Parasitologie-Mycologie Centre National de Reference des Leishmanioses Vice-Director, "MiVEGEC" unit research UMR5290 CNRS/224 IRD/UM1/UM2 Faculte de Medecine, Université Montpellier 1<br>
39 Av. Charles Flahault<br>
34295 Montpellier cedex 5, France<br>
<br>
<br>
----- Message de <a href="mailto:jayusp@hotmail.com">jayusp@hotmail.com</a> ---------<br>
Date : Thu, 20 Jun 2013 01:16:47 +0000<br>
De : jeffrey shaw <<a href="mailto:jayusp@hotmail.com">jayusp@hotmail.com</a>> Répondre à : jeffrey shaw <<a href="mailto:jayusp@hotmail.com">jayusp@hotmail.com</a>><br>
Objet : [Leish-l] L.donovani in dogs?<br>
À : Leish-L <<a href="mailto:leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a>><br>
<br>
<br>
> Steve Reed says:<br>
> "I am in Bangladesh...they think they have L donovani in dogs here Is<br>
> that the case in China? Or elsewhere?"<br>
> Does anybody know of a bone fide ID of donovani in dogs?<br>
> Jeff<br>
><br>
><br>
> --<br>
> This email was sent by <a href="http://icb.usp.br" target="_blank">icb.usp.br</a><br>
><br>
<br>
<br>
----- Fin du message de <a href="mailto:jayusp@hotmail.com">jayusp@hotmail.com</a> -----<br>
<br>
<br>
<br>
_______________________________________________<br>
Leish-l mailing list<br>
<a href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br>
<a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" target="_blank">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>
<br>
--<br>
<br>
This email was sent by <a href="http://icb.usp.br" target="_blank">icb.usp.br</a><br>
<br>
<br>
--<br>
<br>
This email was sent by <a href="http://icb.usp.br" target="_blank">icb.usp.br</a><br>
<br>_______________________________________________<br>
Leish-l mailing list<br>
<a href="mailto:Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a><br>
<a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" target="_blank">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>
<br>
--<br>
This email was sent by <a href="http://icb.usp.br" target="_blank">icb.usp.br</a><br>
<br></blockquote></div><br></div>