[Leish-l] Fwd: hot spots

Tue Apr 9 03:03:15 BRT 2013

Dear All, I feel this is the right time to define parameters to be used to characterize axenic amastigote/ amastigote like/stressed out promastigotes before start using them,  as every one of us is worrying about the characterization and many of us have an experience of working with these  axenic amastigotes at various levels for almost more than one or two decades
Neena
--- On Fri, 5/4/13, McMahon-Pratt, Diane <diane.mcmahon-pratt at yale.edu> wrote:

From: McMahon-Pratt, Diane <diane.mcmahon-pratt at yale.edu>
Subject: Re: [Leish-l] Fwd:  hot spots
To: "Descoteaux, Albert" <Albert.Descoteaux at iaf.inrs.ca>, "Sunil Arora" <skarora_in at yahoo.com>
Cc: "Leish-L email post" <Leish-l at lineu.icb.usp.br>
Date: Friday, 5 April, 2013, 7:38 AM

#yiv1413518990 P {margin-top:0;margin-bottom:0;}

The real problem here is that people fail to characterize their organisms (axenic amastigotes). Some indeed are shocked promastigotes - others grow in culture and are amastigote-like
 (close approximations and in many characteristics identical).  Deflagellation does not mean amastigote. I have long felt people are sloppy in their characterization and hence we have a field where the "axenic amastigotes" connotation has variable meanings.
 I think this should be changed. I would not eschew the use of axenic amastigotes for the failure of some. We all need to carefully characterize there and use them accordingly.

From: leish-l-bounces at lineu.icb.usp.br [leish-l-bounces at lineu.icb.usp.br] on behalf of Descoteaux, Albert [Albert.Descoteaux at iaf.inrs.ca]

Sent: Friday, March 29, 2013 8:34 PM

To: Sunil Arora

Cc: Leish-L email post

Subject: Re: [Leish-l] Fwd: hot spots

Related to this issue, what are really the mammalian signals that induce promastigote-to-amastigote differentiation? 

Albert

Envoyé de mon iPad

Le 2013-03-29 à 15:58, "Sunil Arora" <skarora_in at yahoo.com> a écrit :

I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes
 in the infected host

best

sunil

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From: Clos Joachim <clos at bni-hamburg.de>

To: Leish-L email post <Leish-l at lineu.icb.usp.br>

Sent: Monday, 11 March 2013 8:16 PM

Subject: [Leish-l] Fwd: hot spots

Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out
 promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and
 interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
Cheers!

Am 07.02.2013 um 20:38 schrieb Carlos Costa:

What are the most polemic issues in leishmaniasis?

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