<table cellspacing="0" cellpadding="0" border="0" ><tr><td valign="top" style="font: inherit;">Dear All, <div>I feel this is the right time to define parameters to be used to characterize axenic amastigote/ amastigote like/stressed out promastigotes before start using them, as every one of us is worrying about <u>the characterization</u> and many of us have an experience of working with these axenic amastigotes at various levels for almost more than one or two decades<br>Neena<br>--- On <b>Fri, 5/4/13, McMahon-Pratt, Diane <i><diane.mcmahon-pratt@yale.edu></i></b> wrote:<br><blockquote style="border-left: 2px solid rgb(16, 16, 255); margin-left: 5px; padding-left: 5px;"><br>From: McMahon-Pratt, Diane <diane.mcmahon-pratt@yale.edu><br>Subject: Re: [Leish-l] Fwd: hot spots<br>To: "Descoteaux, Albert" <Albert.Descoteaux@iaf.inrs.ca>, "Sunil Arora" <skarora_in@yahoo.com><br>Cc: "Leish-L email post"
<Leish-l@lineu.icb.usp.br><br>Date: Friday, 5 April, 2013, 7:38 AM<br><br><div id="yiv1413518990">
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<div style="direction:ltr;font-family:Arial;color:#000000;font-size:12pt;">The real problem here is that people fail to characterize their organisms (axenic amastigotes). Some indeed are shocked promastigotes - others grow in culture and are amastigote-like
(close approximations and in many characteristics identical). Deflagellation does not mean amastigote. I have long felt people are sloppy in their characterization and hence we have a field where the "axenic amastigotes" connotation has variable meanings.
I think this should be changed. I would not eschew the use of axenic amastigotes for the failure of some. We all need to carefully characterize there and use them accordingly.
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<div style="direction:ltr;" id="yiv1413518990divRpF189581"><font color="#000000" face="Tahoma" size="2"><b>From:</b> leish-l-bounces@lineu.icb.usp.br [leish-l-bounces@lineu.icb.usp.br] on behalf of Descoteaux, Albert [Albert.Descoteaux@iaf.inrs.ca]<br>
<b>Sent:</b> Friday, March 29, 2013 8:34 PM<br>
<b>To:</b> Sunil Arora<br>
<b>Cc:</b> Leish-L email post<br>
<b>Subject:</b> Re: [Leish-l] Fwd: hot spots<br>
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<div>Related to this issue, what are really the mammalian signals that induce promastigote-to-amastigote differentiation? </div>
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<div>Albert<br>
<br>
Envoyé de mon iPad</div>
<div><br>
Le 2013-03-29 à 15:58, "Sunil Arora" <<a rel="nofollow" ymailto="mailto:skarora_in@yahoo.com" target="_blank" href="/mc/compose?to=skarora_in@yahoo.com">skarora_in@yahoo.com</a>> a écrit :<br>
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<div><span>I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes
in the infected host</span></div>
<div style="color:rgb(0,0,0);font-size:13px;font-family:verdana, helvetica, sans-serif;background-color:transparent;font-style:normal;">
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<div style="color:rgb(0,0,0);font-size:13px;font-family:verdana, helvetica, sans-serif;background-color:transparent;font-style:normal;">
<span>best</span></div>
<div style="color:rgb(0,0,0);font-size:13px;font-family:verdana, helvetica, sans-serif;background-color:transparent;font-style:normal;">
<span>sunil</span></div>
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<div><i><font color="#0000bf">---------------------------------------------------------------</font></i></div>
<div><i><font color="#0000bf">Dr Sunil K.Arora<br>
Professor <br>
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &<br>
NACO State Reference Laboratory,</font></i></div>
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<div><i><font color="#0000bf">Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)</font></i></div>
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<i><font color="#0000bf"><var id="yiv1413518990yui-ie-cursor"></var>Department of Immunopathology<br>
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</font><font color="#0000bf">email: <a rel="nofollow" ymailto="mailto:skarora_in@yahoo.com" target="_blank" href="/mc/compose?to=skarora_in@yahoo.com">
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<b><span style="font-weight:bold;">From:</span></b> Clos Joachim <<a rel="nofollow" ymailto="mailto:clos@bni-hamburg.de" target="_blank" href="/mc/compose?to=clos@bni-hamburg.de">clos@bni-hamburg.de</a>><br>
<b><span style="font-weight:bold;">To:</span></b> Leish-L email post <<a rel="nofollow" ymailto="mailto:Leish-l@lineu.icb.usp.br" target="_blank" href="/mc/compose?to=Leish-l@lineu.icb.usp.br">Leish-l@lineu.icb.usp.br</a>>
<br>
<b><span style="font-weight:bold;">Sent:</span></b> Monday, 11 March 2013 8:16 PM<br>
<b><span style="font-weight:bold;">Subject:</span></b> [Leish-l] Fwd: hot spots<br>
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<blockquote type="cite">
<div style="word-wrap:break-word;"><font class="yiv1413518990Apple-style-span" style="font-size:13px;" color="#010101" face="Baghdad">Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out
promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and
interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.</font>
<div><font class="yiv1413518990Apple-style-span" style="font-size:13px;" color="#010101" face="Baghdad">Cheers!</font></div>
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<div>Am 07.02.2013 um 20:38 schrieb Carlos Costa:</div>
<br class="yiv1413518990Apple-interchange-newline">
<blockquote type="cite">
<div dir="ltr"><font color="#000000"><font><font face="verdana,sans-serif">What are the most polemic issues in leishmaniasis?<br clear="all">
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