[Leish-l] Axenic amastigotes

[Kwang-Poo Chang]

Dear Robert,

I agree with everything you said. *Ultimately, human infection will be the
bottom line.*
*
*
*KP*


On Fri, Mar 29, 2013 at 3:42 PM, Duncan, Robert
<Robert.Duncan at fda.hhs.gov>wrote:

>  I have worked in the lab with axenic L. donovani for 20 years.
> Evaluating gene expression, cell biology and differentiation. They are an
> invaluable tool especially for molecular biology which requires a
> sufficient quantity of material to analyze.  However, I consider findings
> tentative until confirmed in cultured macrophage infections and rodent
> infections. We have recently begun studies of "full natural cycle
> transmissions": infected sand flies biting hamsters that develop visceral
> leishmaniasis, parasites from the infected hamsters fed to sand flies that
> are used to infect hamsters. Preliminary results suggest that cultured
> promastigotes are not a perfect model either. We certainly would not give
> up the years of discovery based on laboratory studies of cultured
> promastigotes.
>
> -Robert Duncan
>
>
>
> --
> * This email was sent by icb.usp.br *   ­­
>
> _______________________________________________
> Leish-l mailing list
> Leish-l at lineu.icb.usp.br
> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>
> --
> This email was sent by icb.usp.br
>
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lineu.icb.usp.br/pipermail/leish-l/attachments/20130404/2f69f100/attachment.htm>