[Leish-l] L. infantum in the hindgut?

[Carlos Costa]

Dear friends,

Thanks for all the hints for the primers to discriminate Leishmania from
Viannia. We will try some of them and check if the promastigotes Dr.
Mendonça and our group have found in the hindgut of dog fed Lu. longipalpis
correspond to L. infantum.

Best wishes,

Carlos.

2012/6/9 Chang, Kwang-Poo <KwangPoo.Chang at rosalindfranklin.edu>

> ** ** ** **
>
> Nagt sequence analysis can easily differentiate Viannia from Leishmania.
>  You will find this in our publication in 2007 that has been verified by
> additional data generated by **Carlos Muskus**, ****Medellin****.****
>
> ** **
>
> Genes encoding protein elements for the RNAi machinery are expected to
> exit only in the Viannia group, but I have not looked into this by
> designing primers for the ease of their PCR amplification versus nagt.
> These genes are expected to be absent in the Leishmania subgenus, producing
> no PCR products.  Such negative findings however may not be as good as
> something positive in both groups, but different between them. ****
>
> ** **
>
> KP****
>
> ** **
>  ------------------------------
>
> *From:* leish-l-bounces at lineu.icb.usp.br [mailto:
> leish-l-bounces at lineu.icb.usp.br] *On Behalf Of *Carlos Costa
> *Sent:* Saturday, June 02, 2012 5:28 PM
> *To:* Leish-L; Regiane Araujo; IVETE LOPES DE MENDONCA
>
> *Subject:* [Leish-l] L. infantum in the hindgut?
> ****
>
>  ** **
>
> Dear all,****
>
> ** **
>
> Dr. Ivete Mendonça, in ****Teresina**, **Brazil****, has consistently and
> systematically been observing promastigotes xenodiagnosis performed in dogs
> in the hindgut and rectal ampulla of Lutzomyia longipalpis. We are not sure
> if they are really L. infantum or Viannia. Does anybody has observed the
> same thing?****
>
> ** **
>
> By the way, any suggestions of primers to discriminate Leishmania from
> Viannia?****
>
> ** **
>
> Many thanks,****
>
> ** **
>
> Carlos.
> ****
>
> ** **
>
> ** **
>



-- 
Carlos H. N. Costa, MD, DSc.
Sociedade Brasileira de Medicina Tropical
(Brazilian Society of Tropical Medicine)
President

Universidade Federal do Piauí
Instituto de Doenças Tropicais Natan Portella
Rua Artur de Vasconcelos 151-Sul
64001-450 Teresina-PI
Brazil
Telephones: +55 86 3222-4377 (W),
+55 86 3221-3062 (W),
+55 86 3237-1075 (R).


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