[Leish-l] inquiry

Tue May 31 16:30:07 BRT 2011

Dear Colleagues,

We have very interesting experience with Sudanese L.donovani. This group of
parasite although are genetically homogenous they still cause diverse
clinical forms in Sudan ranging from VL, PKDL, ML to CL. We have isolated
L. donovani MON82 from classic cutaneous ulcers and typed them using both
isoenzyme and molecular typing techniques. Please check our manuscript
(Elamin et al. 2008. Trans R Soc Trop Med Hyg. 2008 Jan;102(1):54-7.). We
have analyzed several virulence genes comparing the isolates from the
diverse clinical forms and they turned to be are highly homologous.
Immunologically they induce different immune responses (TH1/TH2). We know
the vector of L.donovani VL isolates which is P. orientalis, but we still
don’t know the vector of cutaneous L. donovani parasite. We believe the
vector plays an important role in the pathogenesis and the outcome of L.
donovani infection together with the host immune response and may be some of
the parasite virulence factors. We will soon published our data on parasite
typing and host immune responses of the diverse clinical forms. 

Best regards

Maowia

Professor Maowia M. Mukhtar

Institute of Endemic Diseases

University of Khartoum

P.O. Box 11463, khartoum, Sudan

Mobile; +249912234268

Fax: +249183779712

From: leish-l-bounces at lineu.icb.usp.br
[mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Sharman
Sent: 31 May 2011 18:13
To: Petr Volf; Chang, Kwang-Poo; Hiro Goto; elfadil abass;
vishwamohan_katoch at yahoo.co.in; lalit Kant ICMR
Cc: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] inquiry

Hi Petr,

Thanks for enclosing the pdf. In fact the strain which we are dealing with
is not very difficult to grow but difficult to maintain, It is rK 39
positive and as I mentioned in the conversation below that even the serous
exudate from the ulcer is also positive for rK 39 dipstick. In between
because of my relocation to a different institute I was little away from
this work but I intend to resume this work in couple of months. Although in
past we tried to isolate the parasite from sand flies but did not succeed.
We will surely attempt again with the techniques described by you and
others. Contamination is the problem.

This particular focus seems to be a complex one.

NL Sharma 

From: Petr Volf <mailto:volf at cesnet.cz>  

Sent: Tuesday, May 31, 2011 12:36 PM

To: Chang, Kwang-Poo <mailto:KwangPoo.Chang at rosalindfranklin.edu>  ; Sharman
<mailto:nandlals at hotmail.com>  ; Hiro Goto <mailto:hgoto at usp.br>  ; elfadil
abass <mailto:elfadil_abass at yahoo.com>  

Cc: leish-l at lineu.icb.usp.br 

Subject: Re: [Leish-l] inquiry

Hi K.P.,

in Cukurova region, Turkey, cutaneous L. infantum (now it seems that we are
dealing with L.donovani/L. infantum hybrid) grew very poorely if we isolated
them from patients. Only 1 of 25 isolations was succesfull. However, the
same strain (confirmed by molecular methods) grew repeatedly and very well
if we isolated them from sandflies. It might be useful for Dr. Sharma try to
get isolates from sand flies. It is very laborious but very useful, in
Cukurova we got about dozen of isolates by this method (all identical).

Patients are rK39 negative, see attached paper.

Best wishes

Petr

----- Original Message ----- 

From: Chang, Kwang-Poo <mailto:KwangPoo.Chang at rosalindfranklin.edu>  

To: Sharman <mailto:nandlals at hotmail.com>  ; Hiro Goto <mailto:hgoto at usp.br>
; elfadil abass <mailto:elfadil_abass at yahoo.com>  

Cc: leish-l at lineu.icb.usp.br 

Sent: Sunday, May 22, 2011 1:39 AM

Subject: Re: [Leish-l] inquiry

Dr. NL Sharma has been working on an important CL endemic area along the
Satluj River valley to the south of Himalaya in India (Please correct me
should I be wrong for anything I said here and below). I had the good
fortune of visiting the site several years back courtesy of Dr. Sharma. 

I believe Dr. Sharma's finding is important, since the parasites there are
different from the familiar Indian L donovani in Bihar. The parasites are
refratory to in vitro cultivation. They do differentiate into promastigotes
and may grow a little, but  can't really be subcultured to establish
passageable lines. This is very much reminescent of L infantum in the
Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I
remember correctly. If so,  Satluj river valley is endemic to the infantile
CL.

Analyses of several batches of basically clinical CL samples from Dr. Sharma
showed evidence of L infantum, but also L tropica as well as a mixture of
the two in one sample. This is the same picture we have noted for samples
from Hatay, Turkey.

These observations make me wonder a lot about our current knowledge on the
clinico-epidemiology based on data collected previously by analyses of
cultured promastigotes from one or few 'representative samples'. Nowaday,
technology makes it very doable to work with biological samples for Leish
DNAs directly from sand flies, patients and reservoir animals.

KP

  _____  

From: leish-l-bounces at lineu.icb.usp.br on behalf of Sharman
Sent: Fri 5/20/2011 11:31 PM
To: Hiro Goto; elfadil abass
Cc: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] inquiry

Dear all
I agree with Hiro gito, We are working on a focus where the CL is
predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE
RESULTS WITH SERA as well as serous exudate from the lesion. The results are
dependent upon species.
NL Sharma

--------------------------------------------------
From: "Hiro Goto" <hgoto at usp.br>
Sent: Wednesday, May 18, 2011 12:09 AM
To: "elfadil abass" <elfadil_abass at yahoo.com>
Cc: <leish-l at lineu.icb.usp.br>
Subject: Re: [Leish-l] inquiry

> Dear all,
> In our oppinion, DAT and rK39 for those samples are not indicated  since
> these tests are produced for the diagnosis of visceral  leishmaniasis.
> In case of tegumentary leishmaniasis, it is very appropriate the
> observation of J.J. Shaw appointing species specificity of antibody
> response in these cases. Titers of antibodies are in general low in  these
> cases therefore depending on the species, it may result  negative. We have
> published a review recently in Expert Rev. Anti  Infect. Ther. 8(4),
> 419?433 (2010), Current diagnosis and treatment of  cutaneous and
> mucocutaneous leishmaniasis, where we raise this point.
> Hiro Goto
>
> Citando elfadil abass <elfadil_abass at yahoo.com>:
>
>> Dear all I would recommend using DAT and rK39 strip test to measure
>> antibody
>> responses and to evaluate the diagnostic efficiency for both tests  in
>> such group
>> of patients.
>>
>>  Elfadil Abass
>>
____________________________________________________________________________
____
>> Institute of Medical Microbiology and Hospital Hygiene
>> Philipps University Marburg
>> BMFZ / Hans-Meerwein Straße 2
>> D-35033 Marburg, Germany
>>
____________________________________________________________________________
____
>>
>>
>>
>>
>> ________________________________
>> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
>> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
>> <leish-l at lineu.icb.usp.br>
>> Sent: Fri, May 13, 2011 9:39:33 AM
>> Subject: Re: [Leish-l] inquiry
>>
>> Dear Saad first precaution to take is to separate your samples into
>> several
>> portions each (at least 5 each being no more than a few hundred(200-400)
>> microliters). This is a necessary precaution to avoid freezing and
>> thawing
>> several times the same sample which will be the case should you do
>> different
>> studies at different times which is unavoidable.
>>
>> What you can do is correlate the type(ulcerated, abscess, furuncle
>>  etc.. ) the
>> locale, the number and age of lesions with the levels of antibody in  the
>> sera of
>> these patgients and also monitor cell mediated immunity with leishmanin
>> skin
>> test!
>> I have just published (in print) an article about having circulating
>> parasites
>> in some of these patients with apparently pure cutaneous disease. It is
>> the
>> first paper that revealed this fact. It would be interesting to confirm
>> or
>> de-confirm this fact repeating what we did.
>> Best wishes
>>
>> Nuha Nuwayri-Salti MD
>>
>>
>>
>> AOA
>> Medical Honor Society
>>
>>
>>
>>
>> -----Original Message-----
>> From: leish-l-bounces at lineu.icb.usp.br
>> [mailto:leish-l-bounces at lineu.icb.usp.br]
>> On Behalf Of saad saad
>> Sent: Friday, April 29, 2011 11:23 PM
>> To: leish-l at lineu.icb.usp.br
>> Subject: [Leish-l] inquiry
>>
>> Dear all
>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
>> CL from south west of the country. I would like to have your
>> recommendations to start a good research line in CL using these sera.
>> Thanks in advance for your help
>> _______________________________________________
>> Leish-l mailing list
>> Leish-l at lineu.icb.usp.br
>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>> _______________________________________________
>> Leish-l mailing list
>> Leish-l at lineu.icb.usp.br
>> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>>
>
>
>
> Profa. Dra. Hiro Goto
> Laboratório de Soroepidemiologia e Imunobiologia
> Instituto de Medicina Tropical de São Paulo, USP
> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
> 05403-000 - São Paulo, SP
> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
> Fax. +55-11-3061-8270
>
> _______________________________________________
> Leish-l mailing list
> Leish-l at lineu.icb.usp.br
> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
>
_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l

  _____  

_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lineu.icb.usp.br/pipermail/leish-l/attachments/20110531/67242c5f/attachment-0001.htm>