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</o:shapelayout></xml><![endif]--></head><body bgcolor=white lang=EN-US link=blue vlink=purple id=MailContainerBody name="Compose message area"><div class=WordSection1><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Dear Colleagues,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>We have very interesting experience with Sudanese L.donovani. This group of parasite although are genetically homogenous they still cause diverse clinical forms in Sudan ranging from VL, PKDL, ML to CL. We have isolated L. donovani MON82 from classic cutaneous ulcers and typed them using both isoenzyme and molecular typing techniques. Please check our manuscript (Elamin et al. 2008. </span><span class=jrnl><span style='font-size:9.0pt;font-family:"Arial","sans-serif"'>Trans R Soc <span style='color:#1F497D'>Trop Med Hyg</span></span></span><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:#1F497D'>. 2008 Jan;102(1):54-7.). We have analyzed several virulence genes comparing the isolates from the diverse clinical forms and they turned to be are highly homologous. Immunologically they induce different immune responses (TH1/TH2). We know the vector of L.donovani VL isolates which is P. orientalis, but we still don’t know the vector of cutaneous L. donovani parasite. We believe the vector plays an important role in the pathogenesis and the outcome of L. donovani infection together with the host immune response and may be some of the parasite virulence factors. We will soon published our data on parasite typing and host immune responses of the diverse clinical forms. <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:#1F497D'>Best regards<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Arial","sans-serif";color:#1F497D'>Maowia</span><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><div><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Professor Maowia M. Mukhtar<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Institute of Endemic Diseases<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>University of Khartoum<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>P.O. Box 11463, khartoum, Sudan<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Mobile; +249912234268<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Fax: +249183779712<o:p></o:p></span></b></p></div><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm'><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> leish-l-bounces@lineu.icb.usp.br [mailto:leish-l-bounces@lineu.icb.usp.br] <b>On Behalf Of </b>Sharman<br><b>Sent:</b> 31 May 2011 18:13<br><b>To:</b> Petr Volf; Chang, Kwang-Poo; Hiro Goto; elfadil abass; vishwamohan_katoch@yahoo.co.in; lalit Kant ICMR<br><b>Cc:</b> leish-l@lineu.icb.usp.br<br><b>Subject:</b> Re: [Leish-l] inquiry<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'>Hi Petr,</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'>Thanks for enclosing the pdf. In fact the strain which we are dealing with is not very difficult to grow but difficult to maintain, It is rK 39 positive and as I mentioned in the conversation below that even the serous exudate from the ulcer is also positive for rK 39 dipstick. In between because of my relocation to a different institute I was little away from this work but I intend to resume this work in couple of months. Although in past we tried to isolate the parasite from sand flies but did not succeed. We will surely attempt again with the techniques described by you and others. Contamination is the problem.</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'>This particular focus seems to be a complex one.</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-family:"Calibri","sans-serif"'>NL Sharma</span> <o:p></o:p></p></div><div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p> </o:p></span></p></div><div><div><p class=MsoNormal style='background:whitesmoke'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz">Petr Volf</a> <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:whitesmoke'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Sent:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> Tuesday, May 31, 2011 12:36 PM<o:p></o:p></span></p></div><div><p class=MsoNormal style='background:whitesmoke'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>To:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> ; <a href="mailto:nandlals@hotmail.com" title="mailto:nandlals@hotmail.com CTRL + Click to follow link">Sharman</a> ; <a href="mailto:hgoto@usp.br" title="mailto:hgoto@usp.br CTRL + Click to follow link">Hiro Goto</a> ; <a href="mailto:elfadil_abass@yahoo.com" title="mailto:elfadil_abass@yahoo.com CTRL + Click to follow link">elfadil abass</a> <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:whitesmoke'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Cc:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a> <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:whitesmoke'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Subject:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> Re: [Leish-l] inquiry<o:p></o:p></span></p></div></div></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Hi K.P.,</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>in Cukurova region, Turkey, cutaneous L. infantum (now it seems that we are dealing with L.donovani/L. infantum hybrid) grew very poorely if we isolated them from patients. Only 1 of 25 isolations was succesfull. However, the same strain (confirmed by molecular methods) grew repeatedly and very well if we isolated them from sandflies. It might be useful for Dr. Sharma try to get isolates from sand flies. It is very laborious but very useful, in Cukurova we got about dozen of isolates by this method (all identical).</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Patients are rK39 negative, see attached paper.</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Best wishes</span><o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Petr</span><o:p></o:p></p></div><blockquote style='border:none;border-left:solid black 1.5pt;padding:0cm 0cm 0cm 4.0pt;margin-left:3.75pt;margin-top:5.0pt;margin-right:0cm;margin-bottom:5.0pt'><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>----- Original Message ----- <o:p></o:p></span></p></div><div><p class=MsoNormal style='background:#E4E4E4'><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>From:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu">Chang, Kwang-Poo</a> <o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>To:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com">Sharman</a> ; <a href="mailto:hgoto@usp.br" title="hgoto@usp.br">Hiro Goto</a> ; <a href="mailto:elfadil_abass@yahoo.com" title="elfadil_abass@yahoo.com">elfadil abass</a> <o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Cc:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> <a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br">leish-l@lineu.icb.usp.br</a> <o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Sent:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> Sunday, May 22, 2011 1:39 AM<o:p></o:p></span></p></div><div><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Subject:</span></b><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'> Re: [Leish-l] inquiry<o:p></o:p></span></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div id=idOWAReplyText41530><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";color:black'>Dr. NL Sharma has been working on an important CL endemic area along the Satluj River valley to the south of Himalaya in India (Please correct me should I be wrong for anything I said here and below). I had the good fortune of visiting the site several years back courtesy of Dr. Sharma. </span><o:p></o:p></p></div><div><p class=MsoNormal> <o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>I believe Dr. Sharma's finding is important, since the parasites there are different from the familiar Indian L donovani in Bihar. The parasites are refratory to in vitro cultivation. They do differentiate into promastigotes and may grow a little, but can't really be subcultured to establish passageable lines. This is very much reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I remember correctly. If so, Satluj river valley is endemic to the infantile CL.</span><o:p></o:p></p></div><div><p class=MsoNormal> <o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey.</span><o:p></o:p></p></div><div><p class=MsoNormal> <o:p></o:p></p></div><div><p class=MsoNormal><span style='font-family:"Arial","sans-serif"'>These observations make me wonder a lot about our current knowledge on the clinico-epidemiology based on data collected previously by analyses of cultured promastigotes from one or few 'representative samples'. Nowaday, technology makes it very doable to work with biological samples for Leish DNAs directly from sand flies, patients and reservoir animals.</span><o:p></o:p></p></div><div><p class=MsoNormal> <o:p></o:p></p></div><div><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>KP</span><o:p></o:p></p></div><div><p class=MsoNormal> <o:p></o:p></p></div></div><div id=idSignature79278><div><p class=MsoNormal> <o:p></o:p></p></div><div><div class=MsoNormal align=center style='text-align:center'><hr size=2 width="100%" align=center></div><p class=MsoNormal style='margin-bottom:12.0pt'><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> leish-l-bounces@lineu.icb.usp.br on behalf of Sharman<br><b>Sent:</b> Fri 5/20/2011 11:31 PM<br><b>To:</b> Hiro Goto; elfadil abass<br><b>Cc:</b> leish-l@lineu.icb.usp.br<br><b>Subject:</b> Re: [Leish-l] inquiry</span><o:p></o:p></p></div></div><div><p><span style='font-size:10.0pt'>Dear all<br>I agree with Hiro gito, We are working on a focus where the CL is<br>predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE<br>RESULTS WITH SERA as well as serous exudate from the lesion. The results are<br>dependent upon species.<br>NL Sharma<br><br>--------------------------------------------------<br>From: "Hiro Goto" <hgoto@usp.br><br>Sent: Wednesday, May 18, 2011 12:09 AM<br>To: "elfadil abass" <elfadil_abass@yahoo.com><br>Cc: <leish-l@lineu.icb.usp.br><br>Subject: Re: [Leish-l] inquiry<br><br>> Dear all,<br>> In our oppinion, DAT and rK39 for those samples are not indicated since<br>> these tests are produced for the diagnosis of visceral leishmaniasis.<br>> In case of tegumentary leishmaniasis, it is very appropriate the<br>> observation of J.J. Shaw appointing species specificity of antibody<br>> response in these cases. Titers of antibodies are in general low in these<br>> cases therefore depending on the species, it may result negative. We have<br>> published a review recently in Expert Rev. Anti Infect. Ther. 8(4),<br>> 419?433 (2010), Current diagnosis and treatment of cutaneous and<br>> mucocutaneous leishmaniasis, where we raise this point.<br>> Hiro Goto<br>><br>> Citando elfadil abass <elfadil_abass@yahoo.com>:<br>><br>>> Dear all I would recommend using DAT and rK39 strip test to measure<br>>> antibody<br>>> responses and to evaluate the diagnostic efficiency for both tests in<br>>> such group<br>>> of patients.<br>>><br>>> Elfadil Abass<br>>> ________________________________________________________________________________<br>>> Institute of Medical Microbiology and Hospital Hygiene<br>>> Philipps University Marburg<br>>> BMFZ / Hans-Meerwein Straße 2<br>>> D-35033 Marburg, Germany<br>>> ________________________________________________________________________________<br>>><br>>><br>>><br>>><br>>> ________________________________<br>>> From: Nuha Nuwayri-Salti <racha@aub.edu.lb><br>>> To: saad saad <saad1426@gmail.com>; "leish-l@lineu.icb.usp.br"<br>>> <leish-l@lineu.icb.usp.br><br>>> Sent: Fri, May 13, 2011 9:39:33 AM<br>>> Subject: Re: [Leish-l] inquiry<br>>><br>>> Dear Saad first precaution to take is to separate your samples into<br>>> several<br>>> portions each (at least 5 each being no more than a few hundred(200-400)<br>>> microliters). This is a necessary precaution to avoid freezing and<br>>> thawing<br>>> several times the same sample which will be the case should you do<br>>> different<br>>> studies at different times which is unavoidable.<br>>><br>>> What you can do is correlate the type(ulcerated, abscess, furuncle<br>>> etc.. ) the<br>>> locale, the number and age of lesions with the levels of antibody in the<br>>> sera of<br>>> these patgients and also monitor cell mediated immunity with leishmanin<br>>> skin<br>>> test!<br>>> I have just published (in print) an article about having circulating<br>>> parasites<br>>> in some of these patients with apparently pure cutaneous disease. It is<br>>> the<br>>> first paper that revealed this fact. It would be interesting to confirm<br>>> or<br>>> de-confirm this fact repeating what we did.<br>>> Best wishes<br>>><br>>> Nuha Nuwayri-Salti MD<br>>><br>>><br>>><br>>> AOA<br>>> Medical Honor Society<br>>><br>>><br>>><br>>><br>>> -----Original Message-----<br>>> From: leish-l-bounces@lineu.icb.usp.br<br>>> [<a href="mailto:leish-l-bounces@lineu.icb.usp.br">mailto:leish-l-bounces@lineu.icb.usp.br</a>]<br>>> On Behalf Of saad saad<br>>> Sent: Friday, April 29, 2011 11:23 PM<br>>> To: leish-l@lineu.icb.usp.br<br>>> Subject: [Leish-l] inquiry<br>>><br>>> Dear all<br>>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of<br>>> CL from south west of the country. I would like to have your<br>>> recommendations to start a good research line in CL using these sera.<br>>> Thanks in advance for your help<br>>> _______________________________________________<br>>> Leish-l mailing list<br>>> Leish-l@lineu.icb.usp.br<br>>> <a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>>> _______________________________________________<br>>> Leish-l mailing list<br>>> Leish-l@lineu.icb.usp.br<br>>> <a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>>><br>><br>><br>><br>> Profa. Dra. Hiro Goto<br>> Laboratório de Soroepidemiologia e Imunobiologia<br>> Instituto de Medicina Tropical de São Paulo, USP<br>> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar<br>> 05403-000 - São Paulo, SP<br>> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027<br>> Fax. +55-11-3061-8270<br>><br>> _______________________________________________<br>> Leish-l mailing list<br>> Leish-l@lineu.icb.usp.br<br>> <a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>><br>_______________________________________________<br>Leish-l mailing list<br>Leish-l@lineu.icb.usp.br<br><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a></span><o:p></o:p></p></div><div class=MsoNormal align=center style='text-align:center'><hr size=2 width="100%" align=center></div><p class=MsoNormal>_______________________________________________<br>Leish-l mailing list<br>Leish-l@lineu.icb.usp.br<br>http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l<o:p></o:p></p></blockquote></div></body></html>