[Leish-l] Leish-l Digest, Vol 41, Issue 9

Tue May 31 05:19:24 BRT 2011

Dear Dr Raj:

I think you mean PKDL (post kala azar dermal leishmanisis) caused by L.
donovani and it usually seen as a late complication of the disease. PKDL has
been reported in India, Kenya and Sudan.

Thank you 
R/
Hanafi

Hanafi  A.  Hanafi, Ph. D.
Medical Research Scientist
Vector Biology Research Program
U.S. Naval Medical Research Unit No.3
Cairo, Egypt
Tel & Fax:  ++2-02-2342-3090
E-mail: hanafi.hanafi.ctr.eg at med.navy.mil
        yahanafi at yahoo.com

-----Original Message-----
From: leish-l-bounces at lineu.icb.usp.br
[mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Raj
Sent: Monday, May 23, 2011 4:59 AM
To: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] Leish-l Digest, Vol 41, Issue 9

Hello Dr NL Sharma,
Could you please be explicit about cutanoeus lesions caused by L. donovani. 
I am interested to know the geographical area where L. donovani producing 
cutanoues manifestations.
Thanks

Dr Raj
Cellabs
----- Original Message ----- 
From: <leish-l-request at lineu.icb.usp.br>
To: <leish-l at lineu.icb.usp.br>
Sent: Monday, May 23, 2011 1:00 AM
Subject: Leish-l Digest, Vol 41, Issue 9

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>   1. Re: inquiry (Sharman)
>
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> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 21 May 2011 10:01:04 +0530
> From: "Sharman" <nandlals at hotmail.com>
> Subject: Re: [Leish-l] inquiry
> To: "Hiro Goto" <hgoto at usp.br>, "elfadil abass"
> <elfadil_abass at yahoo.com>
> Cc: leish-l at lineu.icb.usp.br
> Message-ID: <SNT105-DS945FBBAC8F50AEF312B11D8700 at phx.gbl>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=response
>
> Dear all
> I agree with Hiro gito, We are working on a focus where the CL is
> predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE
> RESULTS WITH SERA as well as serous exudate from the lesion. The results 
> are
> dependent upon species.
> NL Sharma
>
> --------------------------------------------------
> From: "Hiro Goto" <hgoto at usp.br>
> Sent: Wednesday, May 18, 2011 12:09 AM
> To: "elfadil abass" <elfadil_abass at yahoo.com>
> Cc: <leish-l at lineu.icb.usp.br>
> Subject: Re: [Leish-l] inquiry
>
>> Dear all,
>> In our oppinion, DAT and rK39 for those samples are not indicated  since
>> these tests are produced for the diagnosis of visceral  leishmaniasis.
>> In case of tegumentary leishmaniasis, it is very appropriate the
>> observation of J.J. Shaw appointing species specificity of antibody
>> response in these cases. Titers of antibodies are in general low in 
>> these
>> cases therefore depending on the species, it may result  negative. We 
>> have
>> published a review recently in Expert Rev. Anti  Infect. Ther. 8(4),
>> 419?433 (2010), Current diagnosis and treatment of  cutaneous and
>> mucocutaneous leishmaniasis, where we raise this point.
>> Hiro Goto
>>
>> Citando elfadil abass <elfadil_abass at yahoo.com>:
>>
>>> Dear all I would recommend using DAT and rK39 strip test to measure
>>> antibody
>>> responses and to evaluate the diagnostic efficiency for both tests  in
>>> such group
>>> of patients.
>>>
>>>  Elfadil Abass
>>>
____________________________________________________________________________
____
>>> Institute of Medical Microbiology and Hospital Hygiene
>>> Philipps University Marburg
>>> BMFZ / Hans-Meerwein Stra?e 2
>>> D-35033 Marburg, Germany
>>>
____________________________________________________________________________
____
>>>
>>>
>>>
>>>
>>> ________________________________
>>> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
>>> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
>>> <leish-l at lineu.icb.usp.br>
>>> Sent: Fri, May 13, 2011 9:39:33 AM
>>> Subject: Re: [Leish-l] inquiry
>>>
>>> Dear Saad first precaution to take is to separate your samples into
>>> several
>>> portions each (at least 5 each being no more than a few hundred(200-400)
>>> microliters). This is a necessary precaution to avoid freezing and
>>> thawing
>>> several times the same sample which will be the case should you do
>>> different
>>> studies at different times which is unavoidable.
>>>
>>> What you can do is correlate the type(ulcerated, abscess, furuncle
>>>  etc.. ) the
>>> locale, the number and age of lesions with the levels of antibody in 
>>> the
>>> sera of
>>> these patgients and also monitor cell mediated immunity with leishmanin
>>> skin
>>> test!
>>> I have just published (in print) an article about having circulating
>>> parasites
>>> in some of these patients with apparently pure cutaneous disease. It is
>>> the
>>> first paper that revealed this fact. It would be interesting to confirm
>>> or
>>> de-confirm this fact repeating what we did.
>>> Best wishes
>>>
>>> Nuha Nuwayri-Salti MD
>>>
>>>
>>>
>>> AOA
>>> Medical Honor Society
>>>
>>>
>>>
>>>
>>> -----Original Message-----
>>> From: leish-l-bounces at lineu.icb.usp.br
>>> [mailto:leish-l-bounces at lineu.icb.usp.br]
>>> On Behalf Of saad saad
>>> Sent: Friday, April 29, 2011 11:23 PM
>>> To: leish-l at lineu.icb.usp.br
>>> Subject: [Leish-l] inquiry
>>>
>>> Dear all
>>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
>>> CL from south west of the country. I would like to have your
>>> recommendations to start a good research line in CL using these sera.
>>> Thanks in advance for your help
>>> _______________________________________________
>>> Leish-l mailing list
>>> Leish-l at lineu.icb.usp.br
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>>
>>
>>
>> Profa. Dra. Hiro Goto
>> Laborat?rio de Soroepidemiologia e Imunobiologia
>> Instituto de Medicina Tropical de S?o Paulo, USP
>> Av. Dr. En?as de Carvalho Aguiar, 470, pr?dio II, quarto andar
>> 05403-000 - S?o Paulo, SP
>> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
>> Fax. +55-11-3061-8270
>>
>> _______________________________________________
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>
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