[Leish-l] inquiry

John David jdavid at hsph.harvard.edu
Mon Jun 6 01:18:08 BRT 2011


The Themomed instrument shown below can produce accurate 50 degrees C   
plus or minus 0.2 degrees temperature by radio wave.
from Themorsurgery Technologies. Picture below.
Two papers of a trial in Brazil and one in Afghanistan on CL below.




John David


On Jun 4, 2011, at 3:39 PM, Chang, Kwang-Poo wrote:

> I gather from John David and from previous reports about this in the  
> WLs, it is a special device to generate radio wave, producing heat  
> that is slightly above body temperature, but deep penetrating to  
> kill Leishmania in the lesion. Abhay may well be able to provide the  
> device for trials ? He can certainly provide you with a lot more  
> information.
>
> KP
>
> From: Sharmanl [mailto:nandlals at hotmail.com]
> Sent: Saturday, June 04, 2011 12:51 AM
> To: Satoskar, Abhay; Chang, Kwang-Poo; Raj; Petr Volf; leish-l at lineu.icb.usp.br 
> ; hgoto at usp.br; elfadil_abass at yahoo.com; vishwamohan_katoch at yahoo.co.in
> Subject: Re: [Leish-l] inquiry
>
> Dear Dr. Satoskar,
> Thanks for this piece of information and also the reprint. However,  
> I have some questions regarding this. What exactly the  
> Radiofrequency heat therapy means , Exactly what type of instrument  
> is this . We do have dermatological radiofrequency cautery machines,  
> is it the same; but here the electrodes are there which cut /  
> coagulate the tissue. I am not aware of any appliance in our set up  
> which creates field generating heat/ temperature in a granulamatous  
> tissue of the size say few cms.  I would surely like to know.
> Thanks once again
> NLS
>
> From: Satoskar, Abhay
> Sent: Saturday, June 04, 2011 4:19 AM
> To: 'Sharman' ; Chang, Kwang-Poo ; Raj ; Petr Volf ; leish-l at lineu.icb.usp.br 
>  ; hgoto at usp.br ; elfadil_abass at yahoo.com ; vishwamohan_katoch at yahoo.co.in
> Subject: RE: [Leish-l] inquiry
>
> Dear Dr. Sharma:
> I would be interested in know how you are treating your CL patients?  
> I presume intralesional SSG.
> Would you be interested in using radio-frequency induced topical  
> heat therapy which we have found to effective even in SSG non- 
> responsive cases of CL in India. I am enclosing a reprint of a paper  
> for your information. Let me know
> Thanks
> Abhay
>
> Abhay R. Satoskar M.D., Ph.D.
> Professor
> Departments of Pathology and Microbiology
> Starling Loving Hall M418
> The Ohio State University
> Columbus, Ohio
> Tel: 614-366-3417
>
>
>
> From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br 
> ] On Behalf Of Sharman
> Sent: Friday, June 03, 2011 12:15 PM
> To: Chang, Kwang-Poo; Raj; Petr Volf; leish-l at lineu.icb.usp.br; hgoto at usp.br 
> ; elfadil_abass at yahoo.com; vishwamohan_katoch at yahoo.co.in
> Subject: Re: [Leish-l] inquiry
>
> Dear KP,
> Yes you remember correctly that these strains are different from  
> common Indian Strain of L. donovani of Bihar. The three strains  
> examined at London School of tropical Medicine with each having  
> different homologies but they were also not L infantum. However,  
> some other strains examined at Rosalind franklin University had  
> results which you are mentioning. We had two dogs positive for rK 39  
> and one which could follow, died after about 5 months.
> Dr. Sunil Arora had also experience of handling these strains but  
> also could not maintain for long.
> NL Sharma
>
> From: Chang, Kwang-Poo
> Sent: Thursday, June 02, 2011 11:40 PM
> To: Sharman ; Raj ; Petr Volf ; leish-l at lineu.icb.usp.br ; hgoto at usp.br 
>  ; elfadil_abass at yahoo.com ; vishwamohan_katoch at yahoo.co.in
> Subject: RE: [Leish-l] inquiry
>
> Dear Dr. Sharma,
>
> Is the L donovani in the Satluj River Valley different from the L  
> donovani in Bihar ? This is an important point.
>
> The genetic site we examined did reveal identity of your samples  
> with L infantum, but differs from L donovani from Bihar and Sudan.  
> Have you not found rK39+ dogs ?  In one of your samples, we saw  
> evidence of both L infantum and L tropica. Whether this is a hybrid  
> or a mixture of the two cannot be certain.
>
> It would be good if Petr Volf could have an opportunity of looking  
> into the sand flies there ?
>
> KP
>
> From: Sharman [mailto:nandlals at hotmail.com]
> Sent: Wednesday, June 01, 2011 11:40 AM
> To: Raj; Petr Volf; Chang, Kwang-Poo; leish-l at lineu.icb.usp.br; hgoto at usp.br 
> ; elfadil_abass at yahoo.com; vishwamohan_katoch at yahoo.co.in
> Subject: Re: [Leish-l] inquiry
>
> Dear all,
> The discussion is getting very interesting. To let you know our  
> focus of CL in India, I.e. Satluj Valley focus has got L donovani  
> predominantly and L . tropica as well. Two Labs conformed L donovani  
> with approximately 4: 1 ratio  to L. tropica. Some more samples   
> tested by Dr. Chang revealed L infantum and L tropica. Results of  
> First study were reported in AJTMH in 2005. L.infantum results still  
> unpublished. Another peculiar observation we observed in a child  
> patient was that after injecting SSG intralesionally white spots  
> developed at the injection sites. Whether this was toxicity of SSG  
> or it is a counterpart of PKDL as L. donovani is the cause is a  
> question still to be answered. As I mentioned earlier some of the  
> patients' lesional exudate was also rK 39 positive. Well number of  
> questions unanswered. Will try to find answers.
> NLS
>
> From: Raj
> Sent: Wednesday, June 01, 2011 8:07 AM
> To: Petr Volf ; Chang, Kwang-Poo ; leish-l at lineu.icb.usp.br ; nandlals at hotmail.com 
>  ; hgoto at usp.br ; elfadil_abass at yahoo.com
> Subject: Re: [Leish-l] inquiry
>
>  G'Day Dr Petr Volf,
> Thank you very much for your paper. Excellent molecular typing of  
> parasites.
> As you see my correspondence below, we have a antibody ELISA where  
> we use exoantigens from individual L. infantum and L. tropica.
>  If you are interested I can test plasma/serum samples for presence  
> of antibody response. If so  please let me know.
>
> Raj
> Cellabs
>
>
> G'Day Dr Hanafi,
> My enquiry was to know whether L. donovani in India produces a  
> cutaneous form of lesion or not (I am not referring to PKDL form).  
> There are many reports from SriLanka about the cutaneous  
> manifestations by L. donovani. The PKDL although is a  cutaneous  
> response appears very late in the infection process and this is  
> ascribed to be a protective response against the parasite.
>
> Prof Chang mentioned
> "Analyses of several batches of basically clinical CL samples from  
> Dr. Sharma showed evidence of L infantum, but also L tropica as well  
> as a mixture of the two in one sample. This is the same picture we  
> have noted for samples from Hatay, Turkey." This is from Sutlej  
> region." It means this cutaneous  complex requires some investigation.
>
> I raise species-specific exo-antigens from L. tropica and L infantum  
> and I am prepared to test the serum /plasma samples for presence of  
> antibodies.  If Dr NL Sharma is prepared to send me specimens as  
> well as clinical plasma or serum samples I would be interested to  
> investigate the antibody response in these situations.
>
> Dr Raj
> Cellabs
> ----- Original Message -----
> From: Petr Volf
> To: Raj
> Sent: Tuesday, May 31, 2011 5:08 PM
> Subject: Fw: [Leish-l] inquiry
>
> Dear Dr Raj,
> I saw your question to Dr. Sharma. I am forwarding my response to  
> him as well the paper which might be interesting also for you.
> Best wishes
> Petr Volf
>
> ----- Original Message -----
> From: Petr Volf
> To: Chang, Kwang-Poo ; Sharman ; Hiro Goto ; elfadil abass
> Cc: leish-l at lineu.icb.usp.br
> Sent: Tuesday, May 31, 2011 9:06 AM
> Subject: Re: [Leish-l] inquiry
>
> Hi K.P.,
> in Cukurova region, Turkey, cutaneous L. infantum (now it seems that  
> we are dealing with L.donovani/L. infantum hybrid) grew very poorely  
> if we isolated them from patients. Only 1 of 25 isolations was  
> succesfull. However, the same strain (confirmed by molecular  
> methods) grew repeatedly and very well if we isolated them from  
> sandflies. It might be useful for Dr. Sharma try to get isolates  
> from sand flies. It is very laborious but very useful, in Cukurova  
> we got about dozen of isolates by this method (all identical).
> Patients are rK39 negative, see attached paper.
> Best wishes
> Petr
> ----- Original Message -----
> From: Chang, Kwang-Poo
> To: Sharman ; Hiro Goto ; elfadil abass
> Cc: leish-l at lineu.icb.usp.br
> Sent: Sunday, May 22, 2011 1:39 AM
> Subject: Re: [Leish-l] inquiry
>
> Dr. NL Sharma has been working on an important CL endemic area along  
> the Satluj River valley to the south of Himalaya in India (Please  
> correct me should I be wrong for anything I said here and below). I  
> had the good fortune of visiting the site several years back  
> courtesy of Dr. Sharma.
>
> I believe Dr. Sharma's finding is important, since the parasites  
> there are different from the familiar Indian L donovani in Bihar.  
> The parasites are refratory to in vitro cultivation. They do  
> differentiate into promastigotes and may grow a little, but  can't  
> really be subcultured to establish passageable lines. This is very  
> much reminescent of L infantum in the Mediterranean area. I recall  
> Dr. Sharma has also found rK39+ dogs, if I remember correctly. If  
> so,  Satluj river valley is endemic to the infantile CL.
>
> Analyses of several batches of basically clinical CL samples from  
> Dr. Sharma showed evidence of L infantum, but also L tropica as well  
> as a mixture of the two in one sample. This is the same picture we  
> have noted for samples from Hatay, Turkey.
>
> These observations make me wonder a lot about our current knowledge  
> on the clinico-epidemiology based on data collected previously by  
> analyses of cultured promastigotes from one or few 'representative  
> samples'. Nowaday, technology makes it very doable to work with  
> biological samples for Leish DNAs directly from sand flies, patients  
> and reservoir animals.
>
> KP
>
>
> From: leish-l-bounces at lineu.icb.usp.br on behalf of Sharman
> Sent: Fri 5/20/2011 11:31 PM
> To: Hiro Goto; elfadil abass
> Cc: leish-l at lineu.icb.usp.br
> Subject: Re: [Leish-l] inquiry
>
> Dear all
> I agree with Hiro gito, We are working on a focus where the CL is
> predominantly caused by L. donovani, and the rK 39 STRIPS GIVE  
> POSITIVE
> RESULTS WITH SERA as well as serous exudate from the lesion. The  
> results are
> dependent upon species.
> NL Sharma
>
> --------------------------------------------------
> From: "Hiro Goto" <hgoto at usp.br>
> Sent: Wednesday, May 18, 2011 12:09 AM
> To: "elfadil abass" <elfadil_abass at yahoo.com>
> Cc: <leish-l at lineu.icb.usp.br>
> Subject: Re: [Leish-l] inquiry
>
> > Dear all,
> > In our oppinion, DAT and rK39 for those samples are not indicated   
> since
> > these tests are produced for the diagnosis of visceral   
> leishmaniasis.
> > In case of tegumentary leishmaniasis, it is very appropriate the
> > observation of J.J. Shaw appointing species specificity of antibody
> > response in these cases. Titers of antibodies are in general low  
> in  these
> > cases therefore depending on the species, it may result  negative.  
> We have
> > published a review recently in Expert Rev. Anti  Infect. Ther. 8(4),
> > 419?433 (2010), Current diagnosis and treatment of  cutaneous and
> > mucocutaneous leishmaniasis, where we raise this point.
> > Hiro Goto
> >
> > Citando elfadil abass <elfadil_abass at yahoo.com>:
> >
> >> Dear all I would recommend using DAT and rK39 strip test to measure
> >> antibody
> >> responses and to evaluate the diagnostic efficiency for both  
> tests  in
> >> such group
> >> of patients.
> >>
> >>  Elfadil Abass
> >>  
> ________________________________________________________________________________
> >> Institute of Medical Microbiology and Hospital Hygiene
> >> Philipps University Marburg
> >> BMFZ / Hans-Meerwein Straße 2
> >> D-35033 Marburg, Germany
> >>  
> ________________________________________________________________________________
> >>
> >>
> >>
> >>
> >> ________________________________
> >> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
> >> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
> >> <leish-l at lineu.icb.usp.br>
> >> Sent: Fri, May 13, 2011 9:39:33 AM
> >> Subject: Re: [Leish-l] inquiry
> >>
> >> Dear Saad first precaution to take is to separate your samples into
> >> several
> >> portions each (at least 5 each being no more than a few  
> hundred(200-400)
> >> microliters). This is a necessary precaution to avoid freezing and
> >> thawing
> >> several times the same sample which will be the case should you do
> >> different
> >> studies at different times which is unavoidable.
> >>
> >> What you can do is correlate the type(ulcerated, abscess, furuncle
> >>  etc.. ) the
> >> locale, the number and age of lesions with the levels of antibody  
> in  the
> >> sera of
> >> these patgients and also monitor cell mediated immunity with  
> leishmanin
> >> skin
> >> test!
> >> I have just published (in print) an article about having  
> circulating
> >> parasites
> >> in some of these patients with apparently pure cutaneous disease.  
> It is
> >> the
> >> first paper that revealed this fact. It would be interesting to  
> confirm
> >> or
> >> de-confirm this fact repeating what we did.
> >> Best wishes
> >>
> >> Nuha Nuwayri-Salti MD
> >>
> >>
> >>
> >> AOA
> >> Medical Honor Society
> >>
> >>
> >>
> >>
> >> -----Original Message-----
> >> From: leish-l-bounces at lineu.icb.usp.br
> >> [mailto:leish-l-bounces at lineu.icb.usp.br]
> >> On Behalf Of saad saad
> >> Sent: Friday, April 29, 2011 11:23 PM
> >> To: leish-l at lineu.icb.usp.br
> >> Subject: [Leish-l] inquiry
> >>
> >> Dear all
> >> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive  
> case of
> >> CL from south west of the country. I would like to have your
> >> recommendations to start a good research line in CL using these  
> sera.
> >> Thanks in advance for your help
> >> _______________________________________________
> >> Leish-l mailing list
> >> Leish-l at lineu.icb.usp.br
> >> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
> >> _______________________________________________
> >> Leish-l mailing list
> >> Leish-l at lineu.icb.usp.br
> >> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
> >>
> >
> >
> >
> > Profa. Dra. Hiro Goto
> > Laboratório de Soroepidemiologia e Imunobiologia
> > Instituto de Medicina Tropical de São Paulo, USP
> > Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
> > 05403-000 - São Paulo, SP
> > Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
> > Fax. +55-11-3061-8270
> >
> > _______________________________________________
> > Leish-l mailing list
> > Leish-l at lineu.icb.usp.br
> > http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
> >
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