<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div></div><div><br><div><div>John David</div><div><br></div><div><br></div><div>On Jun 4, 2011, at 3:39 PM, Chang, Kwang-Poo wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div bgcolor="white" lang="EN-US" link="blue" vlink="blue" id="MailContainerBody" leftmargin="0" topmargin="0" name="Compose message area" canvastabstop="true"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><div class="Section1" style="page: Section1; "><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">I gather from John David and from previous reports about this in the WLs, it is a special device to generate radio wave, producing heat that is slightly above body temperature, but deep penetrating to kill Leishmania in the lesion. Abhay may well be able to provide the device for trials ? He can certainly provide you with a lot more information.<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">KP<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div><div class="MsoNormal" align="center" style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; text-align: center; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><hr size="2" width="100%" align="center" tabindex="-1"></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Sharmanl [<a href="mailto:nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">mailto:nandlals@hotmail.com</a>]<span class="Apple-converted-space"> </span><br><b><span style="font-weight: bold; ">Sent:</span></b><span class="Apple-converted-space"> </span>Saturday, June 04, 2011 12:51 AM<br><b><span style="font-weight: bold; ">To:</span></b><span class="Apple-converted-space"> </span>Satoskar, Abhay; Chang, Kwang-Poo; Raj; Petr Volf;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a>;<span class="Apple-converted-space"> </span><a href="mailto:vishwamohan_katoch@yahoo.co.in" style="color: blue; text-decoration: underline; ">vishwamohan_katoch@yahoo.co.in</a><br><b><span style="font-weight: bold; ">Subject:</span></b><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry</span></font><o:p></o:p></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Dear Dr. Satoskar,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Thanks for this piece of information and also the reprint. However, I have some questions regarding this. What exactly the Radiofrequency heat therapy means , Exactly what type of instrument is this . We do have dermatological radiofrequency cautery machines, is it the same; but here the electrodes are there which cut / coagulate the tissue. I am not aware of any appliance in our set up which creates field generating heat/ temperature in a granulamatous tissue of the size say few cms. I would surely like to know.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Thanks once again</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">NLS</span></font> <o:p></o:p></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><o:p> </o:p></span></font></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:Abhay.Satoskar@osumc.edu" title="Abhay.Satoskar@osumc.edu" style="color: blue; text-decoration: underline; ">Satoskar, Abhay</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Saturday, June 04, 2011 4:19 AM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">To:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">'Sharman'</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu" style="color: blue; text-decoration: underline; ">Chang, Kwang-Poo</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au" style="color: blue; text-decoration: underline; ">Raj</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz" style="color: blue; text-decoration: underline; ">Petr Volf</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" title="mailto:leish-l@lineu.icb.usp.br CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" title="hgoto@usp.br" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" title="elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:vishwamohan_katoch@yahoo.co.in" title="vishwamohan_katoch@yahoo.co.in" style="color: blue; text-decoration: underline; ">vishwamohan_katoch@yahoo.co.in</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>RE: [Leish-l] inquiry<o:p></o:p></span></font></div></div></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="blue" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: blue; ">Dear Dr. Sharma:</span></font><o:p></o:p></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="blue" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: blue; ">I would be interested in know how you are treating your CL patients? I presume intralesional SSG.</span></font><o:p></o:p></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="blue" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: blue; ">Would you be interested in using radio-frequency induced topical heat therapy which we have found to effective even in SSG non-responsive cases of CL in<span class="Apple-converted-space"> </span><st1:country-region w:st="on"><st1:place w:st="on">India</st1:place></st1:country-region>. I am enclosing a reprint of a paper for your information. Let me know</span></font><o:p></o:p></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="blue" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: blue; ">Thanks</span></font><o:p></o:p></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="blue" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: blue; ">Abhay</span></font><o:p></o:p></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><p style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Abhay R. Satoskar M.D., Ph.D.</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Professor</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Departments of Pathology and Microbiology</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Starling Loving Hall M418</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">The<span class="Apple-converted-space"> </span><st1:placename w:st="on">Ohio</st1:placename><span class="Apple-converted-space"> </span><st1:placetype w:st="on">State</st1:placetype><span class="Apple-converted-space"> </span><st1:placetype w:st="on">University</st1:placetype></span></font><span class="Apple-converted-space"> </span><br><st1:place w:st="on"><st1:city w:st="on"><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Columbus</span></font></st1:city><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">,<span class="Apple-converted-space"> </span><st1:state w:st="on">Ohio</st1:state></span></font></st1:place><span class="Apple-converted-space"> </span><br><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Tel: 614-366-3417</span></font><o:p></o:p></p><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div><div class="MsoNormal" align="center" style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; text-align: center; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><hr size="2" width="100%" align="center" tabindex="-1"></span></font></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 12pt; "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:leish-l-bounces@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l-bounces@lineu.icb.usp.br</a><span class="Apple-converted-space"> </span>[<a href="mailto:leish-l-bounces@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">mailto:leish-l-bounces@lineu.icb.usp.br</a>]<span class="Apple-converted-space"> </span><b><span style="font-weight: bold; ">On Behalf Of<span class="Apple-converted-space"> </span></span></b>Sharman<br><b><span style="font-weight: bold; ">Sent:</span></b><span class="Apple-converted-space"> </span>Friday, June 03, 2011 12:15 PM<br><b><span style="font-weight: bold; ">To:</span></b><span class="Apple-converted-space"> </span>Chang, Kwang-Poo; Raj; Petr Volf;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a>;<span class="Apple-converted-space"> </span><a href="mailto:vishwamohan_katoch@yahoo.co.in" style="color: blue; text-decoration: underline; ">vishwamohan_katoch@yahoo.co.in</a><br><b><span style="font-weight: bold; ">Subject:</span></b><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry</span></font><o:p></o:p></p><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Dear KP,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Yes you remember correctly that these strains are different from common Indian Strain of L. donovani of<span class="Apple-converted-space"> </span><st1:place w:st="on">Bihar</st1:place>. The three strains examined at<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:placename w:st="on">London</st1:placename><span class="Apple-converted-space"> </span><st1:placetype w:st="on">School</st1:placetype></st1:place><span class="Apple-converted-space"> </span>of tropical Medicine with each having different homologies but they were also not L infantum. However, some other strains examined at<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:placename w:st="on">Rosalind</st1:placename><span class="Apple-converted-space"> </span><st1:placename w:st="on">franklin</st1:placename><span class="Apple-converted-space"> </span><st1:placetype w:st="on">University</st1:placetype></st1:place><span class="Apple-converted-space"> </span>had results which you are mentioning. We had two dogs positive for rK 39 and one which could follow, died after about 5 months.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Dr. Sunil Arora had also experience of handling these strains but also could not maintain for long.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">NL Sharma</span></font> <o:p></o:p></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><o:p> </o:p></span></font></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="mailto:KwangPoo.Chang@rosalindfranklin.edu CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">Chang, Kwang-Poo</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Thursday, June 02, 2011 11:40 PM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">To:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">Sharman</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au" style="color: blue; text-decoration: underline; ">Raj</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz" style="color: blue; text-decoration: underline; ">Petr Volf</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" title="mailto:leish-l@lineu.icb.usp.br CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" title="mailto:hgoto@usp.br CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" title="elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:vishwamohan_katoch@yahoo.co.in" title="mailto:vishwamohan_katoch@yahoo.co.in CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">vishwamohan_katoch@yahoo.co.in</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>RE: [Leish-l] inquiry<o:p></o:p></span></font></div></div></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">Dear Dr. Sharma,<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">Is the L donovani in the<span class="Apple-converted-space"> </span><st1:placename w:st="on">Satluj</st1:placename><span class="Apple-converted-space"> </span><st1:placetype w:st="on">River</st1:placetype><span class="Apple-converted-space"> </span><st1:placetype w:st="on">Valley</st1:placetype><span class="Apple-converted-space"> </span>different from the L donovani in<span class="Apple-converted-space"> </span><st1:place w:st="on">Bihar</st1:place><span class="Apple-converted-space"> </span>? This is an important point.<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">The genetic site we examined did reveal identity of your samples with L infantum, but differs from L donovani from Bihar and<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">Sudan</st1:country-region></st1:place>. Have you not found rK39+ dogs ? In one of your samples, we saw evidence of both L infantum and L tropica. Whether this is a hybrid or a mixture of the two cannot be certain.<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">It would be good if Petr Volf could have an opportunity of looking into the sand flies there ?<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; ">KP<o:p></o:p></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="navy" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: navy; "><o:p> </o:p></span></font></div><div><div class="MsoNormal" align="center" style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; text-align: center; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><hr size="2" width="100%" align="center" tabindex="-1"></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Sharman [<a href="mailto:nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">mailto:nandlals@hotmail.com</a>]<span class="Apple-converted-space"> </span><br><b><span style="font-weight: bold; ">Sent:</span></b><span class="Apple-converted-space"> </span>Wednesday, June 01, 2011 11:40 AM<br><b><span style="font-weight: bold; ">To:</span></b><span class="Apple-converted-space"> </span>Raj; Petr Volf; Chang, Kwang-Poo;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a>;<span class="Apple-converted-space"> </span><a href="mailto:vishwamohan_katoch@yahoo.co.in" style="color: blue; text-decoration: underline; ">vishwamohan_katoch@yahoo.co.in</a><br><b><span style="font-weight: bold; ">Subject:</span></b><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry</span></font><o:p></o:p></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">Dear all,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">The discussion is getting very interesting. To let you know our focus of CL in<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">India</st1:country-region></st1:place>, I.e. Satluj Valley focus has got L donovani predominantly and L . tropica as well. Two Labs conformed L donovani with approximately 4: 1 ratio to L. tropica. Some more samples tested by Dr. Chang revealed L infantum and L tropica. Results of First study were reported in AJTMH in 2005. L.infantum results still unpublished. Another peculiar observation we observed in a child patient was that after injecting SSG intralesionally white spots developed at the injection sites. Whether this was toxicity of SSG or it is a counterpart of PKDL as L. donovani is the cause is a question still to be answered. As I mentioned earlier some of the patients' lesional exudate was also rK 39 positive. Well number of questions unanswered. Will try to find answers.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Calibri"><span style="font-size: 12pt; font-family: Calibri; ">NLS</span></font> <o:p></o:p></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><o:p> </o:p></span></font></div></div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au" style="color: blue; text-decoration: underline; ">Raj</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Wednesday, June 01, 2011 8:07 AM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">To:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz" style="color: blue; text-decoration: underline; ">Petr Volf</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:Kwang-Poo.Chang@rosalindfranklin.edu" title="Kwang-Poo.Chang@rosalindfranklin.edu" style="color: blue; text-decoration: underline; ">Chang, Kwang-Poo</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">nandlals@hotmail.com</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" title="mailto:hgoto@usp.br CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" title="mailto:elfadil_abass@yahoo.com CTRL + Click to follow link" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(245, 245, 245); "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry<o:p></o:p></span></font></div></div></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "> <st1:street w:st="on"><st1:address w:st="on">G'Day Dr</st1:address></st1:street><span class="Apple-converted-space"> </span>Petr Volf,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Thank you very much for your paper. Excellent molecular typing of parasites.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">As you see my correspondence below, we have a antibody ELISA where we use exoantigens from individual<span class="Apple-converted-space"> </span><em><i><font face="Arial"><span style="font-family: Arial; ">L. infantum</span></font></i></em><span class="Apple-converted-space"> </span>and<span class="Apple-converted-space"> </span><em><i><font face="Arial"><span style="font-family: Arial; ">L. tropica</span></font></i></em>. </span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "> If you are interested I can test plasma/serum samples for presence of antibody response. If so please let me know.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Raj</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Cellabs</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><st1:street w:st="on"><st1:address w:st="on"><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">G'Day Dr</span></font></st1:address></st1:street><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Hanafi,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">My enquiry was to know whether<span class="Apple-converted-space"> </span><em><i><font face="Arial"><span style="font-family: Arial; ">L. donovani</span></font></i></em><span class="Apple-converted-space"> </span>in<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">India</st1:country-region></st1:place><span class="Apple-converted-space"> </span>produces a cutaneous form of lesion or not (I am not referring to PKDL form). There are many reports from SriLanka about the cutaneous manifestations by <em><i><font face="Arial"><span style="font-family: Arial; ">L. donovani</span></font></i></em>. The PKDL although is a cutaneous response appears very late in the infection process and this is ascribed to be a protective response against the parasite.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Prof Chang mentioned</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; ">"Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">Hatay</st1:city>,<span class="Apple-converted-space"> </span><st1:country-region w:st="on">Turkey</st1:country-region></st1:place>." This is from<span class="Apple-converted-space"> </span><st1:place w:st="on">Sutlej</st1:place><span class="Apple-converted-space"> </span>region." It means this cutaneous complex requires some investigation.<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; ">I raise species-specific exo-antigens from<span class="Apple-converted-space"> </span><em><i><font face="Times New Roman">L. tropica</font></i></em><span class="Apple-converted-space"> </span>and<span class="Apple-converted-space"> </span><em><i><font face="Times New Roman">L infantum</font></i></em><span class="Apple-converted-space"> </span>and I am prepared to test the serum /plasma samples for presence of antibodies. If Dr NL Sharma is prepared to send me specimens as well as clinical plasma or serum samples I would be interested to investigate the antibody response in these situations.<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Dr Raj</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Cellabs</span></font><o:p></o:p></div></div><blockquote style="border-top-style: none; border-right-style: none; border-bottom-style: none; border-width: initial; border-color: initial; border-left-style: solid; border-left-color: black; border-left-width: 1pt; padding-top: 0in; padding-right: 0in; padding-bottom: 0in; padding-left: 3pt; margin-left: 2.5pt; margin-top: 5pt; margin-right: 0in; margin-bottom: 5pt; "><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">----- Original Message -----<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(228, 228, 228); "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">From:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz" style="color: blue; text-decoration: underline; ">Petr Volf</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">To:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:raj@cellabs.com.au" title="raj@cellabs.com.au" style="color: blue; text-decoration: underline; ">Raj</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Tuesday, May 31, 2011 5:08 PM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Fw: [Leish-l] inquiry<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Dear Dr Raj,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">I saw your question to Dr. Sharma. I am forwarding my response to him as well the paper which might be interesting also for you.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Best wishes</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Petr Volf</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">----- Original Message -----<o:p></o:p></span></font></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(228, 228, 228); "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">From:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:volf@cesnet.cz" title="volf@cesnet.cz" style="color: blue; text-decoration: underline; ">Petr Volf</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">To:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu" style="color: blue; text-decoration: underline; ">Chang, Kwang-Poo</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">Sharman</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" title="hgoto@usp.br" style="color: blue; text-decoration: underline; ">Hiro Goto</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" title="elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil abass</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Cc:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Tuesday, May 31, 2011 9:06 AM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry<o:p></o:p></span></font></div></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Hi K.P.,</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">in Cukurova region,<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">Turkey</st1:country-region></st1:place>, cutaneous L. infantum (now it seems that we are dealing with L.donovani/L. infantum hybrid) grew very poorely if we isolated them from patients. Only 1 of 25 isolations was succesfull. However, the same strain (confirmed by molecular methods) grew repeatedly and very well if we isolated them from sandflies. It might be useful for Dr. Sharma try to get isolates from sand flies. It is very laborious but very useful, in Cukurova we got about dozen of isolates by this method (all identical).</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Patients are rK39 negative, see attached paper.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Best wishes</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Petr</span></font><o:p></o:p></div></div><blockquote style="border-top-style: none; border-right-style: none; border-bottom-style: none; border-width: initial; border-color: initial; border-left-style: solid; border-left-color: black; border-left-width: 1pt; padding-top: 0in; padding-right: 0in; padding-bottom: 0in; padding-left: 3pt; margin-left: 2.5pt; margin-top: 5pt; margin-right: 0in; margin-bottom: 5pt; "><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">----- Original Message -----<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: rgb(228, 228, 228); "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">From:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:KwangPoo.Chang@rosalindfranklin.edu" title="KwangPoo.Chang@rosalindfranklin.edu" style="color: blue; text-decoration: underline; ">Chang, Kwang-Poo</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">To:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:nandlals@hotmail.com" title="nandlals@hotmail.com" style="color: blue; text-decoration: underline; ">Sharman</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:hgoto@usp.br" title="hgoto@usp.br" style="color: blue; text-decoration: underline; ">Hiro Goto</a><span class="Apple-converted-space"> </span>;<span class="Apple-converted-space"> </span><a href="mailto:elfadil_abass@yahoo.com" title="elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil abass</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Cc:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" title="leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Sent:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Sunday, May 22, 2011 1:39 AM<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; font-weight: bold; ">Subject:</span></font></b><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; "><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry<o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><div id="idOWAReplyText41530"><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" color="black" face="Arial"><span style="font-size: 10pt; font-family: Arial; color: black; ">Dr. NL Sharma has been working on an important CL endemic area along the<span class="Apple-converted-space"> </span><st1:placename w:st="on">Satluj</st1:placename><span class="Apple-converted-space"> </span><st1:placetype w:st="on">River</st1:placetype><span class="Apple-converted-space"> </span>valley to the south of Himalaya in<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">India</st1:country-region></st1:place><span class="Apple-converted-space"> </span>(Please correct me should I be wrong for anything I said here and below). I had the good fortune of visiting the site several years back courtesy of Dr. Sharma.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">I believe Dr. Sharma's finding is important, since the parasites there are different from the familiar Indian L donovani in<span class="Apple-converted-space"> </span><st1:place w:st="on">Bihar</st1:place>. The parasites are refratory to in vitro cultivation. They do differentiate into promastigotes and may grow a little, but can't really be subcultured to establish passageable lines. This is very much reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I remember correctly. If so, Satluj river valley is endemic to the infantile CL.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">Hatay</st1:city>,<span class="Apple-converted-space"> </span><st1:country-region w:st="on">Turkey</st1:country-region></st1:place>.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Arial"><span style="font-size: 12pt; font-family: Arial; ">These observations make me wonder a lot about our current knowledge on the clinico-epidemiology based on data collected previously by analyses of cultured promastigotes from one or few 'representative samples'. Nowaday, technology makes it very doable to work with biological samples for Leish DNAs directly from sand flies, patients and reservoir animals.</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="2" face="Arial"><span style="font-size: 10pt; font-family: Arial; ">KP</span></font><o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div></div><div id="idSignature79278"><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "> <o:p></o:p></span></font></div></div><div><div class="MsoNormal" align="center" style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; text-align: center; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><hr size="2" width="100%" align="center" tabindex="-1"></span></font></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 12pt; "><b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; font-weight: bold; ">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size: 10pt; font-family: Tahoma; "><span class="Apple-converted-space"> </span><a href="mailto:leish-l-bounces@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l-bounces@lineu.icb.usp.br</a><span class="Apple-converted-space"> </span>on behalf of Sharman<br><b><span style="font-weight: bold; ">Sent:</span></b><span class="Apple-converted-space"> </span>Fri 5/20/2011 11:31 PM<br><b><span style="font-weight: bold; ">To:</span></b><span class="Apple-converted-space"> </span>Hiro Goto; elfadil abass<br><b><span style="font-weight: bold; ">Cc:</span></b><span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><br><b><span style="font-weight: bold; ">Subject:</span></b><span class="Apple-converted-space"> </span>Re: [Leish-l] inquiry</span></font><o:p></o:p></p></div></div><div><p style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; "><font size="2" face="Times New Roman"><span style="font-size: 10pt; ">Dear all<br>I agree with Hiro gito, We are working on a focus where the CL is<br>predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE<br>RESULTS WITH SERA as well as serous exudate from the lesion. The results are<br>dependent upon species.<br>NL Sharma<br><br>--------------------------------------------------<br>From: "Hiro Goto" <<a href="mailto:hgoto@usp.br" style="color: blue; text-decoration: underline; ">hgoto@usp.br</a>><br>Sent: Wednesday, May 18, 2011 12:09 AM<br>To: "elfadil abass" <<a href="mailto:elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a>><br>Cc: <<a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>><br>Subject: Re: [Leish-l] inquiry<br><br>> Dear all,<br>> In our oppinion, DAT and rK39 for those samples are not indicated since<br>> these tests are produced for the diagnosis of visceral leishmaniasis.<br>> In case of tegumentary leishmaniasis, it is very appropriate the<br>> observation of J.J. Shaw appointing species specificity of antibody<br>> response in these cases. Titers of antibodies are in general low in these<br>> cases therefore depending on the species, it may result negative. We have<br>> published a review recently in Expert Rev. Anti Infect. Ther. 8(4),<br>> 419?433 (2010), Current diagnosis and treatment of cutaneous and<br>> mucocutaneous leishmaniasis, where we raise this point.<br>> Hiro Goto<br>><br>> Citando elfadil abass <<a href="mailto:elfadil_abass@yahoo.com" style="color: blue; text-decoration: underline; ">elfadil_abass@yahoo.com</a>>:<br>><br>>> Dear all I would recommend using DAT and rK39 strip test to measure<br>>> antibody<br>>> responses and to evaluate the diagnostic efficiency for both tests in<br>>> such group<br>>> of patients.<br>>><br>>> Elfadil Abass<br>>> ________________________________________________________________________________<br>>><span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:placetype w:st="on">Institute</st1:placetype><span class="Apple-converted-space"> </span>of<span class="Apple-converted-space"> </span><st1:placename w:st="on">Medical</st1:placename></st1:place><span class="Apple-converted-space"> </span>Microbiology and Hospital Hygiene<br>>><span class="Apple-converted-space"> </span><st1:placename w:st="on">Philipps</st1:placename><span class="Apple-converted-space"> </span><st1:placename w:st="on">University</st1:placename><span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">Marburg</st1:city></st1:place><br>>> BMFZ / Hans-Meerwein Straße 2<br>>> D-35033<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">Marburg</st1:city>,<span class="Apple-converted-space"> </span><st1:country-region w:st="on">Germany</st1:country-region></st1:place><br>>> ________________________________________________________________________________<br>>><br>>><br>>><br>>><br>>> ________________________________<br>>> From: Nuha Nuwayri-Salti <<a href="mailto:racha@aub.edu.lb" style="color: blue; text-decoration: underline; ">racha@aub.edu.lb</a>><br>>> To: saad saad <<a href="mailto:saad1426@gmail.com" style="color: blue; text-decoration: underline; ">saad1426@gmail.com</a>>; "<a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>"<br>>> <<a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a>><br>>> Sent: Fri, May 13, 2011 9:39:33 AM<br>>> Subject: Re: [Leish-l] inquiry<br>>><br>>> Dear Saad first precaution to take is to separate your samples into<br>>> several<br>>> portions each (at least 5 each being no more than a few hundred(200-400)<br>>> microliters). This is a necessary precaution to avoid freezing and<br>>> thawing<br>>> several times the same sample which will be the case should you do<br>>> different<br>>> studies at different times which is unavoidable.<br>>><br>>> What you can do is correlate the type(ulcerated, abscess, furuncle<br>>> etc.. ) the<br>>> locale, the number and age of lesions with the levels of antibody in the<br>>> sera of<br>>> these patgients and also monitor cell mediated immunity with leishmanin<br>>> skin<br>>> test!<br>>> I have just published (in print) an article about having circulating<br>>> parasites<br>>> in some of these patients with apparently pure cutaneous disease. It is<br>>> the<br>>> first paper that revealed this fact. It would be interesting to confirm<br>>> or<br>>> de-confirm this fact repeating what we did.<br>>> Best wishes<br>>><br>>> Nuha Nuwayri-Salti MD<br>>><br>>><br>>><br>>> AOA<br>>> Medical Honor Society<br>>><br>>><br>>><br>>><br>>> -----Original Message-----<br>>> From:<span class="Apple-converted-space"> </span><a href="mailto:leish-l-bounces@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l-bounces@lineu.icb.usp.br</a><br>>> [<a href="mailto:leish-l-bounces@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">mailto:leish-l-bounces@lineu.icb.usp.br</a>]<br>>> On Behalf Of saad saad<br>>> Sent: Friday, April 29, 2011 11:23 PM<br>>> To:<span class="Apple-converted-space"> </span><a href="mailto:leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">leish-l@lineu.icb.usp.br</a><br>>> Subject: [Leish-l] inquiry<br>>><br>>> Dear all<br>>> Hi. i am Saad from<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:country-region w:st="on">Saudi Arabia</st1:country-region></st1:place>. i have 60 sera from positive case of<br>>> CL from south west of the country. I would like to have your<br>>> recommendations to start a good research line in CL using these sera.<br>>> Thanks in advance for your help<br>>> _______________________________________________<br>>> Leish-l mailing list<br>>><span class="Apple-converted-space"> </span><a href="mailto:Leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">Leish-l@lineu.icb.usp.br</a><br>>><span class="Apple-converted-space"> </span><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" style="color: blue; text-decoration: underline; ">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>>> _______________________________________________<br>>> Leish-l mailing list<br>>><span class="Apple-converted-space"> </span><a href="mailto:Leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">Leish-l@lineu.icb.usp.br</a><br>>><span class="Apple-converted-space"> </span><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" style="color: blue; text-decoration: underline; ">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>>><br>><br>><br>><br>> Profa. Dra. Hiro Goto<br>> Laboratório de Soroepidemiologia e Imunobiologia<br>> Instituto de Medicina Tropical de<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">São Paulo</st1:city></st1:place>, USP<br>> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar<br>> 05403-000 -<span class="Apple-converted-space"> </span><st1:place w:st="on"><st1:city w:st="on">São Paulo</st1:city></st1:place>, SP<br>> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027<br>> Fax. +55-11-3061-8270<br>><br>> _______________________________________________<br>> Leish-l mailing list<br>><span class="Apple-converted-space"> </span><a href="mailto:Leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">Leish-l@lineu.icb.usp.br</a><br>><span class="Apple-converted-space"> </span><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" style="color: blue; text-decoration: underline; ">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><br>><br>_______________________________________________<br>Leish-l mailing list<br><a href="mailto:Leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">Leish-l@lineu.icb.usp.br</a><br><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" style="color: blue; text-decoration: underline; ">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a></span></font><o:p></o:p></p></div><div class="MsoNormal" align="center" style="margin-top: 0in; margin-right: 0in; margin-bottom: 0.0001pt; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; text-align: center; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><hr size="2" width="100%" align="center"></span></font></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0in; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; ">_______________________________________________<br>Leish-l mailing list<br><a href="mailto:Leish-l@lineu.icb.usp.br" style="color: blue; text-decoration: underline; ">Leish-l@lineu.icb.usp.br</a><br><a href="http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l" style="color: blue; text-decoration: underline; ">http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l</a><o:p></o:p></span></font></div></blockquote><div style="margin-right: 0in; 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