[leish-l] Typing Leishmania

Tue Sep 20 13:35:11 BRT 2005

Re Dick's comments on identification of Leishmania, we do have a 'widely 
accepted molecular method for describing individual infections so that they 
can be identified according to a reliable classification. ' It's called 
isoenzyme typing!
Bob Killick-Kendrick

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>   1. Re: Re; Leishmania in Antigua (Richard Ashford)
>
> --__--__--
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> Message: 1
> Date: Sun, 18 Sep 2005 08:44:38 +0100
> From: Richard Ashford <ashford at liverpool.ac.uk>
> To: "Arias, Jorge Dr." <Jorge.Arias at fairfaxcounty.gov>,
> leish-l at fat.org.br
> Subject: Re: [leish-l] Re; Leishmania in Antigua
>
> Dear all,
>
> Interesting correspondence on the supposed Antiguan leish case.  This
> highlights the problem of having no widely accepted molecular method for
> describing individual infections so that they can be identified according
> to a reliable classification.  This is where we came in, of course, some
> thirty, or even seventy years ago.  I wonder what 'the biopsies have a
> positive PCR for L mexicana sp complex' actually means.  Is it that the 
> PCR
> primers used specifically amplify bits of L. mexicana only, so that any
> product must be L. mexicana 'sp complex', or is it that the product 
> reacted
> with specific probes, whether DNA or monoclonal probes?  Or is that my
> understanding is stuck in the dark ages?
>
> I agree, and have often stated, that monoclonals and DNA probes may be 
> very
> valuable for eco/epidemiological work in circumstances where the range of
> parasites present is well understood.  But for aberrant cases or cases 
> from
> special places, full, standardised enzyme description is essential before
> any useful answer can be suggested.  I wonder if any parasites were even
> seen in Rob's case?
>
> Go well,
>
> Dick
>
>
>
> --On 16 September 2005 11:11 -0400 "Arias, Jorge Dr."
> <Jorge.Arias at fairfaxcounty.gov> wrote:
>
>> I agree with Bob Killick Kendrick in that there are no official reports
>> of either sandflies or leishmaniasis from Antigua. If the patients stay
>> on the island was brief, and it did not include the extra-urban area of
>> the island, I would pursue more travel history (Yucatan Peninsula?
>> Cancun, Cozumel, Belize?) even if it was in a previous trip in the last
>> year. There is a _mexicana_ complex parasite in the Dominican Republic
>> that causes DCL, but it is not know to cause CL.
>> In the event there is something new out there that hasn't come to light
>> yet, it may be worthwhile to contact Chris Frederickson (CAREC) at
>> frederch at carec.paho.org to see if he has seen anything new reported.
>> Keep us posted, it is interesting.
>> Good luck,
>>
>> Jorge R. Arias Ph.D.
>>
>>
>>
>> -----Original Message-----
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>> Behalf Of leish-l-request at fat.org.br
>> Sent: Friday, September 16, 2005 11:01 AM
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>> Subject: leish-l digest, Vol 1 #250 - 1 msg
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>>
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>>    1. Antigua & leishmaniasis (BobKillick-Kendrick)
>>
>> -- __--__-- 
>>
>> Message: 1
>> From: "BobKillick-Kendrick" <killickendrick at wanadoo.fr>
>> To: <leish-l at fat.org.br>
>> Date: Thu, 8 Sep 2005 11:48:16 +0200
>> Subject: [leish-l] Antigua & leishmaniasis
>>
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>> There is no mention of leishmaniasis in Antigua in the comprehensive =
>> review by Arias et al. (1996) [Epidemiologia y control de la =
>> leishmaniasis en las Am=E9ricas, por pais o territorio.OPS, Cuaderno =
>> T=E9cnico 44]. (Google this to see it on line.) Furthermore, there are =
>> no records of phlebotomine sand flies on Antigua in the most up-to-date
>> = book on American phlebotomines [Young & Duncan, 1994.] But this does
>> not = mean there is no leishmaniasis on Antigua. Probably nobody has
>> looked = for sand flies there, and L. mexicana might be on the island in
>> forest = rodents with no human cases yet recorded - as in Trinidad.
>> Confirmation = of the identity of the parasite seems necessary -
>> preferably by = isoenzymes(?)=20 Bob Killick-Kendrick
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>
>
>
> R.W. Ashford
> Consultant Biologist
> Professor (Retired) of Parasite and Vector Biology
> c/o Liverpool School of Tropical Medicine
> Liverpool L3 5QA
> Tel: +44 151 632 2714
> Fax: +44 151 705 3371
> e-mail: ashford at liv.ac.uk
>
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