[leish-l] Typing Leishmania

Thu Sep 22 14:19:18 BRT 2005

Thank you, Bob, for your perspicacity - in fact, as shown by my student 
Manal Jamjoom, the enzyme systems for 'typing' (= describing and 
identifying)Leish isolates breaks down in the donovani complex, which is 
why she developed a battery of microsatellites.  Further, there is great 
incompatibility between the European and US results.  We're a bit further 
forward than thirty years ago, but not as much as we should be.

All the best,

Dick

--On 20 September 2005 18:35 +0200 BobKillick-Kendrick 
<killickendrick at wanadoo.fr> wrote:

> Re Dick's comments on identification of Leishmania, we do have a 'widely
> accepted molecular method for describing individual infections so that
> they can be identified according to a reliable classification. ' It's
> called isoenzyme typing!
> Bob Killick-Kendrick
>
> ----- Original Message ----- From: <leish-l-request at fat.org.br>
> To: <leish-l at fat.org.br>
> Sent: Tuesday, September 20, 2005 5:01 PM
> Subject: leish-l digest, Vol 1 #252 - 1 msg
>
>
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>>   1. Re: Re; Leishmania in Antigua (Richard Ashford)
>>
>> --__--__--
>>
>> Message: 1
>> Date: Sun, 18 Sep 2005 08:44:38 +0100
>> From: Richard Ashford <ashford at liverpool.ac.uk>
>> To: "Arias, Jorge Dr." <Jorge.Arias at fairfaxcounty.gov>,
>> leish-l at fat.org.br
>> Subject: Re: [leish-l] Re; Leishmania in Antigua
>>
>> Dear all,
>>
>> Interesting correspondence on the supposed Antiguan leish case.  This
>> highlights the problem of having no widely accepted molecular method for
>> describing individual infections so that they can be identified according
>> to a reliable classification.  This is where we came in, of course, some
>> thirty, or even seventy years ago.  I wonder what 'the biopsies have a
>> positive PCR for L mexicana sp complex' actually means.  Is it that the
>> PCR
>> primers used specifically amplify bits of L. mexicana only, so that any
>> product must be L. mexicana 'sp complex', or is it that the product
>> reacted
>> with specific probes, whether DNA or monoclonal probes?  Or is that my
>> understanding is stuck in the dark ages?
>>
>> I agree, and have often stated, that monoclonals and DNA probes may be
>> very
>> valuable for eco/epidemiological work in circumstances where the range of
>> parasites present is well understood.  But for aberrant cases or cases
>> from
>> special places, full, standardised enzyme description is essential before
>> any useful answer can be suggested.  I wonder if any parasites were even
>> seen in Rob's case?
>>
>> Go well,
>>
>> Dick
>>
>>
>>
>> --On 16 September 2005 11:11 -0400 "Arias, Jorge Dr."
>> <Jorge.Arias at fairfaxcounty.gov> wrote:
>>
>>> I agree with Bob Killick Kendrick in that there are no official reports
>>> of either sandflies or leishmaniasis from Antigua. If the patients stay
>>> on the island was brief, and it did not include the extra-urban area of
>>> the island, I would pursue more travel history (Yucatan Peninsula?
>>> Cancun, Cozumel, Belize?) even if it was in a previous trip in the last
>>> year. There is a _mexicana_ complex parasite in the Dominican Republic
>>> that causes DCL, but it is not know to cause CL.
>>> In the event there is something new out there that hasn't come to light
>>> yet, it may be worthwhile to contact Chris Frederickson (CAREC) at
>>> frederch at carec.paho.org to see if he has seen anything new reported.
>>> Keep us posted, it is interesting.
>>> Good luck,
>>>
>>> Jorge R. Arias Ph.D.
>>>
>>>
>>>
>>> -----Original Message-----
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>>> Behalf Of leish-l-request at fat.org.br
>>> Sent: Friday, September 16, 2005 11:01 AM
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>>> Subject: leish-l digest, Vol 1 #250 - 1 msg
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>>>
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>>> Today's Topics:
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>>>    1. Antigua & leishmaniasis (BobKillick-Kendrick)
>>>
>>> -- __--__--
>>>
>>> Message: 1
>>> From: "BobKillick-Kendrick" <killickendrick at wanadoo.fr>
>>> To: <leish-l at fat.org.br>
>>> Date: Thu, 8 Sep 2005 11:48:16 +0200
>>> Subject: [leish-l] Antigua & leishmaniasis
>>>
>>> This is a multi-part message in MIME format.
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>>>
>>> There is no mention of leishmaniasis in Antigua in the comprehensive =
>>> review by Arias et al. (1996) [Epidemiologia y control de la =
>>> leishmaniasis en las Am=E9ricas, por pais o territorio.OPS, Cuaderno =
>>> T=E9cnico 44]. (Google this to see it on line.) Furthermore, there are =
>>> no records of phlebotomine sand flies on Antigua in the most up-to-date
>>> = book on American phlebotomines [Young & Duncan, 1994.] But this does
>>> not = mean there is no leishmaniasis on Antigua. Probably nobody has
>>> looked = for sand flies there, and L. mexicana might be on the island in
>>> forest = rodents with no human cases yet recorded - as in Trinidad.
>>> Confirmation = of the identity of the parasite seems necessary -
>>> preferably by = isoenzymes(?)=20 Bob Killick-Kendrick
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>>
>>
>>
>> R.W. Ashford
>> Consultant Biologist
>> Professor (Retired) of Parasite and Vector Biology
>> c/o Liverpool School of Tropical Medicine
>> Liverpool L3 5QA
>> Tel: +44 151 632 2714
>> Fax: +44 151 705 3371
>> e-mail: ashford at liv.ac.uk
>>
>>
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R.W. Ashford
Consultant Biologist
Professor (Retired) of Parasite and Vector Biology
c/o Liverpool School of Tropical Medicine
Liverpool L3 5QA
Tel: +44 151 632 2714
Fax: +44 151 705 3371
e-mail: ashford at liv.ac.uk