[leish-l] Organelle segregation in Leishmania

Thu Mar 25 12:23:57 BRT 2004

Dear Leish -L,

	Larry, as Patrick mentions, probably has only contemporary
publications on this that I'm aware of (Simpson et al., 1974, Simpson and
Kretzner 1997). Keith's lab didn't look at leishmania per se but there are
historic publications on crithidia and possibly also leishmania that people
like Derrick Robinson will be aware of if you want to be sure you haven't
missed anything. Sounds like what you would really like is a mix of the
"Sherwin and Gull 1989" Phil trans paper and the Woodward and Gull 1990 cell
cycle paper for procyclic brucei. If so, this sort of detail has not been
done published for leishmania but if you do undertake it then our new
journal "Kinetoplastid Biology and Disease" would certainly be interested in
reviewing it. Questions about whether all species and life-cycle stages for
leishmania are similar wrt to co-ordination of organelle replication and
segregation are similarly of interest but unresolved as far as I know.

Best regards,
Kevin M. Tyler.

Lecturer in Eukaryotic Microbiology,
School of Medicine, Health Policy and Practice,
University of East Anglia
Norwich, Norfolk NR4 7TJ.

01144(0)1603-591225 (Direct Line)
01144(0)1603-593752 (Fax) 

Kinetoplastid Biology and Disease:
www.kinetoplastids.com/

-----Original Message-----
From: David M. Engman [mailto:d-engman at northwestern.edu] 
Sent: 25 March 2004 13:13
To: k.tyler at uea.ac.uk
Cc: k-toriello at northwestern.edu
Subject: Division in Leishmania

Kevin,

You may have some insight into the division question (see below).  Also 
Krista will develop her autorad today so cross your fingers.

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>From: "K.P. Chang" <changk at mail.finchcms.edu>
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>To: genpara <genpara at univ-montp1.fr>
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>Subject: Re: [leish-l] Mitosis in Leishmania 3-23-04
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>I have no publication records on Leishmania mitosis, but have observed this
>event on numerous occasions for other studies by microscopy. Flagella,
>flagellar pockets and kinetoplasts do seem to divide first, but I can't say
if
>there is any particular order for these three structures. One flagellum is
>always shorter than the other. Nuclear division follows without dissolution
of
>nuclear envelope. Electron microscopy revealed chromatin condensation and
the
>presence of mitotic microtubles. Rarely, one finds anucleated or 
>akinetoplastic
>cells, which presumably result from mitotic errors or abnormality and are
>thought to be non-viable.
>
>KP Chang
>
>genpara wrote:
>
> > Dear all,
> >
> > I am interested in the progression of mitosis and cell division in
> > Leishmania and I cannot remember where I have seen that it was not the
same
> > as in African tryps. You know, the old querella about "kinetoplast
dividing
> > first ? or nucleus first ?". There are loads of articles about brucei
but
> > it sems less clear about Leishmania. Obviously, Larry (Simpson) has
> > analyzed the subject in details (e.g. Simpson & Kretzer 1997) and may be
> > able to answer. But it is often said that "division of the kinetoplast
> > occurs prior to nuclear division in MOST cells" : does that mean that in
> > some cells, it does not ? and that these cells appear as having two
nuclei
> > and one kinetoplast ? In this case, are these cells "normal" or are they
> > experiencing some trouble of their division (hence might not be viable
?).
> > Has anybody seen enough divisions under the microscope to be sure of how
it
> > goes ?
> > Unless there are detailed publications on the subject that have escaped
my
> > attention ?
> > Many thanks for any info.
> > Best wishes
> >
> > Patrick Bastien
> > UMR5093 CNRS/Universit? Montpellier I
> > "Biologie Mol?culaire et G?nome des Protozoaires Parasites"
> > Laboratoire de Parasitologie-Mycologie
> > 163 Rue Auguste Broussonet
> > 34090 Montpellier
> > France
> >
> > Tel: +33 (0)467 63 55 13 or 27 51
> > Fax: +33 (0)467 63 00 49
> > _______________________________________________
> > leish-l mailing list
> > leish-l at fat.org.br
> > http://panda.fat.org.br/mailman/listinfo/leish-l
>
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