[leish-l] Iranian isolates 5-10-03

[Mehdi Mohebali]

Dear Dr.Chang
Thank you for your E.mail.I would like to collaborate with your project 
too.We have recently isolated a few of Leishmania isolates from CL&VL 
infected humans and dogs in different parts of Iran.After mass production of 
promastigotes and DNA extraction ,  a little of them will be send you .
I would very pleased if you help me about their transmission circumstances 
and your accurate address.

With best regards,
Dr.Mehdi Mohebali,(DVM,MPH,Ph.D)
Professor of Medical Parasitology,School of Public Health,Tehran University 
of Medical Sciences.


>From: "K.P. Chang" <changk at mail.finchcms.edu>
>Reply-To: changk at mail.finchcms.edu
>To: Jeffrey Shaw <jeffreyj at usp.br>
>CC: "leish-l-fat.org.br" <leish-l at fat.org.br>, changk at mail.finchcms.edu
>Subject: [leish-l] Iranian isolates 5-10-03
>Date: Sat, 10 May 2003 13:09:26 -0700
>
>To: Dr. Mohebali via Dr. Shaw
>
>We would be happy to examine Leishmania nagt genes. We have used this
>single-copy, highly conserved gene sequences to separate some 20
>haplotypes of Leish isolates. RFLP of PCR-amplified nagt easily separate
>L major, L tropica and L donovani/infantum.
>
>Should there be any interests in this as a collaborative study, send ~2
>ug total genomic DNAs of representative Leish isolates plus all the
>relevant bio information.
>
>In 1997, Dr. Sami Rafati of Tehran kindly sent the following cultures
>from Isfahan to us:
>
>X36 - L tropica
>X54 - L tropica
>X81 - L major
>IR88 - L donovani
>
>It might be interesting to compare the fresh isolates against these.
>
>Looking forward to hearing from you.
>
>Sincerely,
>
>KP Chang
>Dept Microbiol/Immunol
>Univ Heal Sci/Chicago Med School
>3333 Green Bay Rd
>N Chicago, IL 60064, USA
>
>tel 847-578-8837
>
>Jeffrey Shaw wrote:
>
> >
> >
> >
> >> Dear Dr. Mohebali,
> >>
> >> Several years ago the WHO TDR Programme put together a battery of
> >> lypholized reference monoclonal antibodies that were available free
> >> on request. I suggest that you write them and request a set of the
> >> antibodies. They included species specific antibodies for L.
> >> donovani, L. major and L. tropica.
> >>
> >> Yours,
> >>
> >> Charles Jaffe
> >> On Sunday, May 4, 2003, at 21:12 Asia/Jerusalem, Jeffrey Shaw wrote:
> >>
> >>
> >>
> >>
> >> > From: "Mehdi Mohebali" <mmohebali at hotmail.com>
> >> > Subject: Leishmania MAb request
> >> >
> >> >
> >> > Dear Dr.Jeffrey Shaw
> >> >
> >> > My colleaques and I are presently being investigated on Leishmania
> >> > isolations that were collected from Infected humans,animal
> >> > reservoirs and sand fly vectors (CL&VL ) in  endemic foci of  I.R.
> >> > of Iran.We are going to characterize them by molecular techniques
> >> > including on PCR & RAPD-PCR.I think if the isolates   are emphasis
> >> > by appropriate technique  including MAb ,the obtained results will
> >> > be more valid.
> >> > Hereby,I would very appreciated if you  kindly help us about kinds
> >> > of Leishmania MAb and how we can prepare them.
> >> >
> >> > With the best regards,
> >> >
> >> > Dr.Mehdi Mohebali(DVM,MPh,Ph.D)
> >> >
> >> > School of public Health,Tehran University of Medical Sciences.
> >> >
> >>
> >> </blockquote></x-html>
> >
> > _______________________________________________ leish-l mailing list
> > leish-l at fat.org.br http://panda.fat.org.br/mailman/listinfo/leish-l

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