Dr.Mehdi Mohebali [leish-l] Iranian isolates 5-14-03

[K.P. Chang]

Dear Dr. Mohebali,

I am glad to receive your message, indicating that your plan to send us some
Iranian Leishmania DNA for nagt analysis. Please do include all the information
for the sources of the samples, such as the disease, human or animal, place and
year of isolation, previous typing info, culture media used, etc. Also, provide
the DNA concentrations, if known.

I take the liberty of forwarding a copy of this message to Dr. Sami Rafati. I
have not been in touch with her since 1997, but noted her good work on
experimental immunology of leishmaniasis and L major cysteine protease vaccines
from a Pubmed search for her publications (and also her e-mail address).

The nagt sequences of L major and L tropica Dr. Rafati sent to us are identical
to those in the areas, i. e. Pakistan, Iraq, Syria, Uzbekistan, Isreal, Greece,
Afganistan, Turkey and/or S Arabia. However, there are nagt variants of these
two species in the last two countries as well as in Namibia. The sole visceral
isolate, IR88, was sent to us by Professor Edrissian (?). The sample did not
work out technically. The nagt sequences of visceral isolates are interesting.
It is identical for the representatives of L infantum throughout the
Mediterranean basin, in China and L chagasi in Brazil (as expected). Visceral
isolates from Sudan and India have 2 and 4 base substitutions from L infantum,
respectively. Additional visceral isolates from Turkey and China have nagt
sequences, which are very different from L infantum/donovani and unexpectedly
more similar to the cutaneous species. The significance of all these cannot be
known without additional samples for study.

Some information or work in progress are given above just to let you know the
reason why we are interested in nagt from different places. We have other
academic interests in the sequence heterogeneity of this gene as well.

Looking forward to receiving your DNA samples of Iranian isolates.

Sincerely,

KP Chang
Dept Microbiology/Immunology
Univ Health Sci/Chicago Med School
3333 Green Bay Rd
N Chicago, IL 60064, USA

tel 847-578-8837
fax 847-578-3349



Mehdi Mohebali wrote:

> Dear Dr.Chang
> Thank you for your E.mail.I would like to collaborate with your project
> too.We have recently isolated a few of Leishmania isolates from CL&VL
> infected humans and dogs in different parts of Iran.After mass production of
> promastigotes and DNA extraction ,  a little of them will be send you .
> I would very pleased if you help me about their transmission circumstances
> and your accurate address.
>
> With best regards,
> Dr.Mehdi Mohebali,(DVM,MPH,Ph.D)
> Professor of Medical Parasitology,School of Public Health,Tehran University
> of Medical Sciences.
>
> >From: "K.P. Chang" <changk at mail.finchcms.edu>
> >Reply-To: changk at mail.finchcms.edu
> >To: Jeffrey Shaw <jeffreyj at usp.br>
> >CC: "leish-l-fat.org.br" <leish-l at fat.org.br>, changk at mail.finchcms.edu
> >Subject: [leish-l] Iranian isolates 5-10-03
> >Date: Sat, 10 May 2003 13:09:26 -0700
> >
> >To: Dr. Mohebali via Dr. Shaw
> >
> >We would be happy to examine Leishmania nagt genes. We have used this
> >single-copy, highly conserved gene sequences to separate some 20
> >haplotypes of Leish isolates. RFLP of PCR-amplified nagt easily separate
> >L major, L tropica and L donovani/infantum.
> >
> >Should there be any interests in this as a collaborative study, send ~2
> >ug total genomic DNAs of representative Leish isolates plus all the
> >relevant bio information.
> >
> >In 1997, Dr. Sami Rafati of Tehran kindly sent the following cultures
> >from Isfahan to us:
> >
> >X36 - L tropica
> >X54 - L tropica
> >X81 - L major
> >IR88 - L donovani
> >
> >It might be interesting to compare the fresh isolates against these.
> >
> >Looking forward to hearing from you.
> >
> >Sincerely,
> >
> >KP Chang
> >Dept Microbiol/Immunol
> >Univ Heal Sci/Chicago Med School
> >3333 Green Bay Rd
> >N Chicago, IL 60064, USA
> >
> >tel 847-578-8837
> >
> >Jeffrey Shaw wrote:
> >
> > >
> > >
> > >
> > >> Dear Dr. Mohebali,
> > >>
> > >> Several years ago the WHO TDR Programme put together a battery of
> > >> lypholized reference monoclonal antibodies that were available free
> > >> on request. I suggest that you write them and request a set of the
> > >> antibodies. They included species specific antibodies for L.
> > >> donovani, L. major and L. tropica.
> > >>
> > >> Yours,
> > >>
> > >> Charles Jaffe
> > >> On Sunday, May 4, 2003, at 21:12 Asia/Jerusalem, Jeffrey Shaw wrote:
> > >>
> > >>
> > >>
> > >>
> > >> > From: "Mehdi Mohebali" <mmohebali at hotmail.com>
> > >> > Subject: Leishmania MAb request
> > >> >
> > >> >
> > >> > Dear Dr.Jeffrey Shaw
> > >> >
> > >> > My colleaques and I are presently being investigated on Leishmania
> > >> > isolations that were collected from Infected humans,animal
> > >> > reservoirs and sand fly vectors (CL&VL ) in  endemic foci of  I.R.
> > >> > of Iran.We are going to characterize them by molecular techniques
> > >> > including on PCR & RAPD-PCR.I think if the isolates   are emphasis
> > >> > by appropriate technique  including MAb ,the obtained results will
> > >> > be more valid.
> > >> > Hereby,I would very appreciated if you  kindly help us about kinds
> > >> > of Leishmania MAb and how we can prepare them.
> > >> >
> > >> > With the best regards,
> > >> >
> > >> > Dr.Mehdi Mohebali(DVM,MPh,Ph.D)
> > >> >
> > >> > School of public Health,Tehran University of Medical Sciences.
> > >> >
> > >>
> > >> </blockquote></x-html>
> > >
> > > _______________________________________________ leish-l mailing list
> > > leish-l at fat.org.br http://panda.fat.org.br/mailman/listinfo/leish-l
>
> _________________________________________________________________
> The new MSN 8: smart spam protection and 2 months FREE*
> http://join.msn.com/?page=features/junkmail