[leish-l] Iranian isolates 5-10-03

[K.P. Chang]

To: Dr. Mohebali via Dr. Shaw

We would be happy to examine Leishmania nagt genes. We have used this
single-copy, highly conserved gene sequences to separate some 20
haplotypes of Leish isolates. RFLP of PCR-amplified nagt easily separate
L major, L tropica and L donovani/infantum.

Should there be any interests in this as a collaborative study, send ~2
ug total genomic DNAs of representative Leish isolates plus all the
relevant bio information.

In 1997, Dr. Sami Rafati of Tehran kindly sent the following cultures
from Isfahan to us:

X36 - L tropica
X54 - L tropica
X81 - L major
IR88 - L donovani

It might be interesting to compare the fresh isolates against these.

Looking forward to hearing from you.

Sincerely,

KP Chang
Dept Microbiol/Immunol
Univ Heal Sci/Chicago Med School
3333 Green Bay Rd
N Chicago, IL 60064, USA

tel 847-578-8837

Jeffrey Shaw wrote:

>
>
>
>> Dear Dr. Mohebali,
>>
>> Several years ago the WHO TDR Programme put together a battery of
>> lypholized reference monoclonal antibodies that were available free
>> on request. I suggest that you write them and request a set of the
>> antibodies. They included species specific antibodies for L.
>> donovani, L. major and L. tropica.
>>
>> Yours,
>>
>> Charles Jaffe
>> On Sunday, May 4, 2003, at 21:12 Asia/Jerusalem, Jeffrey Shaw wrote:
>>
>>
>>
>>
>> > From: "Mehdi Mohebali" <mmohebali at hotmail.com>
>> > Subject: Leishmania MAb request
>> >
>> >
>> > Dear Dr.Jeffrey Shaw
>> >
>> > My colleaques and I are presently being investigated on Leishmania
>> > isolations that were collected from Infected humans,animal
>> > reservoirs and sand fly vectors (CL&VL ) in  endemic foci of  I.R.
>> > of Iran.We are going to characterize them by molecular techniques
>> > including on PCR & RAPD-PCR.I think if the isolates   are emphasis
>> > by appropriate technique  including MAb ,the obtained results will
>> > be more valid.
>> > Hereby,I would very appreciated if you  kindly help us about kinds
>> > of Leishmania MAb and how we can prepare them.
>> >
>> > With the best regards,
>> >
>> > Dr.Mehdi Mohebali(DVM,MPh,Ph.D)
>> >
>> > School of public Health,Tehran University of Medical Sciences.
>> >
>>
>> </blockquote></x-html>
>
> _______________________________________________ leish-l mailing list
> leish-l at fat.org.br http://panda.fat.org.br/mailman/listinfo/leish-l
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lineu.icb.usp.br/pipermail/leish-l/attachments/20030510/aa527d86/attachment.html