[Leish-l] Status of PCR for CL diagnosis

[Fernando Andrade Narvaez]

Dear Hugo,
You have received several very good answers. Although microscopy remains as
the standard procedure, I think by availability, there is a consensus that
PCR offers advantages for diagnosis and parasite identification genus and
subgenus and not necessarily just in case of chronic cases of LC. Here in
southern Mexico an endemic area of LC by* L. mexicana* we are using mostly
PCR. Isolation-culture are useful for parasitological studies.
Best regards,

Dr. Fernando J. Andrade Narváez
Jefe del Laboratorio de Inmunología
Centro de Investigaciones Regionales "Dr. Hideyo Noguchi"
Universidad Autónoma de Yucatán
Ave. Itzáes No. 490x59 CP 97000
Mérida, Yucatán, México
Tel.: 999 924-6412 ext. 1156 y 1155
Fax: 999 923-6120
E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com

Fernando J. Andrade-Narvaez, MD, PhD
Head of Immunology Laboratory
Centro de Investigaciones Regionales "Dr. Hideyo Noguchi"
Universidad Autónoma de Yucatán,
Ave. Itzáes No. 490x59 CP 97000
Mérida, Yucatán, México
Tel.: 52 999 924-6412 ext. 1156 & 1155
Fax: 52 999 923-6120
E-mails: anarvaez at correo.uady.mx, fandrade47 at gmail.com




2017-03-26 22:30 GMT-06:00 Hugo Valdivia <hvalrod at hotmail.com>:

> Dear colleagues,
>
> We have been recently questioned about the use of PCR for CL  diagnosis
> given that the Peruvian Ministry of health considers microscopy as the
> standard practice for CL diagnosis. In this sense, I would like to hear
> your thoughts about the use of PCR for CL diagnosis, specially from those
> colleagues working in reference diagnostic centers. What I reviewed made me
> think that there is no a consensus yet on the topic.
>
> Thank you for the information that you can provide and hope to see you in
> WL6.
>
> Best regards,
> Hugo O. Valdivia,  BSc, PhD
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