[Leish-l] Confirm hits to Leishmania spp. in whole blood of mammals in the US?

[Lucio Bouty]

Good evening gentlemen,
I wonder if someone has in its files a good kennel model or dogs facilities
designed to animal testing in treatment for visceral leishmaniasis ??? My
advisor is preparing a research project that aims to treat dogs groups with
molecules extracted from plants in our laboratory.
If anyone can help us we would be delighted

2015-03-05 20:30 GMT-03:00 Sayler,Katherine A <saylerk at ufl.edu>:

>  Dear readers,
>
>
>  Thank you sincerely for your time. I am a tick-borne disease biologist
> by trade, so this is outside of my realm.
>
>
>  I am trying to confirm some preliminary data that suggests the
> *Leishmania* spp. are circulating in limited geographic locales in
> southern Florida. Is it at all reasonable to expect to detect the DNA of
> the trypanosomatids in the blood of infected wildlife? My suspicion is that
> this is completely improbable, as it appears that in order to detect DNA in
> enough quantity or quality for sequencing, you need cultured organisms.
> From sandflies it appears that it is possible to PCR amplify and sequence
> directly from the vector, but never from the reservoir (or potential
> reservoir) host. Is this true? Any advice or insight would be greatly
> appreciated and I am tremendously naïve and this is clearly not my area of
> expertise.
>
>
>  Thank you again for reading my message,
>
> Katherine
>
>  Katherine Sayler, M.Ed., Ph.D.
> Postdoctoral Associate | Department of Wildlife Ecology and Conservation
> Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611
> Phone: 352-871-3259
>
>
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-- 
*Lucio **Flavio Marinho Bouty*
*Medico Veterinário*
CRMV-CE nº 1672
*Área de atuação: Clínica médica (Nefrologia) e Cirurgia geral (Urologia)*
*NEFROVET* - Consultoria em nefrologia e urologia veterinária, acessos
vasculares, ostomias, nutrição enteral e parenteral
*Staff de Cirurgia Geral do Hospital Veterinário da Faculdade de
Veterinária - UECE*
Sócio-fundador *CDV - Suporte Veterinário Intensivo*
Membro do *GRUPO DE ESTUDO E PESQUISA EM NEFROLOGIA E HEMODIÁLISE
VETERINÁRIAS - UFMG*
Especialização em Saúde Pública (Gestão em Vigilância Sanitária) - ESP - CE
Mestrando em Ciências Veterinárias (Sanidade Animal) - PPGCV/FAVET/UECE
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