<div dir="ltr"><div class="gmail_default" style="font-family:'comic sans ms',sans-serif"><div class="gmail_default">Good evening gentlemen,</div><div class="gmail_default">I wonder if someone has in its files a good kennel model or dogs facilities designed to animal testing in treatment for visceral leishmaniasis ??? My advisor is preparing a research project that aims to treat dogs groups with molecules extracted from plants in our laboratory.</div><div class="gmail_default">If anyone can help us we would be delighted</div></div></div><div class="gmail_extra"><br><div class="gmail_quote">2015-03-05 20:30 GMT-03:00 Sayler,Katherine A <span dir="ltr"><<a href="mailto:saylerk@ufl.edu" target="_blank">saylerk@ufl.edu</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p>Dear readers, </p>
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<p>Thank you sincerely for your time. I am a tick-borne disease biologist by trade, so this is outside of my realm.
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<font size="3" face="Calibri"><span style="font-size:12pt"><span dir="ltr"><span style="background-color:white"></span></span></span></font></p>
<p><font size="3" face="Calibri"><span style="font-size:12pt"><span dir="ltr"><span style="background-color:white">I am trying to confirm some preliminary data that suggests the
<em>Leishmania</em> spp. are circulating in limited geographic locales in southern Florida. </span><font size="3"><span style="background-color:white">Is it at all reasonable to expect to detect the DNA of the trypanosomatids in the blood of infected wildlife?
My suspicion is that this is completely improbable, as it appears that in order to detect DNA in enough quantity or quality for sequencing, you need cultured organisms. From sandflies it appears that it is possible to PCR amplify and sequence directly from
the vector, but never from the reservoir (or potential reservoir) host. Is this true? Any advice or insight would be greatly appreciated and I am tremendously naïve and this is clearly not my area of expertise</span></font></span></span></font>.<br>
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<p>Thank you again for reading my message,</p>
<p>Katherine<br>
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<div><font face="Arial">Katherine Sayler, M.Ed., Ph.D.</font></div>
<div><font face="Arial">Postdoctoral Associate | Department of Wildlife Ecology and Conservation</font></div>
<div><font face="Arial">Molecular Ecology Laboratory | 2322 Mowry Rd. Gainesville, FL 32611</font></div>
<div><font face="Arial">Phone: <a href="tel:352-871-3259" value="+13528713259" target="_blank">352-871-3259</a> </font></div>
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<br></blockquote></div><br><br clear="all"><div><br></div>-- <br><div class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div style="text-align:center"><font size="4"><b>Lucio </b><strong>Flavio Marinho Bouty</strong></font></div><div style="text-align:center"><font size="4"><strong>Medico Veterinário</strong></font></div><div style="text-align:center">CRMV-CE nº 1672</div><div style="text-align:center"><span style="background-color:rgb(255,255,255)"><b>Área de atuação: Clínica médica (Nefrologia) e Cirurgia geral (Urologia)</b></span></div><div style="text-align:center"><font style="background-color:rgb(255,255,255)" color="#000000"><b>NEFROVET</b> - Consultoria em nefrologia e urologia veterinária, acessos vasculares, ostomias, nutrição enteral e parenteral</font></div><div style="text-align:center"><font style="background-color:rgb(255,255,255)" color="#000000"><b>Staff de Cirurgia Geral do Hospital Veterinário da Faculdade de Veterinária - UECE</b></font></div><div style="text-align:center"><font style="background-color:rgb(255,255,255)" color="#000000">Sócio-fundador <b>CDV - Suporte Veterinário Intensivo</b></font></div><div style="text-align:center">Membro do <b>GRUPO DE ESTUDO E PESQUISA EM NEFROLOGIA E HEMODIÁLISE VETERINÁRIAS - UFMG</b></div><div style="text-align:center">Especialização em Saúde Pública (Gestão em Vigilância Sanitária) - ESP - CE</div><div style="text-align:center">Mestrando em Ciências Veterinárias (Sanidade Animal) - PPGCV/FAVET/UECE</div><div> </div></div></div></div></div></div></div>
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