[Leish-l] S.O.S.

[Joachim Clos]

Dear Rita,
we have some limited experience using L. braziliensis strains MHOM/PE/2002/PER104 (PER104) and MHOM/PE/2001/PER002 (PER002). See the attached paper by Andrea Nuehs and others:
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Nuehs et al, 2014.pdf
Type: application/pdf
Size: 1037455 bytes
Desc: not available
URL: <http://lineu.icb.usp.br/pipermail/leish-l/attachments/20140305/0535fa45/attachment-0001.pdf>
-------------- next part --------------

I hope this helps. Please keep in mind that we did not have the best experiences using episomal plasmid-based transgenes. Whilst the cosmids performed mostly to expectation, smaller episomes may have caused RNAi effects (see the attached paper). G418 sensitivity also varies from isolate to isolate and must be determined first.

On 18 Feb 2014, at 04:40, Rita Mukhopadhyay <rmukhop at fiu.edu> wrote:

> Dear Colleagues,
> 
> Can anyone please send me transfection protocols for braziliensis and panamensis?  We are trying with G418 selection.  Looks like they are too sensitive to the regular 1500V electroporation protocol.
> 
> Thanks a lot for your help.
> 
> Rita Mukhopadhyay
> _______________________________________________
> Leish-l mailing list
> Leish-l at lineu.icb.usp.br
> http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l
> 
> --
> This email was sent by icb.usp.br