[Leish-l] S.O.S.

Wed Mar 5 19:28:08 BRT 2014

Dear Rita, 

We had no problem using high voltage to transfect L. braziliensis and L. panamensis. You can find this information here: 

Mol Microbiol. 2013 Feb;87(3):580-93. doi: 10.1111/mmi.12117. Epub 2012 Dec 26.
The structure and repertoire of small interfering RNAs in Leishmania (Viannia) braziliensis reveal diversification in the trypanosomatid RNAi pathway.
Atayde VD1, Shi H, Franklin JB, Carriero N, Notton T, Lye LF, Owens K, Beverley SM, Tschudi C, Ullu E.

Yours,

Vanessa Atayde

________________________________________
From: leish-l-bounces at lineu.icb.usp.br [leish-l-bounces at lineu.icb.usp.br] on behalf of Rita Mukhopadhyay [rmukhop at fiu.edu]
Sent: Monday, February 17, 2014 10:40 PM
To: Patrick Bastien; leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] S.O.S.

Dear Colleagues,

Can anyone please send me transfection protocols for braziliensis and panamensis?  We are trying with G418 selection.  Looks like they are too sensitive to the regular 1500V electroporation protocol.

Thanks a lot for your help.

Rita Mukhopadhyay
_______________________________________________
Leish-l mailing list
Leish-l at lineu.icb.usp.br
http://lineu.icb.usp.br/cgi-bin/mailman/listinfo/leish-l

--
This email was sent by icb.usp.br