[Leish-l] Induction of LST positivity by BCG vaccination

Kwang-Poo Chang kwangpoo.chang at rosalindfranklin.edu
Wed Feb 5 10:57:04 BRST 2014


Dear Farrokh,

Thanks a lot for the information. I'll come back to you later .

KP


On Wed, Feb 5, 2014 at 1:33 AM, Farrokh Modabber <fmodabber at dndi.org> wrote:

>  Dear KP,
>
> Both leishmanin and BCG we used were produced at Pasteur Inst, Iran.
> Leishmanin is from a well characterized *L. major* promastigotes.  Dr
> Alimohammadian was in charge of leishmanin and he is cc'ed if you like to
> contact him.  I am sure Ali knows about it too but I thought dr
> Alimohammadian might tell you the specifics or send you his SOP.  There are
> several BCG's (Pasteur Institute Paris, Staten Institute Denmark, one from
> Japan, and others).  We tested several and decided on the Pasteur Institute
> of Iran because of its moderate reactogenicity, cost and availability).
>  The injection is 0.1 ml (if I remember correctly containing about 105/ml) i.d. usually in the forearm and read between 48-72 hrs later for
> induration using the ball-point method.  We have also used autoclaved
> promastigotes(the first generation vaccine) as leishmanin and it also
> worked!
>
> Hope you will get more specific info from other colleagues.
>
> Best regards,
>
> Farrokh
>
>
>
> *From:* Kwang-Poo Chang [mailto:kwangpoo.chang at rosalindfranklin.edu]
> *Sent:* Tuesday, February 04, 2014 10:57 AM
> *To:* Ali Khamesipour
> *Cc:* Farrokh Modabber; Leish-l at lineu.icb.usp.br
> *Subject:* Re: [Leish-l] Induction of LST positivity by BCG vaccination
>
>
>
> Dear Ali,
>
>
>
> Could you give me the information for the source of BCG used and also the
> protocol for preparing materials for the leishmanin test ? I recall the
> materials are either Leishmania promastigotes or Crithidia preserved in 1%
> phenol ? I could be totally wrong. I don't remember the species and cell
> density or number, etc. Also, the volume and site of injection...........
>
>
>
> Thank you in advance for your consideration of these requests.
>
>
>
> KP
>
>
>
> On Fri, Jan 10, 2014 at 8:47 AM, Ali Khamesipour <
> khamesipour_ali at yahoo.com> wrote:
>
> Dear All,
> Dr Modabber you should well remember that in the trials in Iran we had a
> degree of LST conversion in individuals who were originally LST negative
> (no response to leishmanin; no induration and no erythema), the control
> group received BCG, and a percent of them converted to LST positive.
> All the best Ali
>
>
>
>
>
> Dr Ali Khamesipour
>
>
>
> On Friday, January 10, 2014 11:22 AM, Farrokh Modabber <fmodabber at dndi.org>
> wrote:
>
> Dear Colleagues,
>
> I recall from Prof. Mayrinks studies, that BCG vaccination may convert
> leishmanin skin test in some volunteers who are LST-negative
> (MST-negative). The reactivity would gradually diminish after a few
> months.  Would appreciate any information on that and on Prof. Mayrink's
> current email address.
>
> Many  thanks,
>
> Farrokh Modabber
>
>
>
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