[Leish-l] why is Leishmania donovani restricted to humans in Indian subcontinent?

[Stefano Reale]

Hallo Im one of the author of this PCR protocol. I optimised it by specific
primers and probe for infantum. It work very well and I showed the Lod and
Loq in variuos biological material taken from leishmaniotic dogs. In my lab
in Palermo I have a number if collected strains stored in liquid N2 ready
to create the DNA standard referement serial dilutions fir Real time test.
I could help who should have these DNA.
Best regards
Il giorno 31/ott/2013 00:07, "Kwang-Poo Chang" <
kwangpoo.chang at rosalindfranklin.edu> ha scritto:

> Dear Carlos,
>
> One of my colleagues in Naples, Italy has used primer set for kinetoplast
> minicircle conserved region, Considering ~10,000 copies of minicircvles per
> Leishmania, this is probably the most sensitive PCR, although the primer
> set can also amply all trypanosomes or trypanosomatid protozoa (including
> Leptomonas reported to exist in the Indian kala-azar splenic aspirates).
> Specificity could be an issue in that sense.
>
> KP
>
>
> On Thu, Oct 10, 2013 at 7:31 PM, Carlos Lobo <carloshlobo at gmail.com>wrote:
>
>> Hello guys, good night.
>> I'm taking advantage of this opportunity to exchange scientific
>> information, much of which I have learned to ask for help.
>> I'll start a project on canine leishmaniasis in northeastern Brazil and I need
>> to diagnose, by PCR, dogs infected and not infected with Leish.
>> I've been doing some analysis of PCR for other experiments, but never did
>> for Leish, could someone give me some tips? Like which primer to use?
>> Should I collect blood or tissue samples to have more reliability?
>> Thank you.
>> Carlos Henrique
>>
>>
>> 2013/9/23 Tamrat Abebe Zeleke <tabebezeleke at gmail.com>
>>
>>> Dear Carlos,
>>>
>>> I agree with the comment given by Phillip Lawyer. The molecular analysis
>>> of strains or isolates from India, Kenya, and South Western Ethiopia also
>>> supports this notion. However, the issue of distinct strains in Sudan and
>>> North Ethiopia opts for the fact that East Africa may be the origin of at
>>> least the naughty L. donovani strains circulating in the region.
>>>
>>> Tamrat Abebe
>>> Addis Ababa University College of Health Sciences, School of Medicine ,
>>> Department of Microbiology, Immunology & Parasitology
>>> Tikur Anbessa Hospital
>>> Second floor room number 76
>>> Tel: +251 911 447227(mobile)
>>> Email: tamrat.abebe at aau.edu.et
>>> *
>>> *
>>> *
>>> *
>>>
>>>
>>>
>>>
>>> On Mon, Sep 16, 2013 at 4:37 AM, Lawyer, Phillip (NIH/NIAID) [E] <
>>> PhillipL at niaid.nih.gov> wrote:
>>>
>>>> Dear Carlos,
>>>>
>>>>
>>>>
>>>> For what it's worth, I believe it most likely happened the other way
>>>> around:  Leishmania donovani was probably introduced to East Africa from
>>>> India during the late 1800s when laborers were brought from India to work
>>>> in the Kenya building the railroad from Mombasa to Uganda and on other
>>>> infrastructure projects.  Leishmania donovani in East Africa is manifest as
>>>> kala azar and is anthroponotic, the same as in India.  The main vector in
>>>> Kenya is Phlebotmus martini, which tends to breed in termite mounds, often
>>>> associated with human dwellings.  Other Symphlebotomus species, Ph.
>>>> vansomerenae and Ph. celiae have also been implicated in L. donovani
>>>> transmission.
>>>>
>>>>
>>>>
>>>> Regards,
>>>>
>>>>
>>>>
>>>> Phil Lawyer
>>>>
>>>> ________________________________
>>>> From: Carlos Brisola Marcondes [cbrisolamarcondes at gmail.com]
>>>> Sent: Friday, August 30, 2013 8:41 AM
>>>> To: Leish-L; leish-l-bounces at lineu.icb.usp.br
>>>> Subject: [Leish-l] why is Leishmania donovani restricted to humans in
>>>> Indian subcontinent?
>>>>
>>>> Dear all,
>>>> Leishmania donovani seems to have been introduced from East Africa to
>>>> Indian subcontinent, where it has infected mostly humans, differently from
>>>> East African foci.
>>>>    Why does this occur? Is this caused by feeding preferences of
>>>> Phlebotomus argentipes, which bites mostly ruminants and humans and is
>>>> associated to houses? Or are dogs rarer in that region than in Brazil,
>>>> where these animals are important reservoirs of Leishmania infantum and
>>>> frequently bitten by Lutzomyia longipalpis?
>>>>
>>>> Sincerely yours
>>>> prof. dr. Carlos Brisola Marcondes
>>>> Dept. Microbiol. Imunol. Parasitol./CCB
>>>> Federal University of Santa Catarina
>>>> Florianópolis (SC)
>>>> CV:
>>>> http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783901J2
>>>> blog: http://entomomedica.blogspot.com.br/
>>>>
>>>>
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>>>
>>>
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