[Leish-l] Fwd: hot spots

danz at bi.technion.ac.il danz at bi.technion.ac.il
Sat Mar 30 12:30:51 BRT 2013


Hi Sunil,
May I add to Peter's remarks. We and others showed that axenic differentiation initiates only when promastigotes expose to both high temperature and acidic pH. If stress was the signal, temperature or pH alone could induce differentiation. One important feature of the host-free or axenic system is that we can perform time course analysis of the process of differentiation, thereby evaluate/reveal pathways responsible for Leishmania development. This is something you cannot do neither in macrophages nor in animal models. In vivo validation can follow experiment in axenic system. Finally, in papers that we have published to date, some of which were done in collaboration with Peter, we demonstrated that signal-induced differentiation in axenic conditions is highly regulated process and reasonable representation of intracellular amastigotes. We will soon publish a paper that for the first time reveals the signaling pathway that initiates promastigote to amastigote differentiation.  My graduate student will reveal this pathway in her talk at WL5.

The axenic system is the best in vitro system that simulate differentiation. Finally, do not forget that the culture promastigotes all of us use are axenic promastigotes...

See you all in May

Dan

Professor Dan Zilberstein, Executive director
Center for Pre Academic Education
And The Faculty of Biology
Technion-Israel Institute of Technology
Haifa 32000, Israel

ב-29 במרץ 2013, בשעה 16:09, "Sunil Arora" <skarora_in at yahoo.com<mailto:skarora_in at yahoo.com>> כתב/ה:

I agree...axenic amastigotes are more like stressed out promastigotes... I always prefer working with something inside the macrophages as a model for testing drugs or immunomodulators which I believe are closer to actual intracellular amastigotes in the infected host

best
sunil

---------------------------------------------------------------
Dr Sunil K.Arora
Professor
In Charge- HIV Diagnostic and Disease Monitoring Laboratory &
NACO State Reference Laboratory,

Councillor- Federation of Immunological Societies of Asia-oceania (FIMSA)
Vice President- Indian Immunology Society (IIS)
Secretary (Research)- The Cytometry Society of India (TCS)

Department of Immunopathology
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________________________________
From: Clos Joachim <clos at bni-hamburg.de<mailto:clos at bni-hamburg.de>>
To: Leish-L email post <Leish-l at lineu.icb.usp.br<mailto:Leish-l at lineu.icb.usp.br>>
Sent: Monday, 11 March 2013 8:16 PM
Subject: [Leish-l] Fwd: hot spots

Are axenic amastigotes a valid model (and for what?), the first developmental step towards real amastigotes, or just stressed-out promastigotes? Many of us use axenics and swear by them, others are skeptical, others will accept what's inside a macrophage, still others only want amastigotes isolated from something with a fur. I believe we should come to some sort of common language and interpretation. I am somewhere in the middle myself, preferring to do things in macrophages whenever possible.
Cheers!



Am 07.02.2013 um 20:38 schrieb Carlos Costa:

What are the most polemic issues in leishmaniasis?

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