[Leish-l] luciferase-transfected Leishmania

[Grégory Michel]

Dear Goto,
I have stably transfected Leishmania infantum with both luciferase or GFP gene with the support of T. Lang of Pasteur Institut of Paris. The insertion of the luciferase or GFP gene are into the DNA coding for the 18S sub-unit.  I'have already used both of them in vivo and in vitro. 
It's possible to send you the strain if you agree to sign a MTA vith Pasteur Institut of Paris.
Let me know
Best regards

Grégory


Grégory Michel, PhD
Bâtiment Universitaire Archimed 
INSERM U1065, C3M 
151 Route de Ginestière 
BP 2 3194 
F-06204 NICE Cedex 3
France


Tel.: 04 89 06 42 45
Fax : 04 89 06 42 21
e-mail : 
gmichel at unice.fr

Le 15 mai 2012 à 18:23, Hiro Goto a écrit :

> Dear Shaw,
> We would like to work with in vivo immaging in leishmaniasis in mouse model, and instead of GFP expressing parasites, luciferase-transfected Leishmania would be desirable. We are interested for the moment in L. major and L. chagasi/infantum. Is there any group that could provide us with these transfected Leishmania, preferentially from Brazil, because to receive from abroad the burocracy is very very complex.
> Best regards,
> Hiro
> 
> Profa. Dra. Hiro Goto
> Laboratório de Soroepidemiologia e Imunobiologia
> Instituto de Medicina Tropical de São Paulo, USP
> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
> 05403-000 - São Paulo, SP
> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
> Fax. +55-11-3061-8270
> 
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