[Leish-l] L. infantum in the hindgut?

[Wilson, Mary E]

See Jason Weirather's publication below.  We have some primers in there that distinguish between L. Leishmania spp. and L. Viannia spp.
Mary Wilson

Weirather, J. L., S. M. B. Jeronimo, S. Gautam, S. Sundar, M. Kang, M. A. Kurtz, R. Haque, A. Schriefer, S. Talhari, E. M. Carvalho, J. E. Donelson and M. E. Wilson.  Serial quantitative PCR assay for detection, species-discrimination and quantification of Leishmania spp. in human samples.  J. Clin. Micro. 49(11):382-3904, 2011
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From: leish-l-bounces at lineu.icb.usp.br [leish-l-bounces at lineu.icb.usp.br] on behalf of Chang, Kwang-Poo [KwangPoo.Chang at rosalindfranklin.edu]
Sent: Saturday, June 09, 2012 1:55 PM
To: Carlos Costa; Leish-L; Regiane Araujo; IVETE LOPES DE MENDONCA
Subject: Re: [Leish-l] L. infantum in the hindgut?

Nagt sequence analysis can easily differentiate Viannia from Leishmania.  You will find this in our publication in 2007 that has been verified by additional data generated by Carlos Muskus, Medellin.

Genes encoding protein elements for the RNAi machinery are expected to exit only in the Viannia group, but I have not looked into this by designing primers for the ease of their PCR amplification versus nagt. These genes are expected to be absent in the Leishmania subgenus, producing no PCR products.  Such negative findings however may not be as good as something positive in both groups, but different between them.

KP

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From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Carlos Costa
Sent: Saturday, June 02, 2012 5:28 PM
To: Leish-L; Regiane Araujo; IVETE LOPES DE MENDONCA
Subject: [Leish-l] L. infantum in the hindgut?

Dear all,

Dr. Ivete Mendonça, in Teresina, Brazil, has consistently and systematically been observing promastigotes xenodiagnosis performed in dogs in the hindgut and rectal ampulla of Lutzomyia longipalpis. We are not sure if they are really L. infantum or Viannia. Does anybody has observed the same thing?

By the way, any suggestions of primers to discriminate Leishmania from Viannia?

Many thanks,

Carlos.




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