[Leish-l] inquiry

Sat May 21 20:39:44 BRT 2011

Dr. NL Sharma has been working on an important CL endemic area along the Satluj River valley to the south of Himalaya in India (Please correct me should I be wrong for anything I said here and below). I had the good fortune of visiting the site several years back courtesy of Dr. Sharma. 

I believe Dr. Sharma's finding is important, since the parasites there are different from the familiar Indian L donovani in Bihar. The parasites are refratory to in vitro cultivation. They do differentiate into promastigotes and may grow a little, but  can't really be subcultured to establish passageable lines. This is very much reminescent of L infantum in the Mediterranean area. I recall Dr. Sharma has also found rK39+ dogs, if I remember correctly. If so,  Satluj river valley is endemic to the infantile CL.

Analyses of several batches of basically clinical CL samples from Dr. Sharma showed evidence of L infantum, but also L tropica as well as a mixture of the two in one sample. This is the same picture we have noted for samples from Hatay, Turkey.

These observations make me wonder a lot about our current knowledge on the clinico-epidemiology based on data collected previously by analyses of cultured promastigotes from one or few 'representative samples'. Nowaday, technology makes it very doable to work with biological samples for Leish DNAs directly from sand flies, patients and reservoir animals.

KP

________________________________

From: leish-l-bounces at lineu.icb.usp.br on behalf of Sharman
Sent: Fri 5/20/2011 11:31 PM
To: Hiro Goto; elfadil abass
Cc: leish-l at lineu.icb.usp.br
Subject: Re: [Leish-l] inquiry

Dear all
I agree with Hiro gito, We are working on a focus where the CL is
predominantly caused by L. donovani, and the rK 39 STRIPS GIVE POSITIVE
RESULTS WITH SERA as well as serous exudate from the lesion. The results are
dependent upon species.
NL Sharma

--------------------------------------------------
From: "Hiro Goto" <hgoto at usp.br>
Sent: Wednesday, May 18, 2011 12:09 AM
To: "elfadil abass" <elfadil_abass at yahoo.com>
Cc: <leish-l at lineu.icb.usp.br>
Subject: Re: [Leish-l] inquiry

> Dear all,
> In our oppinion, DAT and rK39 for those samples are not indicated  since
> these tests are produced for the diagnosis of visceral  leishmaniasis.
> In case of tegumentary leishmaniasis, it is very appropriate the
> observation of J.J. Shaw appointing species specificity of antibody
> response in these cases. Titers of antibodies are in general low in  these
> cases therefore depending on the species, it may result  negative. We have
> published a review recently in Expert Rev. Anti  Infect. Ther. 8(4),
> 419?433 (2010), Current diagnosis and treatment of  cutaneous and
> mucocutaneous leishmaniasis, where we raise this point.
> Hiro Goto
>
> Citando elfadil abass <elfadil_abass at yahoo.com>:
>
>> Dear all I would recommend using DAT and rK39 strip test to measure
>> antibody
>> responses and to evaluate the diagnostic efficiency for both tests  in
>> such group
>> of patients.
>>
>>  Elfadil Abass
>> ________________________________________________________________________________
>> Institute of Medical Microbiology and Hospital Hygiene
>> Philipps University Marburg
>> BMFZ / Hans-Meerwein Straße 2
>> D-35033 Marburg, Germany
>> ________________________________________________________________________________
>>
>>
>>
>>
>> ________________________________
>> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
>> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
>> <leish-l at lineu.icb.usp.br>
>> Sent: Fri, May 13, 2011 9:39:33 AM
>> Subject: Re: [Leish-l] inquiry
>>
>> Dear Saad first precaution to take is to separate your samples into
>> several
>> portions each (at least 5 each being no more than a few hundred(200-400)
>> microliters). This is a necessary precaution to avoid freezing and
>> thawing
>> several times the same sample which will be the case should you do
>> different
>> studies at different times which is unavoidable.
>>
>> What you can do is correlate the type(ulcerated, abscess, furuncle
>>  etc.. ) the
>> locale, the number and age of lesions with the levels of antibody in  the
>> sera of
>> these patgients and also monitor cell mediated immunity with leishmanin
>> skin
>> test!
>> I have just published (in print) an article about having circulating
>> parasites
>> in some of these patients with apparently pure cutaneous disease. It is
>> the
>> first paper that revealed this fact. It would be interesting to confirm
>> or
>> de-confirm this fact repeating what we did.
>> Best wishes
>>
>> Nuha Nuwayri-Salti MD
>>
>>
>>
>> AOA
>> Medical Honor Society
>>
>>
>>
>>
>> -----Original Message-----
>> From: leish-l-bounces at lineu.icb.usp.br
>> [mailto:leish-l-bounces at lineu.icb.usp.br]
>> On Behalf Of saad saad
>> Sent: Friday, April 29, 2011 11:23 PM
>> To: leish-l at lineu.icb.usp.br
>> Subject: [Leish-l] inquiry
>>
>> Dear all
>> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
>> CL from south west of the country. I would like to have your
>> recommendations to start a good research line in CL using these sera.
>> Thanks in advance for your help
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>
>
>
> Profa. Dra. Hiro Goto
> Laboratório de Soroepidemiologia e Imunobiologia
> Instituto de Medicina Tropical de São Paulo, USP
> Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
> 05403-000 - São Paulo, SP
> Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
> Fax. +55-11-3061-8270
>
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