[Leish-l] inquiry

[Hiro Goto]

Dear all,
In our oppinion, DAT and rK39 for those samples are not indicated  
since these tests are produced for the diagnosis of visceral  
leishmaniasis.
In case of tegumentary leishmaniasis, it is very appropriate the  
observation of J.J. Shaw appointing species specificity of antibody  
response in these cases. Titers of antibodies are in general low in  
these cases therefore depending on the species, it may result  
negative. We have published a review recently in Expert Rev. Anti  
Infect. Ther. 8(4), 419?433 (2010), Current diagnosis and treatment of  
cutaneous and mucocutaneous leishmaniasis, where we raise this point.
Hiro Goto

Citando elfadil abass <elfadil_abass at yahoo.com>:

> Dear all I would recommend using DAT and rK39 strip test to measure antibody
> responses and to evaluate the diagnostic efficiency for both tests  
> in such group
> of patients.
>
>  Elfadil Abass
> ________________________________________________________________________________
> Institute of Medical Microbiology and Hospital Hygiene
> Philipps University Marburg
> BMFZ / Hans-Meerwein Straße 2
> D-35033 Marburg, Germany
> ________________________________________________________________________________
>
>
>
>
> ________________________________
> From: Nuha Nuwayri-Salti <racha at aub.edu.lb>
> To: saad saad <saad1426 at gmail.com>; "leish-l at lineu.icb.usp.br"
> <leish-l at lineu.icb.usp.br>
> Sent: Fri, May 13, 2011 9:39:33 AM
> Subject: Re: [Leish-l] inquiry
>
> Dear Saad first precaution to take is to separate your samples into several
> portions each (at least 5 each being no more than a few hundred(200-400)
> microliters). This is a necessary precaution to avoid freezing and thawing
> several times the same sample which will be the case should you do different
> studies at different times which is unavoidable.
>
> What you can do is correlate the type(ulcerated, abscess, furuncle  
> etc.. ) the
> locale, the number and age of lesions with the levels of antibody in  
> the sera of
> these patgients and also monitor cell mediated immunity with leishmanin skin
> test!
> I have just published (in print) an article about having circulating  
> parasites
> in some of these patients with apparently pure cutaneous disease. It is the
> first paper that revealed this fact. It would be interesting to confirm or
> de-confirm this fact repeating what we did.
> Best wishes
>
> Nuha Nuwayri-Salti MD
>
>
>                                                                        AOA
> Medical Honor Society
>
>
>
>
> -----Original Message-----
> From: leish-l-bounces at lineu.icb.usp.br  
> [mailto:leish-l-bounces at lineu.icb.usp.br]
> On Behalf Of saad saad
> Sent: Friday, April 29, 2011 11:23 PM
> To: leish-l at lineu.icb.usp.br
> Subject: [Leish-l] inquiry
>
> Dear all
> Hi. i am Saad from Saudi Arabia. i have 60 sera from positive case of
> CL from south west of the country. I would like to have your
> recommendations to start a good research line in CL using these sera.
> Thanks in advance for your help
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>



Profa. Dra. Hiro Goto
Laboratório de Soroepidemiologia e Imunobiologia
Instituto de Medicina Tropical de São Paulo, USP
Av. Dr. Enéas de Carvalho Aguiar, 470, prédio II, quarto andar
05403-000 - São Paulo, SP
Tel. +55-11-3061 7023, 3061 7056 ou 3061 7027
Fax. +55-11-3061-8270