[Leish-l] miltefosine for diffuse leishmaniasis

[Carlos Costa]

Hi KP,

Easily. A skin biopsy is rich in amastigotes. How do you suggest to
conserve? Frozen? Formalin?

Cheers,

Carlos.

2011/7/13 Chang, Kwang-Poo <KwangPoo.Chang at rosalindfranklin.edu>

> ** **
>
> Dear Carlos,****
>
> ** **
>
> Is there anyway to collect some Leishmania in the clinical sample for
> genetic analysis/species identification without cultivation ? I still think
> it is important to do this just to make sure that cultivation does not
> select something clinically irrelevant.  If so, testing their
> drug-sensitivity may or may not be informative.  I don’t know if the lesions
> contain abundant amastigotes or infected macrophages for ex vivo evaluation
> of their drug-sensitivity. If so, one could think of several different ways
> to do it in micro-scale.****
>
> ** **
>
> KP****
>
> ** **
>  ------------------------------
>
> *From:* leish-l-bounces at lineu.icb.usp.br [mailto:
> leish-l-bounces at lineu.icb.usp.br] *On Behalf Of *Silvia Uliana
> *Sent:* Sunday, July 10, 2011 7:15 PM
> *To:* Carlos Costa
> *Cc:* Leish-L
>
> *Subject:* Re: [Leish-l] miltefosine for diffuse leishmaniasis
> ****
>
>  ** **
>
> Dear Carlos
>
> One of Dr. Bryceson's suggestions was to determine the sensitivity in
> vitro of isolate(s) obtained from this patient. We have been doing this in
> the lab (here in ****Sao Paulo****) and could easily determine the
> sensitivity of cultures taken from this patient.
> We would be ready to determine the sensitivity to antimonials, amphotericin
> B and miltefosine. We do not have paromomycin or pentamidin at present, but
> I'm sure we can find them somewhere. Just let me know...
>
> Abraço
>
> Silvia
>
>
> *****************************************************************************************
> Dra. Silvia Reni Bortolin Uliana
> Departamento de Parasitologia
> Instituto de Ciências Biomédicas
> Universidade de São Paulo
> Av. Prof. Lineu Prestes, 1374
> Tel: 11-30917334****
>



-- 
*Carlos H. N. Costa, MD, DSc.*
*President*
*Sociedade Brasileira de Medicina Tropical *
***(Brazilian Society of Tropical Medicine)*
*Instituto de Doenças Tropicais Natan Portella
Universidade Federal do Piauí*
*Rua Artur de Vasconcelos 151-Sul*
*64049-750 Teresina-PI
Brazil
Telephones: +55 86 3221-3413 (work); +55 86 8838-3303 (mobile).
*
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