[Leish-l] miltefosine for diffuse leishmaniasis
Chang, Kwang-Poo
KwangPoo.Chang at rosalindfranklin.edu
Wed Jul 13 12:46:11 BRT 2011
Dear Carlos,
Is there anyway to collect some Leishmania in the clinical sample for genetic analysis/species identification without cultivation ? I still think it is important to do this just to make sure that cultivation does not select something clinically irrelevant. If so, testing their drug-sensitivity may or may not be informative. I don't know if the lesions contain abundant amastigotes or infected macrophages for ex vivo evaluation of their drug-sensitivity. If so, one could think of several different ways to do it in micro-scale.
KP
________________________________
From: leish-l-bounces at lineu.icb.usp.br [mailto:leish-l-bounces at lineu.icb.usp.br] On Behalf Of Silvia Uliana
Sent: Sunday, July 10, 2011 7:15 PM
To: Carlos Costa
Cc: Leish-L
Subject: Re: [Leish-l] miltefosine for diffuse leishmaniasis
Dear Carlos
One of Dr. Bryceson's suggestions was to determine the sensitivity in vitro of isolate(s) obtained from this patient. We have been doing this in the lab (here in Sao Paulo) and could easily determine the sensitivity of cultures taken from this patient.
We would be ready to determine the sensitivity to antimonials, amphotericin B and miltefosine. We do not have paromomycin or pentamidin at present, but I'm sure we can find them somewhere. Just let me know...
Abraço
Silvia
*****************************************************************************************
Dra. Silvia Reni Bortolin Uliana
Departamento de Parasitologia
Instituto de Ciências Biomédicas
Universidade de São Paulo
Av. Prof. Lineu Prestes, 1374
Tel: 11-30917334
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