[leish-l] Iran DNA progress 10-6-03

[K.P. Chang]

Dear all,

Sure thing !

My student, Kayoko Waki, randomly picked three samples and used 1 & 5 ul as
templates for PCR amplificaiton of nagt. All produced single bands of ~1.4 kb,
indicating that the DNAs are of good quality, although there are variable band
intensity, suggestive of different centrations. We plan to complete the PCR by
adjusting the DNA amounts for RFLP analysis.

KP

modabberf at who.int wrote:

> Glad all worked out.  I will look forward to seeing your results.
> Regards,
> Farrokh
>
> -----Original Message-----
> From: K.P. Chang [mailto:changk at mail.finchcms.edu]
> Sent: Saturday, 4 October 2003 01:51
> To: K.Chang at finchcms.edu
> Cc: Mehdi Mohebali; jeffreyj at usp.br; leish-l at fat.org.br;
> sima-rafatisy at institute.pasteur.ac.ir; Kayoko Waki; Farrokh Modabber
> Subject: [leish-l] DNAs Iran received 10-3-03
>
> Dear Dr. Mohebali and Dr. Modabber,
>
> Thank you very much for sending the Leish DNAs of most interesing isolates
> from
> various clinical forms, different sources and different places. They would
> seem to
> be representative of the current leishmanasis in iran.
>
> I have asked Kayoko, my student, to select several samples for PCR
> amplification of
> nagt. Should this work out well, she would then extend it to all ~20 samples
> for
> RFLP analysis. This analysis is expected to differentiate L infantum, L
> major, L
> tropica, L donovani, L turanica nagt based on sequences already available.
> The
> outcome can be firmed up by additional RFLP analysis. Should there be
> emergence of
> unexpected RFLP patterns. We would sequence the PCR products to determine if
> they
> might be new nagt genotypes.
>
> We will let you know the outcome in due course.
>
> Thank you again for taking the trouble of sending these most interesting
> samples.
>
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