[leish-l] DNAs Iran received 10-3-03

[modabberf at who.int]

Glad all worked out.  I will look forward to seeing your results.
Regards,
Farrokh

-----Original Message-----
From: K.P. Chang [mailto:changk at mail.finchcms.edu]
Sent: Saturday, 4 October 2003 01:51
To: K.Chang at finchcms.edu
Cc: Mehdi Mohebali; jeffreyj at usp.br; leish-l at fat.org.br;
sima-rafatisy at institute.pasteur.ac.ir; Kayoko Waki; Farrokh Modabber
Subject: [leish-l] DNAs Iran received 10-3-03


Dear Dr. Mohebali and Dr. Modabber,

Thank you very much for sending the Leish DNAs of most interesing isolates
from
various clinical forms, different sources and different places. They would
seem to
be representative of the current leishmanasis in iran.

I have asked Kayoko, my student, to select several samples for PCR
amplification of
nagt. Should this work out well, she would then extend it to all ~20 samples
for
RFLP analysis. This analysis is expected to differentiate L infantum, L
major, L
tropica, L donovani, L turanica nagt based on sequences already available.
The
outcome can be firmed up by additional RFLP analysis. Should there be
emergence of
unexpected RFLP patterns. We would sequence the PCR products to determine if
they
might be new nagt genotypes.

We will let you know the outcome in due course.

Thank you again for taking the trouble of sending these most interesting
samples.



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