[leish-l] What nagt seq supposed to show 10-10-03

[K.P. Chang]

Dear all,

Kayoko has been able to PCR-amplify nagt from all, except one of the samples
sent. She plans to carry out RFLP analysis of the PCR products based on the
restriction maps generated from the available sequences. Any apparently new nagt
demonstrated by this apporach will be sequenced to completion for comparison
against those already available, i.e. ~20 nagt genotypes. Hopefully, these data
will corroborate with the ongoing RAPD analysis data. We expect to discriminate
the Old World isolates, i. e. L tropica, L major,  L infantum/donovani, L
mexicana/amazonensis and L turanica (if present). The nagt sequence data are
100% consistent with previous species typing information, regardless of the
menthodology used. Nagt sequence heterogeneity within each of these five groups
in relation to clinico-epidemiology is our interest and remains to be sorted
out.

Regards.

KP

modabberf at who.int wrote:

> Glad all worked out.  I will look forward to seeing your results.
> Regards,
> Farrokh
>
> -----Original Message-----
> From: K.P. Chang [mailto:changk at mail.finchcms.edu]
> Sent: Saturday, 4 October 2003 01:51
> To: K.Chang at finchcms.edu
> Cc: Mehdi Mohebali; jeffreyj at usp.br; leish-l at fat.org.br;
> sima-rafatisy at institute.pasteur.ac.ir; Kayoko Waki; Farrokh Modabber
> Subject: [leish-l] DNAs Iran received 10-3-03
>
> Dear Dr. Mohebali and Dr. Modabber,
>
> Thank you very much for sending the Leish DNAs of most interesing isolates
> from
> various clinical forms, different sources and different places. They would
> seem to
> be representative of the current leishmanasis in iran.
>
> I have asked Kayoko, my student, to select several samples for PCR
> amplification of
> nagt. Should this work out well, she would then extend it to all ~20 samples
> for
> RFLP analysis. This analysis is expected to differentiate L infantum, L
> major, L
> tropica, L donovani, L turanica nagt based on sequences already available.
> The
> outcome can be firmed up by additional RFLP analysis. Should there be
> emergence of
> unexpected RFLP patterns. We would sequence the PCR products to determine if
> they
> might be new nagt genotypes.
>
> We will let you know the outcome in due course.
>
> Thank you again for taking the trouble of sending these most interesting
> samples.
>
> _______________________________________________
> leish-l mailing list
> leish-l at fat.org.br
> http://panda.fat.org.br/mailman/listinfo/leish-l
> _______________________________________________
> leish-l mailing list
> leish-l at fat.org.br
> http://panda.fat.org.br/mailman/listinfo/leish-l