[leish-l] DNAs Iran received 10-3-03

[K.P. Chang]

Dear Dr. Mohebali and Dr. Modabber,

Thank you very much for sending the Leish DNAs of most interesing isolates from
various clinical forms, different sources and different places. They would seem to
be representative of the current leishmanasis in iran.

I have asked Kayoko, my student, to select several samples for PCR amplification of
nagt. Should this work out well, she would then extend it to all ~20 samples for
RFLP analysis. This analysis is expected to differentiate L infantum, L major, L
tropica, L donovani, L turanica nagt based on sequences already available. The
outcome can be firmed up by additional RFLP analysis. Should there be emergence of
unexpected RFLP patterns. We would sequence the PCR products to determine if they
might be new nagt genotypes.

We will let you know the outcome in due course.

Thank you again for taking the trouble of sending these most interesting samples.